Research output: Contribution to journal › Article › peer-review
Endophyte Bacillus vallismortis BL01 to Control Fungal and Bacterial Phytopathogens of Tomato (Solanum lycopersicum L.) Plants. / Чеботарь, В.К.; Ганчева, Мария Семеновна; Чижевская, Е. П.; Ерофеева, А.В.; Хютти, А.В.; Лазарев, А.М.; Zhang, Xiuhai; Xue, Jing; Yang, Chunhong; Тихонович, Игорь Анатольевич.
In: Horticulturae, Vol. 10, No. 10, 1095, 14.10.2024.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Endophyte Bacillus vallismortis BL01 to Control Fungal and Bacterial Phytopathogens of Tomato (Solanum lycopersicum L.) Plants
AU - Чеботарь, В.К.
AU - Ганчева, Мария Семеновна
AU - Чижевская, Е. П.
AU - Ерофеева, А.В.
AU - Хютти, А.В.
AU - Лазарев, А.М.
AU - Zhang, Xiuhai
AU - Xue, Jing
AU - Yang, Chunhong
AU - Тихонович, Игорь Анатольевич
PY - 2024/10/14
Y1 - 2024/10/14
N2 - Some strains of Bacillus vallismortis have been reported to be efficient biocontrol agents against tomato pathogens. The aim of our study was to assess the biocontrol ability of the endophytic strain BL01 Bacillus vallismortis through in vitro and field trials, as well as to verify its plant colonization ability and analyze the bacterial genome in order to find genes responsible for the biocontrol activity. We demonstrated in a gnotobiotic system and by confocal laser microscopy that the endophytic strain BL01 was able to colonize the endosphere and rhizosphere of tomato, winter wheat and oilseed rape. In vitro experiments demonstrated the inhibition activity of BL01 against a wide range of phytopathogenic fungi and bacteria. BL01 showed biological efficacy in two-year field experiments with tomato plants against black bacterial spotting by 40–70.8% and against late blight by 47.1% and increased tomato harvest by 24.9% or 10.9 tons per hectare compared to the control. Genome analysis revealed the presence of genes that are responsible for the synthesis of biologically active secondary metabolites, which could be responsible for the biocontrol action. Strain BL01 B. vallismortis can be considered an effective biocontrol agent to control both fungal and bacterial diseases in tomato plants.
AB - Some strains of Bacillus vallismortis have been reported to be efficient biocontrol agents against tomato pathogens. The aim of our study was to assess the biocontrol ability of the endophytic strain BL01 Bacillus vallismortis through in vitro and field trials, as well as to verify its plant colonization ability and analyze the bacterial genome in order to find genes responsible for the biocontrol activity. We demonstrated in a gnotobiotic system and by confocal laser microscopy that the endophytic strain BL01 was able to colonize the endosphere and rhizosphere of tomato, winter wheat and oilseed rape. In vitro experiments demonstrated the inhibition activity of BL01 against a wide range of phytopathogenic fungi and bacteria. BL01 showed biological efficacy in two-year field experiments with tomato plants against black bacterial spotting by 40–70.8% and against late blight by 47.1% and increased tomato harvest by 24.9% or 10.9 tons per hectare compared to the control. Genome analysis revealed the presence of genes that are responsible for the synthesis of biologically active secondary metabolites, which could be responsible for the biocontrol action. Strain BL01 B. vallismortis can be considered an effective biocontrol agent to control both fungal and bacterial diseases in tomato plants.
KW - Bacillus vallismortis
KW - bacterial leaf spot
KW - colonization ability
KW - field tests
KW - genome analysis
KW - in vitro biocontrol activity
KW - late blight
UR - https://www.mendeley.com/catalogue/15be6f07-6338-3c02-bc12-3b0237012b7d/
U2 - 10.3390/horticulturae10101095
DO - 10.3390/horticulturae10101095
M3 - Article
VL - 10
JO - Horticulturae
JF - Horticulturae
SN - 2311-7524
IS - 10
M1 - 1095
ER -
ID: 126216076