TY - JOUR

T1 - Fluorescence Imaging of Cell Membrane Potential: From Relative Changes to Absolute Values

AU - Николаев, Дмитрий Михайлович

AU - Миронов, Владимир Николаевич

AU - Штыров, Андрей Андреевич

AU - Квашнин, Ярослав Дмитриевич

AU - Мерещенко, Андрей Сергеевич

AU - Васин, Андрей Владимирович

AU - Панов, Максим Сергеевич

AU - Рязанцев, Михаил Николаевич

PY - 2023/1/26

Y1 - 2023/1/26

N2 - Membrane potential is a fundamental property of biological cells. Changes in membrane potential characterize a vast number of vital biological processes, such as the activity of neurons and cardiomyocytes, tumorogenesis, cell-cycle progression, etc. A common strategy to record membrane potential changes that occur in the process of interest is to utilize organic dyes or genetically-encoded voltage indicators with voltage-dependent fluorescence. Sensors are introduced into target cells, and alterations of fluorescence intensity are recorded with optical methods. Techniques that allow recording relative changes of membrane potential and do not take into account fluorescence alterations due to factors other than membrane voltage are already widely used in modern biological and biomedical studies. Such techniques have been reviewed previously in many works. However, in order to investigate a number of processes, especially long-term processes, the measured signal must be corrected to exclude the contribution from voltage-independent factors or even absolute values of cell membrane potential have to be evaluated. Techniques that enable such measurements are the subject of this review.

AB - Membrane potential is a fundamental property of biological cells. Changes in membrane potential characterize a vast number of vital biological processes, such as the activity of neurons and cardiomyocytes, tumorogenesis, cell-cycle progression, etc. A common strategy to record membrane potential changes that occur in the process of interest is to utilize organic dyes or genetically-encoded voltage indicators with voltage-dependent fluorescence. Sensors are introduced into target cells, and alterations of fluorescence intensity are recorded with optical methods. Techniques that allow recording relative changes of membrane potential and do not take into account fluorescence alterations due to factors other than membrane voltage are already widely used in modern biological and biomedical studies. Such techniques have been reviewed previously in many works. However, in order to investigate a number of processes, especially long-term processes, the measured signal must be corrected to exclude the contribution from voltage-independent factors or even absolute values of cell membrane potential have to be evaluated. Techniques that enable such measurements are the subject of this review.

KW - Cell Membrane/metabolism

KW - Fluorescent Dyes/metabolism

KW - Membrane Potentials/physiology

KW - Neurons/metabolism

KW - Optical Imaging

UR - https://www.mendeley.com/catalogue/1f0846db-1d86-3ab5-84bb-d5dac7b3175d/

U2 - 10.3390/ijms24032435

DO - 10.3390/ijms24032435

M3 - Review article

C2 - 36768759

VL - 24

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 3

M1 - 2435

ER -