Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure

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Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure. / Livanova, Alexandra A. ; Fedorova, Arina A. ; Zavirsky, Alexander V. ; Bikmurzina, Anastasia E. ; Krivoi, Igor I. ; Markov, Alexander G. .

In: Physiological Reports, Vol. 9, No. 15, e14960, 08.2021, p. e14960.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{8b92573e20fe4bddac41948daec3e37c,

title = "Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure",

abstract = "Ionizing radiation causes dramatic change in the transport and barrier functions of the intestine. The degree of radiation damage rate depends primarily on the absorbed dose and post-irradiation time. Variety of experimental protocols providing different time points and doses exist, with the lack of a common approach. In this study, to develop a unified convenient experimental scheme, dose and time dependence of barrier and transport properties of rat jejunum following ionizing radiation exposure were examined. Male Wistar rats were exposed to total body X-ray irradiation (2, 5, or 10 Gy). The control group was subjected to sham irradiation procedure. Samples of rat jejunum were obtained at 24, 48, or 72 h post-irradiation. Transepithelial resistance, short circuit current (Isc), and paracellular permeability for sodium fluorescein of jejunum samples were measured in an Ussing chamber; a histological examination was also performed. These parameters were significantly disturbed only 72 h after irradiation at a dose of 10 Gy, which was accompanied by loss of crypt and villi, inflammatory infiltrations, and disintegration of enterocytes. This suggests that found experimental point (72 h after 10 Gy exposure) is the most appropriate for future study using rat jejunum as a model.",

keywords = "ионизирующая радиация, тонкая кишка, камера уссинга, Ionizing radiation, jejunum, Ussing chamber, ionizing radiation",

author = "Livanova, {Alexandra A.} and Fedorova, {Arina A.} and Zavirsky, {Alexander V.} and Bikmurzina, {Anastasia E.} and Krivoi, {Igor I.} and Markov, {Alexander G.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society",

year = "2021",

month = aug,

doi = "10.14814/phy2.14960",

language = "English",

volume = "9",

pages = "e14960",

journal = "Physiological Reports",

issn = "2051-817X",

publisher = "Wiley-Blackwell",

number = "15",

}

RIS

TY - JOUR

T1 - Dose and time dependence of functional impairments in rat jejunum following ionizing radiation exposure

AU - Livanova, Alexandra A.

AU - Fedorova, Arina A.

AU - Zavirsky, Alexander V.

AU - Bikmurzina, Anastasia E.

AU - Krivoi, Igor I.

AU - Markov, Alexander G.

PY - 2021/8

Y1 - 2021/8

N2 - Ionizing radiation causes dramatic change in the transport and barrier functions of the intestine. The degree of radiation damage rate depends primarily on the absorbed dose and post-irradiation time. Variety of experimental protocols providing different time points and doses exist, with the lack of a common approach. In this study, to develop a unified convenient experimental scheme, dose and time dependence of barrier and transport properties of rat jejunum following ionizing radiation exposure were examined. Male Wistar rats were exposed to total body X-ray irradiation (2, 5, or 10 Gy). The control group was subjected to sham irradiation procedure. Samples of rat jejunum were obtained at 24, 48, or 72 h post-irradiation. Transepithelial resistance, short circuit current (Isc), and paracellular permeability for sodium fluorescein of jejunum samples were measured in an Ussing chamber; a histological examination was also performed. These parameters were significantly disturbed only 72 h after irradiation at a dose of 10 Gy, which was accompanied by loss of crypt and villi, inflammatory infiltrations, and disintegration of enterocytes. This suggests that found experimental point (72 h after 10 Gy exposure) is the most appropriate for future study using rat jejunum as a model.

AB - Ionizing radiation causes dramatic change in the transport and barrier functions of the intestine. The degree of radiation damage rate depends primarily on the absorbed dose and post-irradiation time. Variety of experimental protocols providing different time points and doses exist, with the lack of a common approach. In this study, to develop a unified convenient experimental scheme, dose and time dependence of barrier and transport properties of rat jejunum following ionizing radiation exposure were examined. Male Wistar rats were exposed to total body X-ray irradiation (2, 5, or 10 Gy). The control group was subjected to sham irradiation procedure. Samples of rat jejunum were obtained at 24, 48, or 72 h post-irradiation. Transepithelial resistance, short circuit current (Isc), and paracellular permeability for sodium fluorescein of jejunum samples were measured in an Ussing chamber; a histological examination was also performed. These parameters were significantly disturbed only 72 h after irradiation at a dose of 10 Gy, which was accompanied by loss of crypt and villi, inflammatory infiltrations, and disintegration of enterocytes. This suggests that found experimental point (72 h after 10 Gy exposure) is the most appropriate for future study using rat jejunum as a model.

KW - ионизирующая радиация

KW - тонкая кишка

KW - камера уссинга

KW - Ionizing radiation

KW - jejunum

KW - Ussing chamber

KW - ionizing radiation

UR - http://www.scopus.com/inward/record.url?scp=85112393128&partnerID=8YFLogxK

U2 - 10.14814/phy2.14960

DO - 10.14814/phy2.14960

M3 - Article

VL - 9

SP - e14960

JO - Physiological Reports

JF - Physiological Reports

SN - 2051-817X

IS - 15

M1 - e14960

ER -

ID: 84630735