TY - JOUR

T1 - Detection of various autoantibodies in patients with pulmonary sarcoidosis

AU - Zinchenko, Ulia

AU - Starshinova, Anna

AU - Basantsova, Natalia

AU - Malkova, Anna

AU - Surkova, Elena

AU - Lapin, Sergey

AU - Gildburd, Boris

AU - Churilov, Leonid

AU - Yablonskiy, Piotr

PY - 2019/9/28

Y1 - 2019/9/28

N2 - Introduction: Sarcoidosis (SD) – is a granulomatous disease with unknown nature. The autoimmune nature of the disease is considered as one of the possible theories. An increase level of several autoantibodies was published in patients with SD: antinuclear antibodies, rheumatoid factor, antibodies to cyclic citrullinated peptides, antibodies to double-stranded DNA (Kobak, 2014; Weinberg, 2000).Material and methods: A prospective study with the inclusion of 56 patients with verified pulmonary SD was conducted in 2018. 20 different autoantibodies were studied using indirect immunofluorescence, immunoblotting, enzyme immunoassay. Statistical analysis was performed using GraphPad 5.0. The study was supported by the grant from the Russian Federation Government (contract № 14.W03.31.0009 of 13.02.2017).Results: The most significant results were obtained in the following antibodies: to Hep-2-cell antigens (ANA IIF) - 7% (5/75); to beta2-glycoprotein (b2GPI) - 8% (6/75); to thyroperoxidase (ATP) - 8% (6/75); to tyroglobulin (aTG) - 9% (7/75); rheumatoid factor (RF) -15% (11/75); component C3 complement systems (C3) – 40% (30/75); complement system component C4 (C4) - 25% (19/75); to mutated citrullinated vimentin (MCV) - 39% (22/57).Conclusion: The total frequency of the detection of different autoantibodies in SD varies widely. The frequency of antibodies to the MCV detected in 39%, that allows us to consider this laboratory indicator as one of the main targets of the immunological reaction in SD.

AB - Introduction: Sarcoidosis (SD) – is a granulomatous disease with unknown nature. The autoimmune nature of the disease is considered as one of the possible theories. An increase level of several autoantibodies was published in patients with SD: antinuclear antibodies, rheumatoid factor, antibodies to cyclic citrullinated peptides, antibodies to double-stranded DNA (Kobak, 2014; Weinberg, 2000).Material and methods: A prospective study with the inclusion of 56 patients with verified pulmonary SD was conducted in 2018. 20 different autoantibodies were studied using indirect immunofluorescence, immunoblotting, enzyme immunoassay. Statistical analysis was performed using GraphPad 5.0. The study was supported by the grant from the Russian Federation Government (contract № 14.W03.31.0009 of 13.02.2017).Results: The most significant results were obtained in the following antibodies: to Hep-2-cell antigens (ANA IIF) - 7% (5/75); to beta2-glycoprotein (b2GPI) - 8% (6/75); to thyroperoxidase (ATP) - 8% (6/75); to tyroglobulin (aTG) - 9% (7/75); rheumatoid factor (RF) -15% (11/75); component C3 complement systems (C3) – 40% (30/75); complement system component C4 (C4) - 25% (19/75); to mutated citrullinated vimentin (MCV) - 39% (22/57).Conclusion: The total frequency of the detection of different autoantibodies in SD varies widely. The frequency of antibodies to the MCV detected in 39%, that allows us to consider this laboratory indicator as one of the main targets of the immunological reaction in SD.

KW - Immunology

KW - Adults

KW - Sarcoidosis

U2 - 10.1183/13993003.congress-2019.PA1380

DO - 10.1183/13993003.congress-2019.PA1380

M3 - тезисы

VL - 54

JO - European Respiratory Journal

JF - European Respiratory Journal

SN - 0903-1936

IS - S63

M1 - PA 1380

Y2 - 28 September 2019 through 2 October 2019

ER -