Research output: Contribution to journal › Review article › peer-review
Evaluation of the ligand specificity of hemolymph hemoagglutinins and hemolysins of gastropod and bivalve molluscs. / Baskakov, A. V.; Polevshchikov, A. V.; Kharazova, A. D.
In: Journal of Evolutionary Biochemistry and Physiology, Vol. 36, No. 4, 01.01.2000, p. 367-374.Research output: Contribution to journal › Review article › peer-review
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TY - JOUR
T1 - Evaluation of the ligand specificity of hemolymph hemoagglutinins and hemolysins of gastropod and bivalve molluscs
AU - Baskakov, A. V.
AU - Polevshchikov, A. V.
AU - Kharazova, A. D.
PY - 2000/1/1
Y1 - 2000/1/1
N2 - The study deals with evaluation of ligand specificities of hemoagglutinins and hemolysins of hemolymph of three species of gastropods (Planorbius corneus, Lymnaea stagnalis, and Achatina fulica) and one species of bivalve molluscs (Anodonta cygnea). The hemoagglutinin titer was estimated from hemoagglutination reaction, the hemolysin titer, spectrophotometrically, from release of hemoglobin from sheep erythrocytes. The ligand specificity of hemoagglutinins and hemolysins was evaluated by an inhibitory modification of the hemoagglutination reaction. It has been shown that hemoagglutinins and hemolysins of pulmonary gastropod molluscs have different spectra of ligand specificity to the substances of the carbohydrate nature. Hemoagglutinins and hemolysins of the bivalve mollusc Anodonta cygnea have the same spectrum of ligand specificity. Among the ligands of these lectins, terminal N-acetylated sugars and monosaccharides predominate. Components of bacterial cell walls, lipopolysaccharide and phosphorylcholine, have been revealed among specific ligands of hemolymph hemoagglutinins and hemolysins.
AB - The study deals with evaluation of ligand specificities of hemoagglutinins and hemolysins of hemolymph of three species of gastropods (Planorbius corneus, Lymnaea stagnalis, and Achatina fulica) and one species of bivalve molluscs (Anodonta cygnea). The hemoagglutinin titer was estimated from hemoagglutination reaction, the hemolysin titer, spectrophotometrically, from release of hemoglobin from sheep erythrocytes. The ligand specificity of hemoagglutinins and hemolysins was evaluated by an inhibitory modification of the hemoagglutination reaction. It has been shown that hemoagglutinins and hemolysins of pulmonary gastropod molluscs have different spectra of ligand specificity to the substances of the carbohydrate nature. Hemoagglutinins and hemolysins of the bivalve mollusc Anodonta cygnea have the same spectrum of ligand specificity. Among the ligands of these lectins, terminal N-acetylated sugars and monosaccharides predominate. Components of bacterial cell walls, lipopolysaccharide and phosphorylcholine, have been revealed among specific ligands of hemolymph hemoagglutinins and hemolysins.
UR - http://www.scopus.com/inward/record.url?scp=27544492895&partnerID=8YFLogxK
U2 - 10.1007/BF02736986
DO - 10.1007/BF02736986
M3 - Review article
AN - SCOPUS:27544492895
VL - 36
SP - 367
EP - 374
JO - Journal of Evolutionary Biochemistry and Physiology
JF - Journal of Evolutionary Biochemistry and Physiology
SN - 0022-0930
IS - 4
ER -
ID: 41027644