Abstract: Versions of the electrophoretic determination of neutral carbohydrates by a method of indirect detection using acridone acetic and folic acids as absorbing additives (AA) are proposed. The effects of the nature and concentration of AA, alkali, and various modifiers (cetyltrimethylammonium bromide, CTAB) and ionic liquids (1-dodecyl-3-methylimidazolium chloride and 1-hexadecyl-3-methylimidazolium chloride) on the electrophoretic parameters of the migration of analytes are studied. The lowest limits of detection for carbohydrates are achieved in a background electrolyte containing 2.5 mM acridone acetic acid, 75 mM KOH, 0.5 mM CTAB, and 5 vol % MeOH, and amount to 4−10 μg/mL with an efficiency of up to 350 thousand t.p. Under the conditions found, samples of buckwheat honey and human blood plasma are analyzed. It is found that in analyzing food products, it is preferable to use folic acid as a AA, because it provides the best selectivity for the separation of carbohydrates: for the sucralose−sucrose pair, the resolution factor is 7.6. © Pleiades Publishing, Ltd. 2024. ISSN 1061-9348, Journal of Analytical Chemistry, 2024, Vol. 79, No. 2, pp. 224–232. Pleiades Publishing, Ltd., 2024.
Original languageEnglish
Pages (from-to)224-232
Number of pages9
JournalJournal of Analytical Chemistry
Issue number2
StatePublished - 1 Feb 2024

    Research areas

  • absorbing additives, capillary electrophoresis, carbohydrates, indirect detection, Additives, Ascorbic acid, Blood, Carbohydrates, Chlorine compounds, Electrolytes, Food products, Ionic liquids, Potassium hydroxide, Absorbing additive, Acridones, Additive alkali, Analytes, Cetyltrimethylammonium bromide, Detection methods, Folic acids, Indirect detection, Lower limits of detections, Methylimidazolium chloride, Capillary electrophoresis

ID: 117486828