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Establishment of primary cell lines from tissues of the red-eared slider. / Kulak, Maria; Galkina, Svetlana; Saifitdinova, Alsu; Gaginskaya, Elena.

In: Biological Communications, Vol. 64, No. 4, 12.2019, p. 229-234.

Research output: Contribution to journal › Article › peer-review

Harvard

Kulak, M, Galkina, S, Saifitdinova, A & Gaginskaya, E 2019, 'Establishment of primary cell lines from tissues of the red-eared slider', Biological Communications, vol. 64, no. 4, pp. 229-234. https://doi.org/10.21638/spbu03.2019.401

APA

Kulak, M., Galkina, S., Saifitdinova, A., & Gaginskaya, E. (2019). Establishment of primary cell lines from tissues of the red-eared slider. Biological Communications, 64(4), 229-234. https://doi.org/10.21638/spbu03.2019.401

Vancouver

Kulak M, Galkina S, Saifitdinova A, Gaginskaya E. Establishment of primary cell lines from tissues of the red-eared slider. Biological Communications. 2019 Dec;64(4):229-234. https://doi.org/10.21638/spbu03.2019.401

Author

Kulak, Maria ; Galkina, Svetlana ; Saifitdinova, Alsu ; Gaginskaya, Elena. / Establishment of primary cell lines from tissues of the red-eared slider. In: Biological Communications. 2019 ; Vol. 64, No. 4. pp. 229-234.

BibTeX

@article{1493ab0144564c19876d3b4ef9ac0e6a,
title = "Establishment of primary cell lines from tissues of the red-eared slider",
abstract = "Primary cell lines of the red-eared slider were established from somatic slider tissues cultivated in appropriate medium for a certain period. Exponential cell growth started after 2 weeks of continuous cultivating. The cells were of various morphology resembling keratinocytes, fibroblasts, and melanocytes, some of them gathered to form dense conglomerates similar to so-called {"}embryoid bodies{"}. The cell lines were maintained for 3-7 passages before being taken for cytogenetic manipulations. Red-eared slider cell lines derived in this way can be cryopreserved and successfully recovered to provide a source of fixed cells for 3D imaging as well as a source of chromosomes and chromatin fiber preparations for cytological and cytogenetic studies. The protocol for red-eared slider cells was developed on the basis of the experiments described.",
keywords = "Explant, Primary culture, Trachemys scripta elegans, Turtles",
author = "Maria Kulak and Svetlana Galkina and Alsu Saifitdinova and Elena Gaginskaya",
year = "2019",
month = dec,
doi = "10.21638/spbu03.2019.401",
language = "English",
volume = "64",
pages = "229--234",
journal = "Biological Communications",
issn = "2542-2154",
publisher = "Издательство Санкт-Петербургского университета",
number = "4",

}

RIS

TY - JOUR

T1 - Establishment of primary cell lines from tissues of the red-eared slider

AU - Kulak, Maria

AU - Galkina, Svetlana

AU - Saifitdinova, Alsu

AU - Gaginskaya, Elena

PY - 2019/12

Y1 - 2019/12

N2 - Primary cell lines of the red-eared slider were established from somatic slider tissues cultivated in appropriate medium for a certain period. Exponential cell growth started after 2 weeks of continuous cultivating. The cells were of various morphology resembling keratinocytes, fibroblasts, and melanocytes, some of them gathered to form dense conglomerates similar to so-called "embryoid bodies". The cell lines were maintained for 3-7 passages before being taken for cytogenetic manipulations. Red-eared slider cell lines derived in this way can be cryopreserved and successfully recovered to provide a source of fixed cells for 3D imaging as well as a source of chromosomes and chromatin fiber preparations for cytological and cytogenetic studies. The protocol for red-eared slider cells was developed on the basis of the experiments described.

AB - Primary cell lines of the red-eared slider were established from somatic slider tissues cultivated in appropriate medium for a certain period. Exponential cell growth started after 2 weeks of continuous cultivating. The cells were of various morphology resembling keratinocytes, fibroblasts, and melanocytes, some of them gathered to form dense conglomerates similar to so-called "embryoid bodies". The cell lines were maintained for 3-7 passages before being taken for cytogenetic manipulations. Red-eared slider cell lines derived in this way can be cryopreserved and successfully recovered to provide a source of fixed cells for 3D imaging as well as a source of chromosomes and chromatin fiber preparations for cytological and cytogenetic studies. The protocol for red-eared slider cells was developed on the basis of the experiments described.

KW - Explant

KW - Primary culture

KW - Trachemys scripta elegans

KW - Turtles

UR - http://www.scopus.com/inward/record.url?scp=85085074007&partnerID=8YFLogxK

U2 - 10.21638/spbu03.2019.401

DO - 10.21638/spbu03.2019.401

M3 - Article

AN - SCOPUS:85085074007

VL - 64

SP - 229

EP - 234

JO - Biological Communications

JF - Biological Communications

SN - 2542-2154

IS - 4

ER -

ID: 60649193