Research output: Contribution to journal › Article › peer-review
Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells. / Markova, Kseniia; Mikhailova, Valentina; Milyutina, Yulia; Korenevsky, Andrey; Sirotskaya, Anastasia; Rodygina, Veronika; Tyshchuk, Elizaveta; Grebenkina, Polina; Simbirtsev, Andrey; Selkov, Sergey; Sokolov, Dmitry.
In: International Journal of Molecular Sciences, Vol. 22, No. 24, 13663, 2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells
AU - Markova, Kseniia
AU - Mikhailova, Valentina
AU - Milyutina, Yulia
AU - Korenevsky, Andrey
AU - Sirotskaya, Anastasia
AU - Rodygina, Veronika
AU - Tyshchuk, Elizaveta
AU - Grebenkina, Polina
AU - Simbirtsev, Andrey
AU - Selkov, Sergey
AU - Sokolov, Dmitry
PY - 2021
Y1 - 2021
N2 - Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.
AB - Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.
KW - Caspase 3/metabolism
KW - Cell Communication
KW - Cell Line
KW - Cell Movement
KW - Cell Proliferation
KW - Cell-Derived Microparticles/metabolism
KW - Coculture Techniques
KW - Endothelial Cells/cytology
KW - Flow Cytometry
KW - Humans
KW - Interleukin-1beta/pharmacology
KW - Killer Cells, Natural/cytology
KW - Leukocyte Common Antigens/metabolism
KW - Phenotype
U2 - 10.3390/ijms222413663
DO - 10.3390/ijms222413663
M3 - Article
C2 - 34948459
VL - 22
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 24
M1 - 13663
ER -
ID: 91241852