Differential protein expression of mitogen-activated protein kinases in erythrocytes and liver of lamprey Lampetra fluviatilis on the course of prespawning starvation

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Differential protein expression of mitogen-activated protein kinases in erythrocytes and liver of lamprey Lampetra fluviatilis on the course of prespawning starvation. / Khvorova, Irina A.; Nadei, Olga V.; Agalakova, Natalia I.

In: Comparative Biochemistry and Physiology -Part A : Molecular and Integrative Physiology, Vol. 264, 111108, 02.2022.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{0b29e91943264fe59264a790a63a00b5,

title = "Differential protein expression of mitogen-activated protein kinases in erythrocytes and liver of lamprey Lampetra fluviatilis on the course of prespawning starvation",

abstract = "The study was designed to identify the types of mitogen-activated protein kinases (MAPKs) in erythrocytes and liver tissues of river lamprey Lampetra fluviatilis and monitor the changes in protein expression levels of found enzymes on the course of prespawning starvation (from November to the end of May). Immunoreactivity of the native and phosphorylated forms of ERK1/2, JNK and p38 was examined in the cytosolic and membrane cell fractions. Both lamprey erythrocytes and liver were found to highly express ERK1/2 and JNK, whereas only trace amounts of p38 were revealed in hepatic tissues. ERK1/2 was identified in cytosolic and membrane fractions, whereas JNK and p38 were predominantly cytosolic enzymes. Total cellular amounts of ERK1/2 and phospho-ERK1/2 in both erythrocytes and liver tissues appeared to be relatively stable on the course of prespawning starvation. However, before spawning ERK1/2 translocated from cytosol to membranes, with partial decline of its cytoplasmic expression being compensated by increases in membrane-bound pool. Immunoreactivity of cytoplasmic JNK, phospho-JNK and p38 were stable from November to March, but sharply decreased before spawning exhibiting almost negligible levels in May, which suggests the depletion of their cellular fractions. Most probably, ERK1/2 plays more important role in mediating adaptive responses of erythrocytes and liver tissues to conditions of natural starvation and maintenance of cell viability before spawning and death of animals in May.",

keywords = "Erythrocytes, Lamprey, Liver, Mitogen-activated protein kinases, Prespawning starvation, Subcellular Fractions/enzymology, Male, Liver/enzymology, Starvation/blood, Erythrocytes/enzymology, Reproduction, Animals, Mitogen-Activated Protein Kinases/blood, Female, Lampreys/blood, Seasons, Fish Proteins/blood",

author = "Khvorova, {Irina A.} and Nadei, {Olga V.} and Agalakova, {Natalia I.}",

note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",

year = "2022",

month = feb,

doi = "10.1016/j.cbpa.2021.111108",

language = "English",

volume = "264",

journal = "Comparative Biochemistry and Physiology. Part A: Physiology",

issn = "1095-6433",

publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Differential protein expression of mitogen-activated protein kinases in erythrocytes and liver of lamprey Lampetra fluviatilis on the course of prespawning starvation

AU - Khvorova, Irina A.

AU - Nadei, Olga V.

AU - Agalakova, Natalia I.

PY - 2022/2

Y1 - 2022/2

N2 - The study was designed to identify the types of mitogen-activated protein kinases (MAPKs) in erythrocytes and liver tissues of river lamprey Lampetra fluviatilis and monitor the changes in protein expression levels of found enzymes on the course of prespawning starvation (from November to the end of May). Immunoreactivity of the native and phosphorylated forms of ERK1/2, JNK and p38 was examined in the cytosolic and membrane cell fractions. Both lamprey erythrocytes and liver were found to highly express ERK1/2 and JNK, whereas only trace amounts of p38 were revealed in hepatic tissues. ERK1/2 was identified in cytosolic and membrane fractions, whereas JNK and p38 were predominantly cytosolic enzymes. Total cellular amounts of ERK1/2 and phospho-ERK1/2 in both erythrocytes and liver tissues appeared to be relatively stable on the course of prespawning starvation. However, before spawning ERK1/2 translocated from cytosol to membranes, with partial decline of its cytoplasmic expression being compensated by increases in membrane-bound pool. Immunoreactivity of cytoplasmic JNK, phospho-JNK and p38 were stable from November to March, but sharply decreased before spawning exhibiting almost negligible levels in May, which suggests the depletion of their cellular fractions. Most probably, ERK1/2 plays more important role in mediating adaptive responses of erythrocytes and liver tissues to conditions of natural starvation and maintenance of cell viability before spawning and death of animals in May.

AB - The study was designed to identify the types of mitogen-activated protein kinases (MAPKs) in erythrocytes and liver tissues of river lamprey Lampetra fluviatilis and monitor the changes in protein expression levels of found enzymes on the course of prespawning starvation (from November to the end of May). Immunoreactivity of the native and phosphorylated forms of ERK1/2, JNK and p38 was examined in the cytosolic and membrane cell fractions. Both lamprey erythrocytes and liver were found to highly express ERK1/2 and JNK, whereas only trace amounts of p38 were revealed in hepatic tissues. ERK1/2 was identified in cytosolic and membrane fractions, whereas JNK and p38 were predominantly cytosolic enzymes. Total cellular amounts of ERK1/2 and phospho-ERK1/2 in both erythrocytes and liver tissues appeared to be relatively stable on the course of prespawning starvation. However, before spawning ERK1/2 translocated from cytosol to membranes, with partial decline of its cytoplasmic expression being compensated by increases in membrane-bound pool. Immunoreactivity of cytoplasmic JNK, phospho-JNK and p38 were stable from November to March, but sharply decreased before spawning exhibiting almost negligible levels in May, which suggests the depletion of their cellular fractions. Most probably, ERK1/2 plays more important role in mediating adaptive responses of erythrocytes and liver tissues to conditions of natural starvation and maintenance of cell viability before spawning and death of animals in May.

KW - Erythrocytes

KW - Lamprey

KW - Liver

KW - Mitogen-activated protein kinases

KW - Prespawning starvation

KW - Subcellular Fractions/enzymology

KW - Male

KW - Liver/enzymology

KW - Starvation/blood

KW - Erythrocytes/enzymology

KW - Reproduction

KW - Animals

KW - Mitogen-Activated Protein Kinases/blood

KW - Female

KW - Lampreys/blood

KW - Seasons

KW - Fish Proteins/blood

UR - http://www.scopus.com/inward/record.url?scp=85118490773&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/c81f37a0-a3bb-3bee-897c-1961e5b4a3d1/

U2 - 10.1016/j.cbpa.2021.111108

DO - 10.1016/j.cbpa.2021.111108

M3 - Article

C2 - 34728403

AN - SCOPUS:85118490773

VL - 264

JO - Comparative Biochemistry and Physiology. Part A: Physiology

JF - Comparative Biochemistry and Physiology. Part A: Physiology

SN - 1095-6433

M1 - 111108

ER -

ID: 98434276