Research output: Contribution to journal › Article › peer-review
DNA interaction with synthetic polymers in solution. / Kasyanenko, Nina; Afanasieva, Daria; Dribinsky, Boris; Mukhin, Dmitry; Nazarova, Olga; Panarin, Evgeny.
In: Structural Chemistry, Vol. 18, No. 4, 08.2007, p. 519-525.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - DNA interaction with synthetic polymers in solution
AU - Kasyanenko, Nina
AU - Afanasieva, Daria
AU - Dribinsky, Boris
AU - Mukhin, Dmitry
AU - Nazarova, Olga
AU - Panarin, Evgeny
PY - 2007/8
Y1 - 2007/8
N2 - DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,N- trimethylammonio)ethyl methacrylate chloride (PTMAEM), poly-l-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P ≈ 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made.
AB - DNA complexes with cationic polymers (polyvinylamine (PVA), polyallylamine (PAA), polydimethylaminoethylmethacrylate (PDMAEM), poly-(N,N,N- trimethylammonio)ethyl methacrylate chloride (PTMAEM), poly-l-lysine (PLL)) were investigated. It was shown that volume and persistent length of DNA do not change essentially at low cationic polymer concentration in a solution. DNA packaging in 0.005 M NaCl was observed at charge ratio N/P ≈ 1. Secondary DNA structure in complexes was not disrupted, and DNA was protected from protonation. The comparison between DNA packaging in complexes with polycations and DNA condensation induced by trivalent ions was made.
KW - DNA condensation
KW - DNA-polymeric complexes
KW - Poly-L-lysine
KW - Polyallylamine
KW - Polydimethylaminoethylmethacrylate
KW - Polytrimethylammonioethyl methacrylate
KW - Polyvinylamine
UR - http://www.scopus.com/inward/record.url?scp=34447501896&partnerID=8YFLogxK
U2 - 10.1007/s11224-007-9162-1
DO - 10.1007/s11224-007-9162-1
M3 - Article
AN - SCOPUS:34447501896
VL - 18
SP - 519
EP - 525
JO - Structural Chemistry
JF - Structural Chemistry
SN - 1040-0400
IS - 4
ER -
ID: 73390737