Research output: Contribution to journal › Article › peer-review
Factors of early protective action of live influenza vaccine combined with recombinant bacterial polypeptides against homologous and heterologous influenza infection. / Desheva, Yulia A.; Leontieva, Galina F.; Kramskaya, Tatiana A.; Landgraf, Galina O.; Sychev, Ivan A.; Rekstin, Andrey R.; Suvorov, Alexander N.
In: Heliyon, Vol. 5, No. 2, e01154, 01.02.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Factors of early protective action of live influenza vaccine combined with recombinant bacterial polypeptides against homologous and heterologous influenza infection
AU - Desheva, Yulia A.
AU - Leontieva, Galina F.
AU - Kramskaya, Tatiana A.
AU - Landgraf, Galina O.
AU - Sychev, Ivan A.
AU - Rekstin, Andrey R.
AU - Suvorov, Alexander N.
PY - 2019/2/1
Y1 - 2019/2/1
N2 - We are developing an associated vaccine based on live influenza vaccine (LAIV) and streptococcal recombinant peptides. The recombinant group B streptococcus (GBS) peptides P6 and ScaAB demonstrated a distinguished immunomodulating effect in THP-1 cells. The increase in IFN 1-alpha expression after ScaAB inoculation was similar to that against LAIV. We immunized mice intranasal using of A/H7N3 LAIV or/and ScaAB peptide. At day 5 after immunization, we detected serum IgM which reacted with non-vaccine influenza viruses. Associated vaccination of mice using LAIV and GBS peptide was the most effective against sub-lethal infection with A/H7N9 influenza virus and against lethal challenge with A/H1N1pdm virus at day 5 after immunization. Not only LAIV but also the ScaAB protected about 20% of the immunized animals against lethal challenge with A/H1N1pdm virus. The early protection was related to increasing type 1 interferons expression in the lungs. Our results in mice have shown that successful protection against homologous and heterologous influenza infections can be achieved soon after vaccination with either LAIV or LAIV in combination with GBS recombinant peptide. Presumably, such protection may be mediated by non-specific IgM antibodies and an increase in the expression of early cytokines in the airway.
AB - We are developing an associated vaccine based on live influenza vaccine (LAIV) and streptococcal recombinant peptides. The recombinant group B streptococcus (GBS) peptides P6 and ScaAB demonstrated a distinguished immunomodulating effect in THP-1 cells. The increase in IFN 1-alpha expression after ScaAB inoculation was similar to that against LAIV. We immunized mice intranasal using of A/H7N3 LAIV or/and ScaAB peptide. At day 5 after immunization, we detected serum IgM which reacted with non-vaccine influenza viruses. Associated vaccination of mice using LAIV and GBS peptide was the most effective against sub-lethal infection with A/H7N9 influenza virus and against lethal challenge with A/H1N1pdm virus at day 5 after immunization. Not only LAIV but also the ScaAB protected about 20% of the immunized animals against lethal challenge with A/H1N1pdm virus. The early protection was related to increasing type 1 interferons expression in the lungs. Our results in mice have shown that successful protection against homologous and heterologous influenza infections can be achieved soon after vaccination with either LAIV or LAIV in combination with GBS recombinant peptide. Presumably, such protection may be mediated by non-specific IgM antibodies and an increase in the expression of early cytokines in the airway.
KW - Immunology
KW - Microbiology
KW - Vaccines
KW - Virology
KW - PATHOGENESIS
KW - IMMUNE-RESPONSE
KW - CELLS
KW - VIRUS
KW - PROTEIN
KW - I INTERFERONS
KW - BETA
UR - http://www.scopus.com/inward/record.url?scp=85060994853&partnerID=8YFLogxK
U2 - 10.1016/j.heliyon.2019.e01154
DO - 10.1016/j.heliyon.2019.e01154
M3 - Article
AN - SCOPUS:85060994853
VL - 5
JO - Heliyon
JF - Heliyon
SN - 2405-8440
IS - 2
M1 - e01154
ER -
ID: 39067358