Some polar analytes (X) can reversibly form hydrates in water-containing eluents under the conditions of reversed-phase HPLC analysis, X + H 2O ⇄ X × H 2O. One of the methods to detect their formation is the recurrent approximation of the net retention times of such analytes, t R( C + Δ C) = at R( C) + b, where Δ C = const is the constant step in the variation of the organic modifier content of an eluent. These dependencies are linear if hydrates are not formed, but in the case of hydrate formation, they deviate from linearity under high water content. It has been shown that UV spectroscopic parameters, namely, relative optical densities: A rel = A(λ 1)/ A(λ 2), depend on eluent composition for some organic compounds, but their variations cannot be used as indicators for hydrate formation. The coefficients that characterize the dependence of the analyte retention indices on the organic component concentration of an eluent, dRI/ dC, appeared to be the most informative additional criterion for hydration. The values of these coefficients for most polar analytes are largely negative ( dRI/ dC < 0), whereas, for nonpolar compounds, they are largely positive ( dRI/ dC > 0).