Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing. / Krutikova, E. V.; Stepanova, E. A.; Kiseleva, I. V.; Rudenko, L. G.
In: Molecular Genetics, Microbiology and Virology, Vol. 33, No. 2, 01.04.2018, p. 139-144.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Express Screening of Reassortants of Influenza Virus B—Candidates for Vaccinating Strains of Live Influenza Vaccine by Using Pyrosequencing
AU - Krutikova, E. V.
AU - Stepanova, E. A.
AU - Kiseleva, I. V.
AU - Rudenko, L. G.
N1 - Funding Information: ACKNOWLEDGMENTS This work was supported by grant no. 14-15-00034 of the Russian Science Foundation (Moscow, Russia). Publisher Copyright: © 2018, Allerton Press, Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Abstract: For the rapid preparation of strains of live influenza vaccine, it is important to have a rapid method for screening vaccine reassortants. In this paper, a method of pyrosequencing was used to analyze the genome of reassortants obtained by crossing the donor of attenuation B/Leningrad/14/17/55 and the wild-type virus B/Indiana/25/2015. It has been shown that pyrosequencing allows the composition of the reassortant genome to be evaluated quickly, economically, and with high accuracy.
AB - Abstract: For the rapid preparation of strains of live influenza vaccine, it is important to have a rapid method for screening vaccine reassortants. In this paper, a method of pyrosequencing was used to analyze the genome of reassortants obtained by crossing the donor of attenuation B/Leningrad/14/17/55 and the wild-type virus B/Indiana/25/2015. It has been shown that pyrosequencing allows the composition of the reassortant genome to be evaluated quickly, economically, and with high accuracy.
KW - genome analysis
KW - influenza virus
KW - live reassortant influenza vaccine
KW - pyrosequencing
UR - http://www.scopus.com/inward/record.url?scp=85058029204&partnerID=8YFLogxK
U2 - 10.3103/S089141681802009X
DO - 10.3103/S089141681802009X
M3 - Article
AN - SCOPUS:85058029204
VL - 33
SP - 139
EP - 144
JO - Molecular Genetics, Microbiology and Virology
JF - Molecular Genetics, Microbiology and Virology
SN - 0891-4168
IS - 2
ER -
ID: 75082816