TY - JOUR

T1 - Exploring the dual impact of hydrocarbon chainlength and the role of piroxicam a conventional NSAID on soylecithin/ion pair amphiphiles mediated hybrid vesicles for brain – tumor targeted drug delivery

AU - Guha, Pritam

AU - Roy, Biplab

AU - Nahak, Prasant

AU - Karmakar, Gourab

AU - Chang, Chien H.

AU - Быков, Алексей Геннадьевич

AU - Акентьев, Александр Владимирович

AU - Носков, Борис Анатольевич

AU - Mandal, Amit Kumar

AU - Kumar, Anoop

AU - Hassan, P.A.

AU - Aswal, V.K.

AU - Misono, Takeshi

AU - Torigoe, Kanjiro

AU - Panda, Amiya Kumar

N1 - Funding Information: The research project is funded by Council of Scientific & Industrial Research (Project No.-01(2533)/11/EMR-II), India. P.G. sincerely acknowledges University Grants Commission (UGC) – Basic Scientific Research (BSR) for a research fellowship. Appendix A

PY - 2018/6/5

Y1 - 2018/6/5

N2 - Development of drug delivery systems, not the drug discovery, has become more cynosures towards the efficacy of drug against the target cells. Modified hybrid cationic vesicles (HCV) were formulated using soylecithin (SLC), ion pair amphiphile (IPA, hexadecyltrimethylammonium-dodecylsulfate, HTMA+-DS−), bi-tail cationic surfactant dialkyldimethylammonium bromides (DXDABs, bis-C12 to C18) and cholesterol. Piroxicam (Px), a conventional non steroidal anti inflamatory drug (NSAID), with potent yet unexplored anticancer activity, was encapsulated in the hybrid vesicles. Dual impact of DXDABs and Px on SLC/IPA were scrutinized in the form of monolayer, bilayer and solid supported bilayer. Favourable hydrophobic inteaction between SLC/IPA anddihexadecyldimethylammonium bromide (DHDAB) as well as the intercalation of Px molecules between the amphiphiles were noticed through the surface pressure area measuremnts. Vesicles without and with Px were fairly monodisperse with positive zeta potential (Z. P.) and considerably stable upto two months. Size of the vesicles enhanced with Px incorporation. Vesicles maintain spherical morphology as revealed from the electronic microscopic studies. Differential scanning calaorimetry and FTIR studies confirm the location of Px membrane palisade that enhances the extent of hydration by increasing the proportion of H-bonding. Bilayer thickness and the spacing between two adjecent lamellar phase were investigated by combined small angle neutron scattering and small angle X-ray scattering. Atomic force microscopic studies confirm the Px induced fluidization of membrane bilayer. The entrapment efficiency of vesicles to host Px depends on the amount of IPA present in the bilayer. Px hosted cationic vesicles showed less than 2% hemolysis. The drug reigned supreme over human neuroblastoma cell line (SH-SY 5Y) when encapsulated inside the membrane and was non toxic to normal human blood cell lymphocyte (PBMC) as revealed from cytotoxicity assay.

AB - Development of drug delivery systems, not the drug discovery, has become more cynosures towards the efficacy of drug against the target cells. Modified hybrid cationic vesicles (HCV) were formulated using soylecithin (SLC), ion pair amphiphile (IPA, hexadecyltrimethylammonium-dodecylsulfate, HTMA+-DS−), bi-tail cationic surfactant dialkyldimethylammonium bromides (DXDABs, bis-C12 to C18) and cholesterol. Piroxicam (Px), a conventional non steroidal anti inflamatory drug (NSAID), with potent yet unexplored anticancer activity, was encapsulated in the hybrid vesicles. Dual impact of DXDABs and Px on SLC/IPA were scrutinized in the form of monolayer, bilayer and solid supported bilayer. Favourable hydrophobic inteaction between SLC/IPA anddihexadecyldimethylammonium bromide (DHDAB) as well as the intercalation of Px molecules between the amphiphiles were noticed through the surface pressure area measuremnts. Vesicles without and with Px were fairly monodisperse with positive zeta potential (Z. P.) and considerably stable upto two months. Size of the vesicles enhanced with Px incorporation. Vesicles maintain spherical morphology as revealed from the electronic microscopic studies. Differential scanning calaorimetry and FTIR studies confirm the location of Px membrane palisade that enhances the extent of hydration by increasing the proportion of H-bonding. Bilayer thickness and the spacing between two adjecent lamellar phase were investigated by combined small angle neutron scattering and small angle X-ray scattering. Atomic force microscopic studies confirm the Px induced fluidization of membrane bilayer. The entrapment efficiency of vesicles to host Px depends on the amount of IPA present in the bilayer. Px hosted cationic vesicles showed less than 2% hemolysis. The drug reigned supreme over human neuroblastoma cell line (SH-SY 5Y) when encapsulated inside the membrane and was non toxic to normal human blood cell lymphocyte (PBMC) as revealed from cytotoxicity assay.

KW - ANGLE NEUTRON-SCATTERING; X-RAY-SCATTERING; BILAYER THICKNESS; PHOSPHATIDYLCHOLINE VESICLES; CATIONIC SURFACTANT; CATANIONIC VESICLES; LANGMUIR MONOLAYER; CONTRAST VARIATION; LIPOSOMES; MEMBRANES

KW - Bilayer; Monolayer; Piroxicam; SANS; AFM; MTT assay

KW - Monolayer

KW - Piroxicam

KW - SANS

KW - AFM

KW - Bilayer

KW - MTT assay

KW - BILAYER THICKNESS

KW - MEMBRANES

KW - X-RAY-SCATTERING

KW - PHOSPHATIDYLCHOLINE VESICLES

KW - CATIONIC SURFACTANT

KW - LANGMUIR MONOLAYER

KW - CONTRAST VARIATION

KW - CATANIONIC VESICLES

KW - LIPOSOMES

KW - ANGLE NEUTRON-SCATTERING

UR - http://www.scopus.com/inward/record.url?scp=85044160340&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/exploring-dual-impact-hydrocarbon-chainlength-role-piroxicam-conventional-nsaid-soylecithinion-pair

U2 - 10.1016/j.colsurfa.2018.03.025

DO - 10.1016/j.colsurfa.2018.03.025

M3 - Article

VL - 546

SP - 334

EP - 345

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

SN - 0927-7757

M1 - GB8CM

ER -