Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
Determination of citric acid in urine by enzymatic flow injection system based on a novel microfluidic chip. / Sun, Qiyong; Tu, Jiawei; Yaroshenko, Irma; Kirsanov, Dmitry; Legin, Andrey; Wang, Ping.
11TH ASIAN CONFERENCE ON CHEMICAL SENSORS. ed. / M Noor; AYM Shakaff; AH Adom; N Miura; CW Lin; A Zakaria; A Tuantranont; SK ZaAba; R Ngadiran; WY Chung; A Harun; SM Mamduh; N VanHieu; LM Kamarudin; NAH Zahri; SMMS Zakaria. Elsevier, 2016. p. 52-55 (Procedia Chemistry; Vol. 20).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
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TY - GEN
T1 - Determination of citric acid in urine by enzymatic flow injection system based on a novel microfluidic chip
AU - Sun, Qiyong
AU - Tu, Jiawei
AU - Yaroshenko, Irma
AU - Kirsanov, Dmitry
AU - Legin, Andrey
AU - Wang, Ping
PY - 2016
Y1 - 2016
N2 - A flow injection analysis (FIA) system with enzymatic detection has been proposed for determination of citric acid in urine using a novel microfluidic chip. A microfluidic device based on PDMS channels is designed and fabricated. To determinate the citric acid concentration in real samples and explore interference factors, three models are established based on different standard solutions of citrate, which are prepared with deionized water, artificial urine and real sample without citrate, respectively. The correlation coefficients of these models are 0.9989, 0.9955 and 0.9976 within the range from 0.1 mM to 6 mM. Meanwhile, a strong correlation is obtained between the three models (Each R-2>0.986) with regression analysis. At last, 20 urine samples are tested and an analysis of the correlation between the results measured by the proposed method and by the capillary electrophoresis method is made. Results indicate that this method looks promising in urine citrate analysis because of its high sensitivity, high selectivity and low consumption of solvent. (C) 2016 Published by Elsevier B.V.
AB - A flow injection analysis (FIA) system with enzymatic detection has been proposed for determination of citric acid in urine using a novel microfluidic chip. A microfluidic device based on PDMS channels is designed and fabricated. To determinate the citric acid concentration in real samples and explore interference factors, three models are established based on different standard solutions of citrate, which are prepared with deionized water, artificial urine and real sample without citrate, respectively. The correlation coefficients of these models are 0.9989, 0.9955 and 0.9976 within the range from 0.1 mM to 6 mM. Meanwhile, a strong correlation is obtained between the three models (Each R-2>0.986) with regression analysis. At last, 20 urine samples are tested and an analysis of the correlation between the results measured by the proposed method and by the capillary electrophoresis method is made. Results indicate that this method looks promising in urine citrate analysis because of its high sensitivity, high selectivity and low consumption of solvent. (C) 2016 Published by Elsevier B.V.
KW - Flow injection analysis
KW - Enzymatic detection
KW - Citric acid
KW - Microfluidic device
U2 - 10.1016/j.proche.2016.07.008
DO - 10.1016/j.proche.2016.07.008
M3 - статья в сборнике материалов конференции
T3 - Procedia Chemistry
SP - 52
EP - 55
BT - 11TH ASIAN CONFERENCE ON CHEMICAL SENSORS
A2 - Noor, M
A2 - Shakaff, AYM
A2 - Adom, AH
A2 - Miura, N
A2 - Lin, CW
A2 - Zakaria, A
A2 - Tuantranont, A
A2 - ZaAba, SK
A2 - Ngadiran, R
A2 - Chung, WY
A2 - Harun, A
A2 - Mamduh, SM
A2 - VanHieu, N
A2 - Kamarudin, LM
A2 - Zahri, NAH
A2 - Zakaria, SMMS
PB - Elsevier
Y2 - 16 November 2015 through 17 November 2015
ER -
ID: 125643626