The genital pore of the female of Intoshia variabili Aleksandrov et Sljusarev, 1992 is located on a transverse ring of non-ciliated cells. Before copulation it is occupied by 6-7 cells that develop in the epithelium and that for a time are beneath the surface. When fully developed, these cells contain many electron-dense granules and their apical surface is covered with numerous microvilli. After copulation the granules disappear and the apical surface becomes smooth. The cells still form a plug closing the opening. After one larva pushes the pore cells out and escapes, others follow.