TY - JOUR

T1 - Differential expression of CD42b and CD9 proteins in platelets and extracellular membrane vesicles during platelet-concentrate storage

AU - Kishenko, V. V.

AU - Kondratov, K. A.

AU - Belyakova, M. V.

AU - Mikhailovskii, V. Yu

AU - Sidorkevich, S. V.

AU - Vavilova, T. V.

AU - Fedorov, A. V.

AU - Sirotkina, O. V.

N1 - Kishenko, V.V., Kondratov, K.A., Belyakova, M.V. et al. Differential expression of CD42b and CD9 proteins in platelets and extracellular membrane vesicles during platelet-concentrate storage. Cell Tiss. Biol. 11, 440–446 (2017). https://doi.org/10.1134/S1990519X17060050

PY - 2017

Y1 - 2017

N2 - Platelet concentrate is used to restore and maintain hemostasis in patients with a reduced number or activity of platelets. Platelet concentrate changes its properties when stored, which leads to a weakening of the therapeutic effect, as well as the occurrence of transfusion side effects. One of the processes that alter platelet concentrates during storage is the secretion of several types of membrane vesicles. Despite the fact that platelet-derived membrane vesicles affect homeostasis and transmit signals of intercellular communication, mechanisms of their formation, features of regulatory activity and molecular composition are still poorly understood. In this paper, the results of analysis of CD42b and CD9 membrane proteins expression in platelets and platelet-derived membrane vesicles during platelet-concentrate storage are presented. Populations of membrane vesicles of different sizes were isolated from the platelet concentrates and characterized. Aggregation and morphological alteration of platelets are observed during storage; the protein composition of platelets, as well as membrane vesicles, changes, and there is a significant increase in the levels of CD42b and CD9 proteins in fractions of membrane vesicles. The results obtained indicate that platelet concentrates contain different populations of membrane vesicles, the molecular composition of which varies during storage.

AB - Platelet concentrate is used to restore and maintain hemostasis in patients with a reduced number or activity of platelets. Platelet concentrate changes its properties when stored, which leads to a weakening of the therapeutic effect, as well as the occurrence of transfusion side effects. One of the processes that alter platelet concentrates during storage is the secretion of several types of membrane vesicles. Despite the fact that platelet-derived membrane vesicles affect homeostasis and transmit signals of intercellular communication, mechanisms of their formation, features of regulatory activity and molecular composition are still poorly understood. In this paper, the results of analysis of CD42b and CD9 membrane proteins expression in platelets and platelet-derived membrane vesicles during platelet-concentrate storage are presented. Populations of membrane vesicles of different sizes were isolated from the platelet concentrates and characterized. Aggregation and morphological alteration of platelets are observed during storage; the protein composition of platelets, as well as membrane vesicles, changes, and there is a significant increase in the levels of CD42b and CD9 proteins in fractions of membrane vesicles. The results obtained indicate that platelet concentrates contain different populations of membrane vesicles, the molecular composition of which varies during storage.

KW - CD42b

KW - CD9

KW - differential centrifugation

KW - exosomes

KW - extracellular membrane vesicles

KW - microvesicles

KW - platelet activation

KW - platelet-concentrate storage

UR - http://www.scopus.com/inward/record.url?scp=85038262837&partnerID=8YFLogxK

U2 - 10.1134/S1990519X17060050

DO - 10.1134/S1990519X17060050

M3 - Article

AN - SCOPUS:85038262837

VL - 11

SP - 440

EP - 446

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 6

ER -