Dynamics of PpIX accumulation in A549, HeLa and 3T3 cell lines

DOI

https://doi.org/10.1109/ICLO48556.2020.9285888
Final published version

D. A. Gorbenko
A. V. Belashov
T. N. Belyaeva
E. S. Kornilova
I. V. Semenova
O. S. Vasyutinskii

The synthesis and accumulation of protoporphyrin IX (PpIX), induced by 5-aminolevulinic acid (5-ALA) in HeLa, A549 and 3T3 cells was studied using confocal fluorescence microscopy. The accumulation dynamics of PpIX in cells was determined from the kinetics of its fluorescence intensity. The PpIX synthesis rate was analyzed as function of 5-ALA concentration in the culture medium and incubation time.

Original language	English
Title of host publication	Proceedings - International Conference Laser Optics 2020, ICLO 2020
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781728152332
DOIs	https://doi.org/10.1109/ICLO48556.2020.9285888
State	Published - 2 Nov 2020
Event	2020 International Conference Laser Optics, ICLO 2020 - St. Petersburg, Russian Federation Duration: 2 Nov 2020 → 6 Nov 2020

Publication series

Name	Proceedings - International Conference Laser Optics 2020, ICLO 2020

Conference

Conference	2020 International Conference Laser Optics, ICLO 2020
Country/Territory	Russian Federation
City	St. Petersburg
Period	2/11/20 → 6/11/20

Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics

Research areas

5-aminolevulinic acid, confocal fluorescence microscopy, photodynamic therapy, protoporphyrin IX

ID: 76656469