First insights into the gut microbiomes and the diet of the Littorina snail ecotypes, a recently emerged marine evolutionary model

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First insights into the gut microbiomes and the diet of the Littorina snail ecotypes, a recently emerged marine evolutionary model. / Panova, Marina A.Z.; Varfolomeeva, Marina A.; Gafarova, Elizaveta R.; Maltseva, Arina L.; Mikhailova, Natalia A.; Granovitch, Andrei I.

In: Evolutionary Applications, 13.07.2022.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{aec7b65fba704674938ea20feef0de84,

title = "First insights into the gut microbiomes and the diet of the Littorina snail ecotypes, a recently emerged marine evolutionary model",

abstract = "Microbes can play a prominent role in the evolution of their hosts, facilitating adaptation to various environments and promoting ecological divergence. The Wave and Crab ecotypes of the intertidal snail Littorina saxatilis is an evolutionary model of rapid and repeated adaptation to environmental gradients. While patterns of genomic divergence of the Littorina ecotypes along the shore gradients have been extensively studied, their microbiomes have been so far overlooked. The aim of the present study is to start filling this gap by comparing gut microbiome composition of the Wave and Crab ecotypes using metabarcoding approach. Since Littorina snails are micro-grazers feeding on the intertidal biofilm, we also compare biofilm composition (i.e. typical snail diet) in the crab and wave habitats. In the results, we found that bacterial and eukaryotic biofilm composition varies between the typical habitats of the ecotypes. Further, the snail gut bacteriome was different from outer environments, being dominated by Gammaproteobacteria, Fusobacteria, Bacteroidia and Alphaproteobacteria. There were clear differences in the gut bacterial communities between the Crab and the Wave ecotypes as well as between the Wave ecotype snails from the low and high shores. These differences were both observed in the abundances and in the presence of different bacteria, as well as at different taxonomic level, from bacterial OTU's to families. Altogether, our first insights show that Littorina snails and their associated bacteria are a promising marine system to study co-evolution of the microbes and their hosts, which can help us to predict the future for wild species in the face of rapidly changing marine environments.",

keywords = "ecological divergence, host-microbiome interaction, intertidal, Littorina, microbiome",

author = "Panova, {Marina A.Z.} and Varfolomeeva, {Marina A.} and Gafarova, {Elizaveta R.} and Maltseva, {Arina L.} and Mikhailova, {Natalia A.} and Granovitch, {Andrei I.}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Evolutionary Applications published by John Wiley & Sons Ltd.",

year = "2022",

month = jul,

day = "13",

doi = "10.1111/eva.13447",

language = "English",

journal = "Evolutionary Applications",

issn = "1752-4571",

publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - First insights into the gut microbiomes and the diet of the Littorina snail ecotypes, a recently emerged marine evolutionary model

AU - Panova, Marina A.Z.

AU - Varfolomeeva, Marina A.

AU - Gafarova, Elizaveta R.

AU - Maltseva, Arina L.

AU - Mikhailova, Natalia A.

AU - Granovitch, Andrei I.

PY - 2022/7/13

Y1 - 2022/7/13

N2 - Microbes can play a prominent role in the evolution of their hosts, facilitating adaptation to various environments and promoting ecological divergence. The Wave and Crab ecotypes of the intertidal snail Littorina saxatilis is an evolutionary model of rapid and repeated adaptation to environmental gradients. While patterns of genomic divergence of the Littorina ecotypes along the shore gradients have been extensively studied, their microbiomes have been so far overlooked. The aim of the present study is to start filling this gap by comparing gut microbiome composition of the Wave and Crab ecotypes using metabarcoding approach. Since Littorina snails are micro-grazers feeding on the intertidal biofilm, we also compare biofilm composition (i.e. typical snail diet) in the crab and wave habitats. In the results, we found that bacterial and eukaryotic biofilm composition varies between the typical habitats of the ecotypes. Further, the snail gut bacteriome was different from outer environments, being dominated by Gammaproteobacteria, Fusobacteria, Bacteroidia and Alphaproteobacteria. There were clear differences in the gut bacterial communities between the Crab and the Wave ecotypes as well as between the Wave ecotype snails from the low and high shores. These differences were both observed in the abundances and in the presence of different bacteria, as well as at different taxonomic level, from bacterial OTU's to families. Altogether, our first insights show that Littorina snails and their associated bacteria are a promising marine system to study co-evolution of the microbes and their hosts, which can help us to predict the future for wild species in the face of rapidly changing marine environments.

AB - Microbes can play a prominent role in the evolution of their hosts, facilitating adaptation to various environments and promoting ecological divergence. The Wave and Crab ecotypes of the intertidal snail Littorina saxatilis is an evolutionary model of rapid and repeated adaptation to environmental gradients. While patterns of genomic divergence of the Littorina ecotypes along the shore gradients have been extensively studied, their microbiomes have been so far overlooked. The aim of the present study is to start filling this gap by comparing gut microbiome composition of the Wave and Crab ecotypes using metabarcoding approach. Since Littorina snails are micro-grazers feeding on the intertidal biofilm, we also compare biofilm composition (i.e. typical snail diet) in the crab and wave habitats. In the results, we found that bacterial and eukaryotic biofilm composition varies between the typical habitats of the ecotypes. Further, the snail gut bacteriome was different from outer environments, being dominated by Gammaproteobacteria, Fusobacteria, Bacteroidia and Alphaproteobacteria. There were clear differences in the gut bacterial communities between the Crab and the Wave ecotypes as well as between the Wave ecotype snails from the low and high shores. These differences were both observed in the abundances and in the presence of different bacteria, as well as at different taxonomic level, from bacterial OTU's to families. Altogether, our first insights show that Littorina snails and their associated bacteria are a promising marine system to study co-evolution of the microbes and their hosts, which can help us to predict the future for wild species in the face of rapidly changing marine environments.

KW - ecological divergence

KW - host-microbiome interaction

KW - intertidal

KW - Littorina

KW - microbiome

UR - http://www.scopus.com/inward/record.url?scp=85134568063&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/62c87b1e-74dd-3eea-beed-8ea09def3595/

U2 - 10.1111/eva.13447

DO - 10.1111/eva.13447

M3 - Article

AN - SCOPUS:85134568063

JO - Evolutionary Applications

JF - Evolutionary Applications

SN - 1752-4571

ER -

ID: 100967403