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Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils. / Grigorieva, Daria V.; Gorudko, Irina V.; Shamova, Ekaterina V.; Terekhova, Maria S.; Maliushkova, Elena V.; Semak, Igor V.; Cherenkevich, Sergey N.; Sokolov, Alexey V.; Timoshenko, Alexander V.

In: Archives of Biochemistry and Biophysics, Vol. 675, 108122, 30.10.2019.

Research output: Contribution to journal › Article › peer-review

Harvard

Grigorieva, DV, Gorudko, IV, Shamova, EV, Terekhova, MS, Maliushkova, EV, Semak, IV, Cherenkevich, SN, Sokolov, AV & Timoshenko, AV 2019, 'Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils', Archives of Biochemistry and Biophysics, vol. 675, 108122. https://doi.org/10.1016/j.abb.2019.108122

APA

Grigorieva, D. V., Gorudko, I. V., Shamova, E. V., Terekhova, M. S., Maliushkova, E. V., Semak, I. V., Cherenkevich, S. N., Sokolov, A. V., & Timoshenko, A. V. (2019). Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils. Archives of Biochemistry and Biophysics, 675, [108122]. https://doi.org/10.1016/j.abb.2019.108122

Vancouver

Grigorieva DV, Gorudko IV, Shamova EV, Terekhova MS, Maliushkova EV, Semak IV et al. Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils. Archives of Biochemistry and Biophysics. 2019 Oct 30;675. 108122. https://doi.org/10.1016/j.abb.2019.108122

Author

Grigorieva, Daria V. ; Gorudko, Irina V. ; Shamova, Ekaterina V. ; Terekhova, Maria S. ; Maliushkova, Elena V. ; Semak, Igor V. ; Cherenkevich, Sergey N. ; Sokolov, Alexey V. ; Timoshenko, Alexander V. / Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils. In: Archives of Biochemistry and Biophysics. 2019 ; Vol. 675.

BibTeX

@article{9e38a3b0453d435b8368759cb9bf7ada,
title = "Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils",
abstract = "Lactoferrin is a non-heme iron-binding glycoprotein with multiple health-beneficial functions including antimicrobial, antioxidant, anticarcinogenic, and immunomodulatory effects. There is emerging evidence that neutrophils may serve as targets of lactoferrin in vivo, and here we show how recombinant human lactoferrin (rhLf) can contribute to this regulation. Indeed, our results demonstrate that rhLf binds efficiently to human neutrophils and induces a variety of early cellular responses such as mobilization of intracellular Ca2+, remodeling of actin cytoskeleton, and degranulation (release of lysozyme and myeloperoxidase). In addition, rhLf facilitates lectin-induced H2O2 production and stabilization of lectin-induced cellular aggregates. The role of calcium signaling seems to be essential for rhLf-induced activation of neutrophils, as Ca2+-chelators inhibit degranulation response while lectin-induced H2O2 production correlates significantly with cytoplasmic Ca2+ elevation. Taken together, our findings justify that rhLf can activate neutrophil functions in a calcium-dependent manner and hence, can potentiate innate immune responses.",
keywords = "Calcium ions, Cell aggregation, Degranulation, Neutrophils, Recombinant human lactoferrin, Respiratory burst",
author = "Grigorieva, {Daria V.} and Gorudko, {Irina V.} and Shamova, {Ekaterina V.} and Terekhova, {Maria S.} and Maliushkova, {Elena V.} and Semak, {Igor V.} and Cherenkevich, {Sergey N.} and Sokolov, {Alexey V.} and Timoshenko, {Alexander V.}",
note = "Funding Information: This work was partly supported by RFBR (grant 18-515-00004 ), BRFBR (grant B18R-058 ) and Russian President's grant MD-5133.2018.4 . Publisher Copyright: {\textcopyright} 2019 Elsevier Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2019",
month = oct,
day = "30",
doi = "10.1016/j.abb.2019.108122",
language = "English",
volume = "675",
journal = "Archives of Biochemistry and Biophysics",
issn = "0003-9861",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Effects of recombinant human lactoferrin on calcium signaling and functional responses of human neutrophils

AU - Grigorieva, Daria V.

AU - Gorudko, Irina V.

AU - Shamova, Ekaterina V.

AU - Terekhova, Maria S.

AU - Maliushkova, Elena V.

AU - Semak, Igor V.

AU - Cherenkevich, Sergey N.

AU - Sokolov, Alexey V.

AU - Timoshenko, Alexander V.

N1 - Funding Information: This work was partly supported by RFBR (grant 18-515-00004 ), BRFBR (grant B18R-058 ) and Russian President's grant MD-5133.2018.4 . Publisher Copyright: © 2019 Elsevier Inc. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2019/10/30

Y1 - 2019/10/30

N2 - Lactoferrin is a non-heme iron-binding glycoprotein with multiple health-beneficial functions including antimicrobial, antioxidant, anticarcinogenic, and immunomodulatory effects. There is emerging evidence that neutrophils may serve as targets of lactoferrin in vivo, and here we show how recombinant human lactoferrin (rhLf) can contribute to this regulation. Indeed, our results demonstrate that rhLf binds efficiently to human neutrophils and induces a variety of early cellular responses such as mobilization of intracellular Ca2+, remodeling of actin cytoskeleton, and degranulation (release of lysozyme and myeloperoxidase). In addition, rhLf facilitates lectin-induced H2O2 production and stabilization of lectin-induced cellular aggregates. The role of calcium signaling seems to be essential for rhLf-induced activation of neutrophils, as Ca2+-chelators inhibit degranulation response while lectin-induced H2O2 production correlates significantly with cytoplasmic Ca2+ elevation. Taken together, our findings justify that rhLf can activate neutrophil functions in a calcium-dependent manner and hence, can potentiate innate immune responses.

AB - Lactoferrin is a non-heme iron-binding glycoprotein with multiple health-beneficial functions including antimicrobial, antioxidant, anticarcinogenic, and immunomodulatory effects. There is emerging evidence that neutrophils may serve as targets of lactoferrin in vivo, and here we show how recombinant human lactoferrin (rhLf) can contribute to this regulation. Indeed, our results demonstrate that rhLf binds efficiently to human neutrophils and induces a variety of early cellular responses such as mobilization of intracellular Ca2+, remodeling of actin cytoskeleton, and degranulation (release of lysozyme and myeloperoxidase). In addition, rhLf facilitates lectin-induced H2O2 production and stabilization of lectin-induced cellular aggregates. The role of calcium signaling seems to be essential for rhLf-induced activation of neutrophils, as Ca2+-chelators inhibit degranulation response while lectin-induced H2O2 production correlates significantly with cytoplasmic Ca2+ elevation. Taken together, our findings justify that rhLf can activate neutrophil functions in a calcium-dependent manner and hence, can potentiate innate immune responses.

KW - Calcium ions

KW - Cell aggregation

KW - Degranulation

KW - Neutrophils

KW - Recombinant human lactoferrin

KW - Respiratory burst

UR - http://www.scopus.com/inward/record.url?scp=85072775669&partnerID=8YFLogxK

U2 - 10.1016/j.abb.2019.108122

DO - 10.1016/j.abb.2019.108122

M3 - Article

C2 - 31580874

AN - SCOPUS:85072775669

VL - 675

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

M1 - 108122

ER -

ID: 75024723