Epidermal growth factor causes the decrease of CD 146 level not related to its internalization in human endometrial mesenchymal stromal cells

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Epidermal growth factor causes the decrease of CD 146 level not related to its internalization in human endometrial mesenchymal stromal cells. / Kamentseva, R. S.; Istomina, M. V.; Kharchenko, M. V.; Kornilova, E. S.

In: Tsitologiya, Vol. 62, No. 7, 2020, p. 487-492.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{86b864a8ffd14b84bd2536b6d43a533d,

title = "Epidermal growth factor causes the decrease of CD 146 level not related to its internalization in human endometrial mesenchymal stromal cells",

abstract = "We have previously shown using flow cytometry that during prolonged cultivation of endometrial MSC in the presence of EGF, the amount of CD 146 on the plasma membrane decreased. One of the possible explanations is the increased non-specific CD 146 internalization under such conditions. In this study using confocal microscopy we showed that in the control cells CD 146 is localized not only on the plasma membrane, but also in vesicles where it partially colocalized with markers of early and recycling endosomes. However, stimulation of endocytosis of the EGF receptor does not affect the number of CD146+ vesicles until at least 60 min, although the number of endosomes containing the EGF receptor increases significantly. In addition, only small portion of EGFR-positive endosomes contained CD 146. Moreover, during cultivation in the presence of EGF for 5 days the total fluorescence intensity of both CD 146 and the EGF receptor in the whole cell decreases. Thus, the decrease in the amount of CD 146 during prolonged incubation in the presence of EGF is not associated with an increase of its endocytosis.",

keywords = "CD 146, Epidermal growth factor, Human mesenchymal stromal cells",

author = "Kamentseva, {R. S.} and Istomina, {M. V.} and Kharchenko, {M. V.} and Kornilova, {E. S.}",

year = "2020",

doi = "10.31857/S0041377120070020",

language = "English",

volume = "62",

pages = "487--492",

journal = "Цитология",

issn = "0041-3771",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "7",

}

RIS

TY - JOUR

T1 - Epidermal growth factor causes the decrease of CD 146 level not related to its internalization in human endometrial mesenchymal stromal cells

AU - Kamentseva, R. S.

AU - Istomina, M. V.

AU - Kharchenko, M. V.

AU - Kornilova, E. S.

PY - 2020

Y1 - 2020

N2 - We have previously shown using flow cytometry that during prolonged cultivation of endometrial MSC in the presence of EGF, the amount of CD 146 on the plasma membrane decreased. One of the possible explanations is the increased non-specific CD 146 internalization under such conditions. In this study using confocal microscopy we showed that in the control cells CD 146 is localized not only on the plasma membrane, but also in vesicles where it partially colocalized with markers of early and recycling endosomes. However, stimulation of endocytosis of the EGF receptor does not affect the number of CD146+ vesicles until at least 60 min, although the number of endosomes containing the EGF receptor increases significantly. In addition, only small portion of EGFR-positive endosomes contained CD 146. Moreover, during cultivation in the presence of EGF for 5 days the total fluorescence intensity of both CD 146 and the EGF receptor in the whole cell decreases. Thus, the decrease in the amount of CD 146 during prolonged incubation in the presence of EGF is not associated with an increase of its endocytosis.

AB - We have previously shown using flow cytometry that during prolonged cultivation of endometrial MSC in the presence of EGF, the amount of CD 146 on the plasma membrane decreased. One of the possible explanations is the increased non-specific CD 146 internalization under such conditions. In this study using confocal microscopy we showed that in the control cells CD 146 is localized not only on the plasma membrane, but also in vesicles where it partially colocalized with markers of early and recycling endosomes. However, stimulation of endocytosis of the EGF receptor does not affect the number of CD146+ vesicles until at least 60 min, although the number of endosomes containing the EGF receptor increases significantly. In addition, only small portion of EGFR-positive endosomes contained CD 146. Moreover, during cultivation in the presence of EGF for 5 days the total fluorescence intensity of both CD 146 and the EGF receptor in the whole cell decreases. Thus, the decrease in the amount of CD 146 during prolonged incubation in the presence of EGF is not associated with an increase of its endocytosis.

KW - CD 146

KW - Epidermal growth factor

KW - Human mesenchymal stromal cells

UR - http://www.scopus.com/inward/record.url?scp=85096317820&partnerID=8YFLogxK

U2 - 10.31857/S0041377120070020

DO - 10.31857/S0041377120070020

M3 - Article

AN - SCOPUS:85096317820

VL - 62

SP - 487

EP - 492

JO - Цитология

JF - Цитология

SN - 0041-3771

IS - 7

ER -

ID: 76656765