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Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin. / Gad, Helge; Ringstad, Niels; Löw, Peter; Kjaerulff, Ole; Gustafsson, Jenny; Wenk, Markus; Di Paolo, Gilbert; Nemoto, Yasuo; Crum, John; Ellisman, Mark H.; De Camilli, Pietro; Shupliakov, Oleg; Brodin, Lennart.

In: Neuron, Vol. 27, No. 2, 01.01.2000, p. 301-312.

Research output: Contribution to journal › Article › peer-review

Harvard

Gad, H, Ringstad, N, Löw, P, Kjaerulff, O, Gustafsson, J, Wenk, M, Di Paolo, G, Nemoto, Y, Crum, J, Ellisman, MH, De Camilli, P, Shupliakov, O & Brodin, L 2000, 'Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin', Neuron, vol. 27, no. 2, pp. 301-312. https://doi.org/10.1016/S0896-6273(00)00038-6

APA

Gad, H., Ringstad, N., Löw, P., Kjaerulff, O., Gustafsson, J., Wenk, M., Di Paolo, G., Nemoto, Y., Crum, J., Ellisman, M. H., De Camilli, P., Shupliakov, O., & Brodin, L. (2000). Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin. Neuron, 27(2), 301-312. https://doi.org/10.1016/S0896-6273(00)00038-6

Vancouver

Gad H, Ringstad N, Löw P, Kjaerulff O, Gustafsson J, Wenk M et al. Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin. Neuron. 2000 Jan 1;27(2):301-312. https://doi.org/10.1016/S0896-6273(00)00038-6

Author

Gad, Helge ; Ringstad, Niels ; Löw, Peter ; Kjaerulff, Ole ; Gustafsson, Jenny ; Wenk, Markus ; Di Paolo, Gilbert ; Nemoto, Yasuo ; Crum, John ; Ellisman, Mark H. ; De Camilli, Pietro ; Shupliakov, Oleg ; Brodin, Lennart. / Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin. In: Neuron. 2000 ; Vol. 27, No. 2. pp. 301-312.

BibTeX

@article{a711749dd9a343e294ffdb8aee86d9e5,
title = "Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin",
abstract = "Coordination between sequential steps in synaptic vesicle endocytosis, including clathrin coat formation, fission, and uncoating, appears to involve proteinprotein interactions. Here, we show that compounds that disrupt interactions of the SH3 domain of endophilin with dynamin and synaptojanin impair synaptic vesicle endocytosis in a living synapse. Two distinct endocytic intermediates accumulated. Free clathrincoated vesicles were induced by a peptide-blocking endophilin's SH3 domain and by antibodies to the proline-rich domain (PRD) of synaptojanin. Invaginated clathrin-coated pits were induced by the same peptide and by the SH3 domain of endophilin. We suggest that the SH3 domain of endophilin participates in both fission and uncoating and that it may be a key component of a molecular switch that couples the fission reaction to uncoating.",
author = "Helge Gad and Niels Ringstad and Peter L{\"o}w and Ole Kjaerulff and Jenny Gustafsson and Markus Wenk and {Di Paolo}, Gilbert and Yasuo Nemoto and John Crum and Ellisman, {Mark H.} and {De Camilli}, Pietro and Oleg Shupliakov and Lennart Brodin",
year = "2000",
month = jan,
day = "1",
doi = "10.1016/S0896-6273(00)00038-6",
language = "English",
volume = "27",
pages = "301--312",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Fission and uncoating of synaptic clathrin-coated vesicles are perturbed by disruption of interactions with the SH3 domain of endophilin

AU - Gad, Helge

AU - Ringstad, Niels

AU - Löw, Peter

AU - Kjaerulff, Ole

AU - Gustafsson, Jenny

AU - Wenk, Markus

AU - Di Paolo, Gilbert

AU - Nemoto, Yasuo

AU - Crum, John

AU - Ellisman, Mark H.

AU - De Camilli, Pietro

AU - Shupliakov, Oleg

AU - Brodin, Lennart

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Coordination between sequential steps in synaptic vesicle endocytosis, including clathrin coat formation, fission, and uncoating, appears to involve proteinprotein interactions. Here, we show that compounds that disrupt interactions of the SH3 domain of endophilin with dynamin and synaptojanin impair synaptic vesicle endocytosis in a living synapse. Two distinct endocytic intermediates accumulated. Free clathrincoated vesicles were induced by a peptide-blocking endophilin's SH3 domain and by antibodies to the proline-rich domain (PRD) of synaptojanin. Invaginated clathrin-coated pits were induced by the same peptide and by the SH3 domain of endophilin. We suggest that the SH3 domain of endophilin participates in both fission and uncoating and that it may be a key component of a molecular switch that couples the fission reaction to uncoating.

AB - Coordination between sequential steps in synaptic vesicle endocytosis, including clathrin coat formation, fission, and uncoating, appears to involve proteinprotein interactions. Here, we show that compounds that disrupt interactions of the SH3 domain of endophilin with dynamin and synaptojanin impair synaptic vesicle endocytosis in a living synapse. Two distinct endocytic intermediates accumulated. Free clathrincoated vesicles were induced by a peptide-blocking endophilin's SH3 domain and by antibodies to the proline-rich domain (PRD) of synaptojanin. Invaginated clathrin-coated pits were induced by the same peptide and by the SH3 domain of endophilin. We suggest that the SH3 domain of endophilin participates in both fission and uncoating and that it may be a key component of a molecular switch that couples the fission reaction to uncoating.

UR - http://www.scopus.com/inward/record.url?scp=0033678935&partnerID=8YFLogxK

U2 - 10.1016/S0896-6273(00)00038-6

DO - 10.1016/S0896-6273(00)00038-6

M3 - Article

C2 - 10985350

AN - SCOPUS:0033678935

VL - 27

SP - 301

EP - 312

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 2

ER -

ID: 40834662