Effects of ZnSO4-induced peripheral anosmia on zebrafish behavior and physiology

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Effects of ZnSO₄-induced peripheral anosmia on zebrafish behavior and physiology. / Abreu, Murilo S.; Giacomini, Ana C.V.V.; Rodriguez, Rubens; Kalueff, Allan V.; Barcellos, Leonardo J.G.

In: Behavioural Brain Research, Vol. 320, 01.03.2017, p. 275-281.

Research output: Contribution to journal › Article › peer-review

Author

Abreu, Murilo S. ; Giacomini, Ana C.V.V. ; Rodriguez, Rubens ; Kalueff, Allan V. ; Barcellos, Leonardo J.G. / Effects of ZnSO₄-induced peripheral anosmia on zebrafish behavior and physiology. In: Behavioural Brain Research. 2017 ; Vol. 320. pp. 275-281.

BibTeX

@article{2b1b47e657a94550992597221a36d503,

title = "Effects of ZnSO4-induced peripheral anosmia on zebrafish behavior and physiology",

abstract = "Olfaction plays a key role in modulating behavioral and physiological responses of various animal species, including fishes. Olfactory deficits can be induced in fish experimentally, and utilized to examine the role of olfaction in their normal and pathological behaviors. Here, we examine whether experimental anosmia, evoked by ZnSO4 in adult zebrafish can be associated with behavioral and/or physiological responses. We show that experimental ZnSO4-induced anosmia caused acute, but not prolonged, anxiogenic-like effects on zebrafish behavior tested in the novel tank test. The procedure also elevated whole-body cortisol levels in zebrafish. Moreover, ZnSO4 treatment, but not sham, produced damage to olfactory epithelium, inducing overt basal cell vacuolization and intercellular edema. The loss of olfaction, assessed by the fish food preference behavior in the aquatic Y-maze, was present 1 h, but not 24 h, after the treatment. Collectively, this suggests that transient experimental anosmia by ZnSO4 modulates zebrafish behavior and olfaction, which can be used to evoke and assess their stress-related anxiety-like states.",

keywords = "Anxiety, Emotional behavior, Experimental anosmia protocol, Olfactory deprivation, Zebrafish",

author = "Abreu, {Murilo S.} and Giacomini, {Ana C.V.V.} and Rubens Rodriguez and Kalueff, {Allan V.} and Barcellos, {Leonardo J.G.}",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2017",

month = mar,

day = "1",

doi = "10.1016/j.bbr.2016.12.014",

language = "English",

volume = "320",

pages = "275--281",

journal = "Behavioural Brain Research",

issn = "0166-4328",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Effects of ZnSO4-induced peripheral anosmia on zebrafish behavior and physiology

AU - Abreu, Murilo S.

AU - Giacomini, Ana C.V.V.

AU - Rodriguez, Rubens

AU - Kalueff, Allan V.

AU - Barcellos, Leonardo J.G.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Olfaction plays a key role in modulating behavioral and physiological responses of various animal species, including fishes. Olfactory deficits can be induced in fish experimentally, and utilized to examine the role of olfaction in their normal and pathological behaviors. Here, we examine whether experimental anosmia, evoked by ZnSO4 in adult zebrafish can be associated with behavioral and/or physiological responses. We show that experimental ZnSO4-induced anosmia caused acute, but not prolonged, anxiogenic-like effects on zebrafish behavior tested in the novel tank test. The procedure also elevated whole-body cortisol levels in zebrafish. Moreover, ZnSO4 treatment, but not sham, produced damage to olfactory epithelium, inducing overt basal cell vacuolization and intercellular edema. The loss of olfaction, assessed by the fish food preference behavior in the aquatic Y-maze, was present 1 h, but not 24 h, after the treatment. Collectively, this suggests that transient experimental anosmia by ZnSO4 modulates zebrafish behavior and olfaction, which can be used to evoke and assess their stress-related anxiety-like states.

AB - Olfaction plays a key role in modulating behavioral and physiological responses of various animal species, including fishes. Olfactory deficits can be induced in fish experimentally, and utilized to examine the role of olfaction in their normal and pathological behaviors. Here, we examine whether experimental anosmia, evoked by ZnSO4 in adult zebrafish can be associated with behavioral and/or physiological responses. We show that experimental ZnSO4-induced anosmia caused acute, but not prolonged, anxiogenic-like effects on zebrafish behavior tested in the novel tank test. The procedure also elevated whole-body cortisol levels in zebrafish. Moreover, ZnSO4 treatment, but not sham, produced damage to olfactory epithelium, inducing overt basal cell vacuolization and intercellular edema. The loss of olfaction, assessed by the fish food preference behavior in the aquatic Y-maze, was present 1 h, but not 24 h, after the treatment. Collectively, this suggests that transient experimental anosmia by ZnSO4 modulates zebrafish behavior and olfaction, which can be used to evoke and assess their stress-related anxiety-like states.

KW - Anxiety

KW - Emotional behavior

KW - Experimental anosmia protocol

KW - Olfactory deprivation

KW - Zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85007035044&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2016.12.014

DO - 10.1016/j.bbr.2016.12.014

M3 - Article

C2 - 27993691

VL - 320

SP - 275

EP - 281

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

ER -

ID: 7636469