Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development. / Koh, Tong Wey; Korolchuk, Viktor I.; Wairkar, Yogesh P.; Jiao, Wei; Evergren, Emma; Pan, Hongling; Zhou, Yi; Venken, Koen J.T.; Shupliakov, Oleg; Robinson, Iain M.; O'Kane, Cahir J.; Bellen, Hugo J.
In: Journal of Cell Biology, Vol. 178, No. 2, 16.07.2007, p. 309-322.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development
AU - Koh, Tong Wey
AU - Korolchuk, Viktor I.
AU - Wairkar, Yogesh P.
AU - Jiao, Wei
AU - Evergren, Emma
AU - Pan, Hongling
AU - Zhou, Yi
AU - Venken, Koen J.T.
AU - Shupliakov, Oleg
AU - Robinson, Iain M.
AU - O'Kane, Cahir J.
AU - Bellen, Hugo J.
PY - 2007/7/16
Y1 - 2007/7/16
N2 - Epidermal growth factor receptor pathway substrate clone 15 (Eps15) is a protein implicated in endocytosis, endosomal protein sorting, and cytoskeletal organization. Its role is, however, still unclear, because of reasons including limitations of dominant-negative experiments and apparent redundancy with other endocytic proteins. We generated Drosophila eps15-null mutants and show that Eps15 is required for proper synaptic bouton development and normal levels of synaptic vesicle (SV) endocytosis. Consistent with a role in SV endocytosis, Eps15 moves from the center of synaptic boutons to the periphery in response to synaptic activity. The endocytic protein, Dap160/intersectin, is a major binding partner of Eps15, and eps15 mutants phenotypically resemble dap160 mutants. Analyses of eps15 dap160 double mutants suggest that Eps15 functions in concert with Dap160 during SV endocytosis. Based on these data, we hypothesize that Eps15 and Dap160 promote the efficiency of endocytosis from the plasma membrane by maintaining high concentrations of multiple endocytic proteins, including dynamin, at synapses.
AB - Epidermal growth factor receptor pathway substrate clone 15 (Eps15) is a protein implicated in endocytosis, endosomal protein sorting, and cytoskeletal organization. Its role is, however, still unclear, because of reasons including limitations of dominant-negative experiments and apparent redundancy with other endocytic proteins. We generated Drosophila eps15-null mutants and show that Eps15 is required for proper synaptic bouton development and normal levels of synaptic vesicle (SV) endocytosis. Consistent with a role in SV endocytosis, Eps15 moves from the center of synaptic boutons to the periphery in response to synaptic activity. The endocytic protein, Dap160/intersectin, is a major binding partner of Eps15, and eps15 mutants phenotypically resemble dap160 mutants. Analyses of eps15 dap160 double mutants suggest that Eps15 functions in concert with Dap160 during SV endocytosis. Based on these data, we hypothesize that Eps15 and Dap160 promote the efficiency of endocytosis from the plasma membrane by maintaining high concentrations of multiple endocytic proteins, including dynamin, at synapses.
UR - http://www.scopus.com/inward/record.url?scp=34447524096&partnerID=8YFLogxK
U2 - 10.1083/jcb.200701030
DO - 10.1083/jcb.200701030
M3 - Article
C2 - 17620409
AN - SCOPUS:34447524096
VL - 178
SP - 309
EP - 322
JO - Journal of Cell Biology
JF - Journal of Cell Biology
SN - 0021-9525
IS - 2
ER -
ID: 40831966