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Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells. / Markova, Kseniia; Mikhailova, Valentina; Milyutina, Yulia; Korenevsky, Andrey; Sirotskaya, Anastasia; Rodygina, Veronika; Tyshchuk, Elizaveta; Grebenkina, Polina; Simbirtsev, Andrey; Selkov, Sergey; Sokolov, Dmitry.

In: International Journal of Molecular Sciences, Vol. 22, No. 24, 13663, 2021.

Research output: Contribution to journalArticlepeer-review

Harvard

Markova, K, Mikhailova, V, Milyutina, Y, Korenevsky, A, Sirotskaya, A, Rodygina, V, Tyshchuk, E, Grebenkina, P, Simbirtsev, A, Selkov, S & Sokolov, D 2021, 'Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells', International Journal of Molecular Sciences, vol. 22, no. 24, 13663. https://doi.org/10.3390/ijms222413663

APA

Markova, K., Mikhailova, V., Milyutina, Y., Korenevsky, A., Sirotskaya, A., Rodygina, V., Tyshchuk, E., Grebenkina, P., Simbirtsev, A., Selkov, S., & Sokolov, D. (2021). Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells. International Journal of Molecular Sciences, 22(24), [13663]. https://doi.org/10.3390/ijms222413663

Vancouver

Markova K, Mikhailova V, Milyutina Y, Korenevsky A, Sirotskaya A, Rodygina V et al. Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells. International Journal of Molecular Sciences. 2021;22(24). 13663. https://doi.org/10.3390/ijms222413663

Author

Markova, Kseniia ; Mikhailova, Valentina ; Milyutina, Yulia ; Korenevsky, Andrey ; Sirotskaya, Anastasia ; Rodygina, Veronika ; Tyshchuk, Elizaveta ; Grebenkina, Polina ; Simbirtsev, Andrey ; Selkov, Sergey ; Sokolov, Dmitry. / Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells. In: International Journal of Molecular Sciences. 2021 ; Vol. 22, No. 24.

BibTeX

@article{281af32641834abd946d257aa1f0b847,
title = "Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells",
abstract = "Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.",
keywords = "Caspase 3/metabolism, Cell Communication, Cell Line, Cell Movement, Cell Proliferation, Cell-Derived Microparticles/metabolism, Coculture Techniques, Endothelial Cells/cytology, Flow Cytometry, Humans, Interleukin-1beta/pharmacology, Killer Cells, Natural/cytology, Leukocyte Common Antigens/metabolism, Phenotype",
author = "Kseniia Markova and Valentina Mikhailova and Yulia Milyutina and Andrey Korenevsky and Anastasia Sirotskaya and Veronika Rodygina and Elizaveta Tyshchuk and Polina Grebenkina and Andrey Simbirtsev and Sergey Selkov and Dmitry Sokolov",
year = "2021",
doi = "10.3390/ijms222413663",
language = "English",
volume = "22",
journal = "International Journal of Molecular Sciences",
issn = "1422-0067",
publisher = "MDPI AG",
number = "24",

}

RIS

TY - JOUR

T1 - Effects of Microvesicles Derived from NK Cells Stimulated with IL-1β on the Phenotype and Functional Activity of Endothelial Cells

AU - Markova, Kseniia

AU - Mikhailova, Valentina

AU - Milyutina, Yulia

AU - Korenevsky, Andrey

AU - Sirotskaya, Anastasia

AU - Rodygina, Veronika

AU - Tyshchuk, Elizaveta

AU - Grebenkina, Polina

AU - Simbirtsev, Andrey

AU - Selkov, Sergey

AU - Sokolov, Dmitry

PY - 2021

Y1 - 2021

N2 - Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.

AB - Microvesicles (MVs) are plasma extracellular vesicles ranging from 100 (150) to 1000 nm in diameter. These are generally produced by different cells through their vital activity and are a source of various protein and non-protein molecules. It is assumed that MVs can mediate intercellular communication and modulate cell functions. The interaction between natural killer cells (NK cells) and endothelial cells underlies multiple pathological conditions. The ability of MVs derived from NK cells to influence the functional state of endothelial cells in inflammatory conditions has yet to be studied well. In this regard, we aimed to study the effects of MVs derived from NK cells of the NK-92 cell line stimulated with IL-1β on the phenotype, caspase activity, proliferation and migration of endothelial cells of the EA.hy926 cell line. Endothelial cells were cultured with MVs derived from cells of the NK-92 cell line after their stimulation with IL-1β. Using flow cytometry, we evaluated changes in the expression of endothelial cell surface molecules and endothelial cell death. We evaluated the effect of MVs derived from stimulated NK cells on the proliferative and migratory activity of endothelial cells, as well as the activation of caspase-3 and caspase-9 therein. It was established that the incubation of endothelial cells with MVs derived from cells of the NK-92 cell line stimulated with IL-1β and with MVs derived from unstimulated NK cells, leads to the decrease in the proliferative activity of endothelial cells, appearance of the pan leukocyte marker CD45 on them, caspase-3 activation and partial endothelial cell death, and reduced CD105 expression. However, compared with MVs derived from unstimulated NK cells, a more pronounced effect of MVs derived from cells of the NK-92 cell line stimulated with IL-1β was found in relation to the decrease in the endothelial cell migratory activity and the intensity of the CD54 molecule expression on them. The functional activity of MVs is therefore mediated by the conditions they are produced under, as well as their internal contents.

KW - Caspase 3/metabolism

KW - Cell Communication

KW - Cell Line

KW - Cell Movement

KW - Cell Proliferation

KW - Cell-Derived Microparticles/metabolism

KW - Coculture Techniques

KW - Endothelial Cells/cytology

KW - Flow Cytometry

KW - Humans

KW - Interleukin-1beta/pharmacology

KW - Killer Cells, Natural/cytology

KW - Leukocyte Common Antigens/metabolism

KW - Phenotype

U2 - 10.3390/ijms222413663

DO - 10.3390/ijms222413663

M3 - Article

C2 - 34948459

VL - 22

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 24

M1 - 13663

ER -

ID: 91241852