This paper presents a new program F2breed for Windows and Linux operating systems, which was designed for estimation of recombination frequency between the genetic loci and construction of genetic maps based on the analysis of inheritance in a F2 population. The program implements the approach of the orientation of n points corresponding to n analyzed loci in the (n–1)-dimensional space for relative arrangement of the loci on the genetic map by consideration of various projections of this structure. The program is characterized by a convenient, intuitive interface, is easy to operate, and is suitable for working with small groups of loci (up to 100).