Fibrillar Actin in Nuclear Apparatus of Ciliate Paramecium Caudatum

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Fibrillar Actin in Nuclear Apparatus of Ciliate Paramecium Caudatum. / Benken, K. A.; Sabaneyeva, E. V.

In: Cell and Tissue Biology, Vol. 5, No. 5, 2011, p. 471-479.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{6d229ac985a24da49ec5161712ebcb26,

title = "Fibrillar Actin in Nuclear Apparatus of Ciliate Paramecium Caudatum",

abstract = "Ciliates are a good model for studying the role of actin in the nuclear spatial organization and transcription activity due to their nuclear dualism. Actin localization in the nuclear apparatus of the ciliate Paramecium caudatum was studied using fluorescently labeled phalloidin and indirect immunocytochemistry. Fibrillar actin was visualized in both nuclei. Actin was revealed in areas of the chromatin that are often associated with the periphery of the amplified nucleoli in the macronucleus. Actin distribution depended on the cell physiological state. The infection of the macronucleus with the intranuclear endobiont Holospora obtusaresulted in a loss of nuclear actin, nuclear fragility, and redistribution of the phosphorylated form of the actin binding protein cofilin. A spherical body that resembles the karyosphere was found in the macronuclear anlagen.",

keywords = "nuclear actin, ciliates, nuclear dualism, macronucleus, amplified nucleoli, cofilin, intranuclear endobionts",

author = "Benken, {K. A.} and Sabaneyeva, {E. V.}",

year = "2011",

doi = "DOI: 10.1134/S1990519X11050026",

language = "English",

volume = "5",

pages = "471--479",

journal = "Cell and Tissue Biology",

issn = "1990-519X",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "5",

}

RIS

TY - JOUR

T1 - Fibrillar Actin in Nuclear Apparatus of Ciliate Paramecium Caudatum

AU - Benken, K. A.

AU - Sabaneyeva, E. V.

PY - 2011

Y1 - 2011

N2 - Ciliates are a good model for studying the role of actin in the nuclear spatial organization and transcription activity due to their nuclear dualism. Actin localization in the nuclear apparatus of the ciliate Paramecium caudatum was studied using fluorescently labeled phalloidin and indirect immunocytochemistry. Fibrillar actin was visualized in both nuclei. Actin was revealed in areas of the chromatin that are often associated with the periphery of the amplified nucleoli in the macronucleus. Actin distribution depended on the cell physiological state. The infection of the macronucleus with the intranuclear endobiont Holospora obtusaresulted in a loss of nuclear actin, nuclear fragility, and redistribution of the phosphorylated form of the actin binding protein cofilin. A spherical body that resembles the karyosphere was found in the macronuclear anlagen.

AB - Ciliates are a good model for studying the role of actin in the nuclear spatial organization and transcription activity due to their nuclear dualism. Actin localization in the nuclear apparatus of the ciliate Paramecium caudatum was studied using fluorescently labeled phalloidin and indirect immunocytochemistry. Fibrillar actin was visualized in both nuclei. Actin was revealed in areas of the chromatin that are often associated with the periphery of the amplified nucleoli in the macronucleus. Actin distribution depended on the cell physiological state. The infection of the macronucleus with the intranuclear endobiont Holospora obtusaresulted in a loss of nuclear actin, nuclear fragility, and redistribution of the phosphorylated form of the actin binding protein cofilin. A spherical body that resembles the karyosphere was found in the macronuclear anlagen.

KW - nuclear actin

KW - ciliates

KW - nuclear dualism

KW - macronucleus

KW - amplified nucleoli

KW - cofilin

KW - intranuclear endobionts

U2 - DOI: 10.1134/S1990519X11050026

DO - DOI: 10.1134/S1990519X11050026

M3 - Article

VL - 5

SP - 471

EP - 479

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 5

ER -

ID: 5154415