FISH on avian lampbrush chromosomes produces higher resolution gene mapping

Standard

FISH on avian lampbrush chromosomes produces higher resolution gene mapping. / Galkina, Svetlana; Deryusheva, Svetlana; Fillon, Valerie; Vignal, Alain; Crooijmans, Richard; Groenen, Martin; Rodionov, Alexander ; Gaginskaya, Elena.

In: Genetica, Vol. 128, No. 1-3, 01.09.2006, p. 241-251.

Research output: Contribution to journal › Article › peer-review

Author

Galkina, Svetlana ; Deryusheva, Svetlana ; Fillon, Valerie ; Vignal, Alain ; Crooijmans, Richard ; Groenen, Martin ; Rodionov, Alexander ; Gaginskaya, Elena. / FISH on avian lampbrush chromosomes produces higher resolution gene mapping. In: Genetica. 2006 ; Vol. 128, No. 1-3. pp. 241-251.

BibTeX

@article{833ecfd192e4454d8a726f7355dc53b8,

title = "FISH on avian lampbrush chromosomes produces higher resolution gene mapping",

abstract = "Giant lampbrush chromosomes, which are characteristic of the diplotene stage of prophase I during avian oogenesis, represent a very promising system for precise physical gene mapping. We applied 35 chicken BAC and 4 PAC clones to both mitotic metaphase chromosomes and meiotic lampbrush chromosomes of chicken (Gallus gallus domesticus) and Japanese quail (Coturnix coturnix japonica). Fluorescence in situ hybridization (FISH) mapping on lampbrush chromosomes allowed us to distinguish closely located probes and revealed gene order more precisely. Our data extended the data earlier obtained using FISH to chicken and quail metaphase chromosomes 1-6 and Z. Extremely low levels of inter- and intra-chromosomal rearrangements in the chicken and Japanese quail were demonstrated again. Moreover, we did not confirm the presence of a pericentric inversion in Japanese quail chromosome 4 as compared to chicken chromosome 4. Twelve BAC clones specific for chicken chromosome 4p and 4q showed the same order in quail as in chicken when FISH was performed on lampbrush chromosomes. The centromeres of chicken and quail chromosomes 4 seem to have formed independently after centric fusion of ancestral chromosome 4 and a microchromosome.",

keywords = "BAC clone, Chicken, Chromosome rearrangement, FISH mapping, Japanese quail, Lampbrush chromosome",

author = "Svetlana Galkina and Svetlana Deryusheva and Valerie Fillon and Alain Vignal and Richard Crooijmans and Martin Groenen and Alexander Rodionov and Elena Gaginskaya",

year = "2006",

month = sep,

day = "1",

doi = "10.1007/s10709-005-5776-7",

language = "English",

volume = "128",

pages = "241--251",

journal = "Genetica",

issn = "0016-6707",

publisher = "Springer Nature",

number = "1-3",

}

RIS

TY - JOUR

T1 - FISH on avian lampbrush chromosomes produces higher resolution gene mapping

AU - Galkina, Svetlana

AU - Deryusheva, Svetlana

AU - Fillon, Valerie

AU - Vignal, Alain

AU - Crooijmans, Richard

AU - Groenen, Martin

AU - Rodionov, Alexander

AU - Gaginskaya, Elena

PY - 2006/9/1

Y1 - 2006/9/1

N2 - Giant lampbrush chromosomes, which are characteristic of the diplotene stage of prophase I during avian oogenesis, represent a very promising system for precise physical gene mapping. We applied 35 chicken BAC and 4 PAC clones to both mitotic metaphase chromosomes and meiotic lampbrush chromosomes of chicken (Gallus gallus domesticus) and Japanese quail (Coturnix coturnix japonica). Fluorescence in situ hybridization (FISH) mapping on lampbrush chromosomes allowed us to distinguish closely located probes and revealed gene order more precisely. Our data extended the data earlier obtained using FISH to chicken and quail metaphase chromosomes 1-6 and Z. Extremely low levels of inter- and intra-chromosomal rearrangements in the chicken and Japanese quail were demonstrated again. Moreover, we did not confirm the presence of a pericentric inversion in Japanese quail chromosome 4 as compared to chicken chromosome 4. Twelve BAC clones specific for chicken chromosome 4p and 4q showed the same order in quail as in chicken when FISH was performed on lampbrush chromosomes. The centromeres of chicken and quail chromosomes 4 seem to have formed independently after centric fusion of ancestral chromosome 4 and a microchromosome.

AB - Giant lampbrush chromosomes, which are characteristic of the diplotene stage of prophase I during avian oogenesis, represent a very promising system for precise physical gene mapping. We applied 35 chicken BAC and 4 PAC clones to both mitotic metaphase chromosomes and meiotic lampbrush chromosomes of chicken (Gallus gallus domesticus) and Japanese quail (Coturnix coturnix japonica). Fluorescence in situ hybridization (FISH) mapping on lampbrush chromosomes allowed us to distinguish closely located probes and revealed gene order more precisely. Our data extended the data earlier obtained using FISH to chicken and quail metaphase chromosomes 1-6 and Z. Extremely low levels of inter- and intra-chromosomal rearrangements in the chicken and Japanese quail were demonstrated again. Moreover, we did not confirm the presence of a pericentric inversion in Japanese quail chromosome 4 as compared to chicken chromosome 4. Twelve BAC clones specific for chicken chromosome 4p and 4q showed the same order in quail as in chicken when FISH was performed on lampbrush chromosomes. The centromeres of chicken and quail chromosomes 4 seem to have formed independently after centric fusion of ancestral chromosome 4 and a microchromosome.

KW - BAC clone

KW - Chicken

KW - Chromosome rearrangement

KW - FISH mapping

KW - Japanese quail

KW - Lampbrush chromosome

UR - http://www.scopus.com/inward/record.url?scp=33749608825&partnerID=8YFLogxK

U2 - 10.1007/s10709-005-5776-7

DO - 10.1007/s10709-005-5776-7

M3 - Article

C2 - 17028954

AN - SCOPUS:33749608825

VL - 128

SP - 241

EP - 251

JO - Genetica

JF - Genetica

SN - 0016-6707

IS - 1-3

ER -

ID: 36720359