We investigate laser treatment regimes suitable for the fabrication of the isolated cells in a porous silica glass and a solgel film. Three-dimensional structures are produced as a result of the porous glass densification induced by femtosecond laser pulses. We also demonstrate that CO₂ laser can be used to form such structures in the solgel film deposited on the glass. In the porous glass, barriers with various permeability were created, which were used as walls in fluid cells. Laser-induced microrelief formation on the surface of the solgel film provides isolation for the separated porous cells. The impermeability of porous cells is tested by impregnation them with different dyes (rhodamine 6G, fuchsine, etc.).