Research output: Contribution to journal › Article
Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications. / Voitechovi, E.; Bratov, A.; Abramova, N.; Razumiene, J.; Kirsanov, D.; Legin, A. (Author and editor); Lakshmi, D.; Piletsky, S.; Whitcombe, M.; Ivanova-Mitseva, P.K.
In: Electrochimica Acta, Vol. 173, 2015, p. 59-66.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development of label-free impedimetric platform based on new conductive polyaniline polymer and three-dimensional interdigitated electrode array for biosensor applications
AU - Voitechovi, E.
AU - Bratov, A.
AU - Abramova, N.
AU - Razumiene, J.
AU - Kirsanov, D.
AU - Lakshmi, D.
AU - Piletsky, S.
AU - Whitcombe, M.
AU - Ivanova-Mitseva, P.K.
A2 - Legin, A.
PY - 2015
Y1 - 2015
N2 - © 2015 Elsevier Ltd. All rights reserved. Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N',N'-diethyldithiocarbamoylethylamidoethyl) aniline (AnD) was deposited onto 3D-IDEA by chemical polymerisation. It was found that the polymer film resistance depends on the redox-potential of the solution. For the first time polyAnD was used as enzyme immobilisation matrix. Pyrroloquinolinequinone (PQQ) dependent alcohol and glucose dehydrogenases were immobilized on 3D-IDEA covered with polyAnD by two different methods. 3D-IDEA sensors with enzymes, which were immobilised by physisorption on polyAnD layer, showed specific response in the presence of 1 μM of the corresponding substrates. Obtained results revealed that PQQ dependent dehydrogenases can re-oxidize on polyAnD vi
AB - © 2015 Elsevier Ltd. All rights reserved. Novel label-free impedimetric platform based on a three-dimensional interdigitated electrode array (3D-IDEA) sensor and new conductive polymer as a transducer for oxidoreductases is introduced. This platform is cost-effective, simple to construct and miniaturize. Monomer of conductive polymer N-(N',N'-diethyldithiocarbamoylethylamidoethyl) aniline (AnD) was deposited onto 3D-IDEA by chemical polymerisation. It was found that the polymer film resistance depends on the redox-potential of the solution. For the first time polyAnD was used as enzyme immobilisation matrix. Pyrroloquinolinequinone (PQQ) dependent alcohol and glucose dehydrogenases were immobilized on 3D-IDEA covered with polyAnD by two different methods. 3D-IDEA sensors with enzymes, which were immobilised by physisorption on polyAnD layer, showed specific response in the presence of 1 μM of the corresponding substrates. Obtained results revealed that PQQ dependent dehydrogenases can re-oxidize on polyAnD vi
U2 - 10.1016/j.electacta.2015.05.011
DO - 10.1016/j.electacta.2015.05.011
M3 - Article
VL - 173
SP - 59
EP - 66
JO - Electrochimica Acta
JF - Electrochimica Acta
SN - 0013-4686
ER -
ID: 3975414