DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution

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DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. / Wang, Xumei; Gussarova, Galina; Ruhsam, Markus; de Vere, Natasha; Metherell, Chris; Hollingsworth, Peter M.; Twyford, Alex D.

In: AoB PLANTS, Vol. 10, No. 3, ply026, 01.05.2018.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{f4d91ab9739346889590bd9dbff63878,

title = "DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution",

abstract = "DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British Euphrasia (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British Euphrasia, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows Euphrasia have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British Euphrasia species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera.",

keywords = "British flora, DNA barcoding, Euphrasia, Orobanchaceae, Phylogeny, Polyploidy, Taxonomic complexity",

author = "Xumei Wang and Galina Gussarova and Markus Ruhsam and {de Vere}, Natasha and Chris Metherell and Hollingsworth, {Peter M.} and Twyford, {Alex D.}",

note = "Funding Information: This study formed part of an international exchange programme for X.W., funded by the Chinese Scholarship Council (CSC). P.M.H. and M.R. acknowledge funding from the Scottish Government{\textquoteright}s Rural and Environment Science and Analytical Services Division (RESAS). Research by G.G. is supported by the Research Council of Norway: Projects N248799 and N257642; and Norwegian Taxonomy Initiative: Project N70184215. Research by A.D.T. is supported by a Natural Environment Research Council (NERC) Fellowship NE/L011336/1. Publisher Copyright: {\textcopyright} The Author(s) 2018. Published by Oxford University Press on behalf of the Annals of Botany Company. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",

year = "2018",

month = may,

day = "1",

doi = "10.1093/aobpla/ply026",

language = "English",

volume = "10",

journal = "AoB PLANTS",

issn = "2041-2851",

publisher = "Oxford University Press",

number = "3",

}

RIS

TY - JOUR

T1 - DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution

AU - Wang, Xumei

AU - Gussarova, Galina

AU - Ruhsam, Markus

AU - de Vere, Natasha

AU - Metherell, Chris

AU - Hollingsworth, Peter M.

AU - Twyford, Alex D.

N1 - Funding Information: This study formed part of an international exchange programme for X.W., funded by the Chinese Scholarship Council (CSC). P.M.H. and M.R. acknowledge funding from the Scottish Government’s Rural and Environment Science and Analytical Services Division (RESAS). Research by G.G. is supported by the Research Council of Norway: Projects N248799 and N257642; and Norwegian Taxonomy Initiative: Project N70184215. Research by A.D.T. is supported by a Natural Environment Research Council (NERC) Fellowship NE/L011336/1. Publisher Copyright: © The Author(s) 2018. Published by Oxford University Press on behalf of the Annals of Botany Company. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2018/5/1

Y1 - 2018/5/1

N2 - DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British Euphrasia (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British Euphrasia, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows Euphrasia have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British Euphrasia species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera.

AB - DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British Euphrasia (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British Euphrasia, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows Euphrasia have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British Euphrasia species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera.

KW - British flora

KW - DNA barcoding

KW - Euphrasia

KW - Orobanchaceae

KW - Phylogeny

KW - Polyploidy

KW - Taxonomic complexity

UR - http://www.scopus.com/inward/record.url?scp=85047825917&partnerID=8YFLogxK

U2 - 10.1093/aobpla/ply026

DO - 10.1093/aobpla/ply026

M3 - Article

AN - SCOPUS:85047825917

VL - 10

JO - AoB PLANTS

JF - AoB PLANTS

SN - 2041-2851

IS - 3

M1 - ply026

ER -

ID: 71629085