For the first time in Russia, using groundbased measurements of direct solar infrared radiation, we derived data on ozone content in different layers of the atmosphere. The measurements were conducted with the help of a Bruker IFS125HR Fourier spectrometer in 2009–2012 in Petergof, which is 30 km west of the center of St. Petersburg. The errors in determining the ozone content by this method in the troposphere (0–12 km), in the stratosphere (12–50 km), in the layers of 10–20 and 20–50 km, and in the layers of 12–18, 18–25, and 25–50 km were ~4, 3, 3–5, and 4–7% (taking into account the instrumental and methodological errors, as well as the errors in specifying the temperature profile), respectively. The seasonal variation of tropospheric ozone content in the layer of 12–18 km is characterized by a clearly expressed maximum in March and a minimum in November, with amplitudes of 30 and 40%, respectively. For the layer of 18–25 km, the maximum and minimum are reached in the winter–spring period and late summer,