Epidermal Growth Factor Causes the Decrease of CD146 Level Not Related to its Internalization in Human Endometrial Mesenchymal Stromal Cells

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Epidermal Growth Factor Causes the Decrease of CD146 Level Not Related to its Internalization in Human Endometrial Mesenchymal Stromal Cells. / Kamentseva, R. S.; Istomina, M. V.; Kharchenko, M. V.; Kornilova, E. S.

In: Cell and Tissue Biology, Vol. 15, No. 1, 01.2021, p. 19-23.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{9f514cfa1ae74e5a8ee547f1174133e5,

title = "Epidermal Growth Factor Causes the Decrease of CD146 Level Not Related to its Internalization in Human Endometrial Mesenchymal Stromal Cells",

abstract = "Abstract: We have previously shown using flow cytometry that during prolonged cultivation of endometrial MSC in the presence of EGF, the amount of CD146 on the plasma membrane decreased. One of the possible explanations is the increased non-specific CD146 internalization under such conditions. In this study, using confocal microscopy we showed that in the control cells CD146 was localized not only on the plasma membrane, but also in vesicles where it partially colocalized with markers of early and recycling endosomes. However, stimulation of endocytosis of the EGF receptor does not affect the number of CD146+ vesicles until at least 60 min, although the number of endosomes containing the EGF receptor increases significantly. In addition, only small portion of EGFR-positive endosomes contained CD146. Moreover, during cultivation in the presence of EGF for 5 days the total fluorescence intensity of both CD146 and the EGF receptor in the whole cell decreases. Thus, the decrease in the amount of CD146 during prolonged incubation in the presence of EGF is not associated with an increase of its endocytosis.",

keywords = "CD146, epidermal growth factor, human mesenchymal stromal cells",

author = "Kamentseva, {R. S.} and Istomina, {M. V.} and Kharchenko, {M. V.} and Kornilova, {E. S.}",

year = "2021",

month = jan,

doi = "10.1134/s1990519x21010053",

language = "English",

volume = "15",

pages = "19--23",

journal = "Cell and Tissue Biology",

issn = "1990-519X",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "1",

}

RIS

TY - JOUR

T1 - Epidermal Growth Factor Causes the Decrease of CD146 Level Not Related to its Internalization in Human Endometrial Mesenchymal Stromal Cells

AU - Kamentseva, R. S.

AU - Istomina, M. V.

AU - Kharchenko, M. V.

AU - Kornilova, E. S.

PY - 2021/1

Y1 - 2021/1

N2 - Abstract: We have previously shown using flow cytometry that during prolonged cultivation of endometrial MSC in the presence of EGF, the amount of CD146 on the plasma membrane decreased. One of the possible explanations is the increased non-specific CD146 internalization under such conditions. In this study, using confocal microscopy we showed that in the control cells CD146 was localized not only on the plasma membrane, but also in vesicles where it partially colocalized with markers of early and recycling endosomes. However, stimulation of endocytosis of the EGF receptor does not affect the number of CD146+ vesicles until at least 60 min, although the number of endosomes containing the EGF receptor increases significantly. In addition, only small portion of EGFR-positive endosomes contained CD146. Moreover, during cultivation in the presence of EGF for 5 days the total fluorescence intensity of both CD146 and the EGF receptor in the whole cell decreases. Thus, the decrease in the amount of CD146 during prolonged incubation in the presence of EGF is not associated with an increase of its endocytosis.

AB - Abstract: We have previously shown using flow cytometry that during prolonged cultivation of endometrial MSC in the presence of EGF, the amount of CD146 on the plasma membrane decreased. One of the possible explanations is the increased non-specific CD146 internalization under such conditions. In this study, using confocal microscopy we showed that in the control cells CD146 was localized not only on the plasma membrane, but also in vesicles where it partially colocalized with markers of early and recycling endosomes. However, stimulation of endocytosis of the EGF receptor does not affect the number of CD146+ vesicles until at least 60 min, although the number of endosomes containing the EGF receptor increases significantly. In addition, only small portion of EGFR-positive endosomes contained CD146. Moreover, during cultivation in the presence of EGF for 5 days the total fluorescence intensity of both CD146 and the EGF receptor in the whole cell decreases. Thus, the decrease in the amount of CD146 during prolonged incubation in the presence of EGF is not associated with an increase of its endocytosis.

KW - CD146

KW - epidermal growth factor

KW - human mesenchymal stromal cells

UR - http://www.scopus.com/inward/record.url?scp=85101260110&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/84a0fc8a-a274-3f60-bd91-5d57a4395c42/

U2 - 10.1134/s1990519x21010053

DO - 10.1134/s1990519x21010053

M3 - Article

AN - SCOPUS:85101260110

VL - 15

SP - 19

EP - 23

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 1

ER -

ID: 76656046