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Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation. / Lapina, Tatiana V.; Kochemasova, Lidiya Yu.; Forchhammer, Karl; Ermilova, Elena V.

In: Planta, Vol. 250, No. 4, 01.10.2019, p. 1379-1385.

Research output: Contribution to journal › Article › peer-review

Harvard

Lapina, TV, Kochemasova, LY, Forchhammer, K & Ermilova, EV 2019, 'Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation', Planta, vol. 250, no. 4, pp. 1379-1385. https://doi.org/10.1007/s00425-019-03249-5

APA

Lapina, T. V., Kochemasova, L. Y., Forchhammer, K., & Ermilova, E. V. (2019). Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation. Planta, 250(4), 1379-1385. https://doi.org/10.1007/s00425-019-03249-5

Vancouver

Lapina TV, Kochemasova LY, Forchhammer K, Ermilova EV. Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation. Planta. 2019 Oct 1;250(4):1379-1385. https://doi.org/10.1007/s00425-019-03249-5

Author

Lapina, Tatiana V. ; Kochemasova, Lidiya Yu. ; Forchhammer, Karl ; Ermilova, Elena V. / Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation. In: Planta. 2019 ; Vol. 250, No. 4. pp. 1379-1385.

BibTeX

@article{41bf426bed1b45d1adaa92b3807271d4,
title = "Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation",
abstract = "Main conclusion l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.",
keywords = "GLB-encoded signaling protein, Nitrogen, Nonphotosynthetic green algae, Oil droplets, SIGNAL-TRANSDUCTION PROTEIN, KINASE, ACETYL-COA CARBOXYLASE, ASSIMILATION, EXPRESSION",
author = "Lapina, {Tatiana V.} and Kochemasova, {Lidiya Yu.} and Karl Forchhammer and Ermilova, {Elena V.}",
note = "Lapina, T.V., Kochemasova, L.Y., Forchhammer, K. et al. Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation. Planta 250, 1379–1385 (2019). https://doi.org/10.1007/s00425-019-03249-5",
year = "2019",
month = oct,
day = "1",
doi = "10.1007/s00425-019-03249-5",
language = "English",
volume = "250",
pages = "1379--1385",
journal = "Planta",
issn = "0032-0935",
publisher = "Springer Nature",
number = "4",

}

RIS

TY - JOUR

T1 - Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation

AU - Lapina, Tatiana V.

AU - Kochemasova, Lidiya Yu.

AU - Forchhammer, Karl

AU - Ermilova, Elena V.

N1 - Lapina, T.V., Kochemasova, L.Y., Forchhammer, K. et al. Effects of arginine on Polytomella parva growth, PII protein levels and lipid body formation. Planta 250, 1379–1385 (2019). https://doi.org/10.1007/s00425-019-03249-5

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Main conclusion l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

AB - Main conclusion l-Arginine supports growth and resulted in increased PII signaling protein levels and lipid droplet accumulation in the colorless green alga Polytomella parva. Polytomella parva, a model system for nonphotosynthetic green algae, utilizes ammonium and several carbon sources, including ethanol and acetate. We previously reported that P. parva accumulates high amounts of arginine with the key enzyme of the ornithine/arginine biosynthesis pathway, N-acetyl-L-glutamate kinase, exhibiting high activity. Here we demonstrate that l-arginine can be used by this alga as a nitrogen source. Externally supplied arginine directly influenced the levels of PII signaling protein and formation of triacylglycerol (TAG)-filled lipid bodies (LBs). Our results suggest that the nitrogen source, but not nitrogen starvation, may be critical for the accumulation of LBs in a PII-independent manner in P. parva.

KW - GLB-encoded signaling protein

KW - Nitrogen

KW - Nonphotosynthetic green algae

KW - Oil droplets

KW - SIGNAL-TRANSDUCTION PROTEIN

KW - KINASE

KW - ACETYL-COA CARBOXYLASE

KW - ASSIMILATION

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=85069939056&partnerID=8YFLogxK

U2 - 10.1007/s00425-019-03249-5

DO - 10.1007/s00425-019-03249-5

M3 - Article

AN - SCOPUS:85069939056

VL - 250

SP - 1379

EP - 1385

JO - Planta

JF - Planta

SN - 0032-0935

IS - 4

ER -

ID: 45860669