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Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis. / Antelmann, Haike; Engelmann, Susanne; Schmid, Roland; Sorokin, Alexei; Lapidus, Alla; Hecker, Michael.

In: Journal of Bacteriology, Vol. 179, No. 23, 12.1997, p. 7251-7256.

Research output: Contribution to journal › Article › peer-review

Harvard

Antelmann, H, Engelmann, S, Schmid, R, Sorokin, A, Lapidus, A & Hecker, M 1997, 'Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis', Journal of Bacteriology, vol. 179, no. 23, pp. 7251-7256. https://doi.org/10.1128/jb.179.23.7251-7256.1997

APA

Antelmann, H., Engelmann, S., Schmid, R., Sorokin, A., Lapidus, A., & Hecker, M. (1997). Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis. Journal of Bacteriology, 179(23), 7251-7256. https://doi.org/10.1128/jb.179.23.7251-7256.1997

Vancouver

Antelmann H, Engelmann S, Schmid R, Sorokin A, Lapidus A, Hecker M. Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis. Journal of Bacteriology. 1997 Dec;179(23):7251-7256. https://doi.org/10.1128/jb.179.23.7251-7256.1997

Author

Antelmann, Haike ; Engelmann, Susanne ; Schmid, Roland ; Sorokin, Alexei ; Lapidus, Alla ; Hecker, Michael. / Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis. In: Journal of Bacteriology. 1997 ; Vol. 179, No. 23. pp. 7251-7256.

BibTeX

@article{7adc5d80254d4f85ba99fc0897c30511,
title = "Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis",
abstract = "σ(B)-dependent general stress proteins (Gsps) of Bacillus subtilis are essential for the development of glucose-starvation-induced cross-resistance to oxidative challenge. However, the proteins directly involved in this nonspecific resistance to oxidative stress have to be identified. We found that one prominent Gsp displayed strong sequence similarity to the previously characterized oxidative-stress-inducible MrgA protein of B. subtilis and to the starvation-induced Dps/PexB protein of Escherichia coli. We therefore designated this prominent Gsp Dps. While MrgA belongs to the peroxide- stress-inducible proteins needed for the H2O2-inducible adaptive response to oxidative stress, Dps belongs to the proteins induced by heat, salt, or ethanol stress and after starvation for glucose but not by a sublethal oxidative challenge. Primer extension experiments identified two overlapping promoters upstream of the coding region of dps, one being σ(B) dependent (P(B)) and the other being σ(B) independent (P1). Both promoters contribute to the basal level of dps during growth. After stress or during entry into the stationary phase, transcription from P1 strongly increased whereas transcription from P1 decreased. Mutant strains lacking Dps completely failed to develop glucose-starvation-induced resistance to oxidative stress. These results confirm our suggestion that σ(B)-dependent general stress proteins of B. subtilis are absolutely required for the development of nonspecific resistance to oxidative stress.",
author = "Haike Antelmann and Susanne Engelmann and Roland Schmid and Alexei Sorokin and Alla Lapidus and Michael Hecker",
year = "1997",
month = dec,
doi = "10.1128/jb.179.23.7251-7256.1997",
language = "English",
volume = "179",
pages = "7251--7256",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "23",

}

RIS

TY - JOUR

T1 - Expression of a stress- and starvation-induced dps/pexB-homologous gene is controlled by the alternative sigma factor σ(B) in Bacillus subtilis

AU - Antelmann, Haike

AU - Engelmann, Susanne

AU - Schmid, Roland

AU - Sorokin, Alexei

AU - Lapidus, Alla

AU - Hecker, Michael

PY - 1997/12

Y1 - 1997/12

N2 - σ(B)-dependent general stress proteins (Gsps) of Bacillus subtilis are essential for the development of glucose-starvation-induced cross-resistance to oxidative challenge. However, the proteins directly involved in this nonspecific resistance to oxidative stress have to be identified. We found that one prominent Gsp displayed strong sequence similarity to the previously characterized oxidative-stress-inducible MrgA protein of B. subtilis and to the starvation-induced Dps/PexB protein of Escherichia coli. We therefore designated this prominent Gsp Dps. While MrgA belongs to the peroxide- stress-inducible proteins needed for the H2O2-inducible adaptive response to oxidative stress, Dps belongs to the proteins induced by heat, salt, or ethanol stress and after starvation for glucose but not by a sublethal oxidative challenge. Primer extension experiments identified two overlapping promoters upstream of the coding region of dps, one being σ(B) dependent (P(B)) and the other being σ(B) independent (P1). Both promoters contribute to the basal level of dps during growth. After stress or during entry into the stationary phase, transcription from P1 strongly increased whereas transcription from P1 decreased. Mutant strains lacking Dps completely failed to develop glucose-starvation-induced resistance to oxidative stress. These results confirm our suggestion that σ(B)-dependent general stress proteins of B. subtilis are absolutely required for the development of nonspecific resistance to oxidative stress.

AB - σ(B)-dependent general stress proteins (Gsps) of Bacillus subtilis are essential for the development of glucose-starvation-induced cross-resistance to oxidative challenge. However, the proteins directly involved in this nonspecific resistance to oxidative stress have to be identified. We found that one prominent Gsp displayed strong sequence similarity to the previously characterized oxidative-stress-inducible MrgA protein of B. subtilis and to the starvation-induced Dps/PexB protein of Escherichia coli. We therefore designated this prominent Gsp Dps. While MrgA belongs to the peroxide- stress-inducible proteins needed for the H2O2-inducible adaptive response to oxidative stress, Dps belongs to the proteins induced by heat, salt, or ethanol stress and after starvation for glucose but not by a sublethal oxidative challenge. Primer extension experiments identified two overlapping promoters upstream of the coding region of dps, one being σ(B) dependent (P(B)) and the other being σ(B) independent (P1). Both promoters contribute to the basal level of dps during growth. After stress or during entry into the stationary phase, transcription from P1 strongly increased whereas transcription from P1 decreased. Mutant strains lacking Dps completely failed to develop glucose-starvation-induced resistance to oxidative stress. These results confirm our suggestion that σ(B)-dependent general stress proteins of B. subtilis are absolutely required for the development of nonspecific resistance to oxidative stress.

UR - http://www.scopus.com/inward/record.url?scp=0030665283&partnerID=8YFLogxK

U2 - 10.1128/jb.179.23.7251-7256.1997

DO - 10.1128/jb.179.23.7251-7256.1997

M3 - Article

C2 - 9393687

AN - SCOPUS:0030665283

VL - 179

SP - 7251

EP - 7256

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 23

ER -

ID: 90042981