Detection of an atypical Paramecium bursaria chlorella symbiont

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Detection of an atypical Paramecium bursaria chlorella symbiont. / Vorobyev, K.P.; Andronov, E.E.; Rautian, M.; Skoblo, I.; Migunova, A.V.; Kvitko, K.V.

In: Protistology, Vol. 6, No. 1, 2009, p. 39-44.

Research output: Contribution to journal › Article

BibTeX

@article{caf840cf2f4a4a269c5599ac70e7c4d3,

title = "Detection of an atypical Paramecium bursaria chlorella symbiont",

abstract = "A useful approach to rapid identification of Chlorella strains in a single cell of Paramecium bursaria was developed. A “nested” PCR-based method with primers flanking intron insertion sites in the 18S rRNA gene of Chlorella ensured fast screening of large P. bursaria collections for the presence of the two basic types of Chlorella endosymbionts without isolating them in pure culture. The method applied to several Paramecium isolates showed that the P. bursaria clone T-24-5 from Tajikistan presumably bears an atypical Chlorella symbiont possessing an atypical intron in the 18S rRNA gene. This sequence was cloned and sequenced. BLAST analyses of the intron sequence cloned showed the presence of 136-bp stretch highly similar to the Chlorella virus intron fragment in major capsid protein Vp54 gene. The similarity value indicated a common origin of the sequences and suggested that the horizontal transfer of this DNA fragment had happened between the chlorella and the virus attacking it.",

keywords = "Paramecium bursaria, Chlorella, PBCV, 18S rRNA gene, group I intron",

author = "K.P. Vorobyev and E.E. Andronov and M. Rautian and I. Skoblo and A.V. Migunova and K.V. Kvitko",

year = "2009",

language = "English",

volume = "6",

pages = "39--44",

journal = "Protistology",

issn = "1680-0826",

publisher = "Protozoological Society Affiliated With The Russian Academy Of Sciences",

number = "1",

}

RIS

TY - JOUR

T1 - Detection of an atypical Paramecium bursaria chlorella symbiont

AU - Vorobyev, K.P.

AU - Andronov, E.E.

AU - Rautian, M.

AU - Skoblo, I.

AU - Migunova, A.V.

AU - Kvitko, K.V.

PY - 2009

Y1 - 2009

N2 - A useful approach to rapid identification of Chlorella strains in a single cell of Paramecium bursaria was developed. A “nested” PCR-based method with primers flanking intron insertion sites in the 18S rRNA gene of Chlorella ensured fast screening of large P. bursaria collections for the presence of the two basic types of Chlorella endosymbionts without isolating them in pure culture. The method applied to several Paramecium isolates showed that the P. bursaria clone T-24-5 from Tajikistan presumably bears an atypical Chlorella symbiont possessing an atypical intron in the 18S rRNA gene. This sequence was cloned and sequenced. BLAST analyses of the intron sequence cloned showed the presence of 136-bp stretch highly similar to the Chlorella virus intron fragment in major capsid protein Vp54 gene. The similarity value indicated a common origin of the sequences and suggested that the horizontal transfer of this DNA fragment had happened between the chlorella and the virus attacking it.

AB - A useful approach to rapid identification of Chlorella strains in a single cell of Paramecium bursaria was developed. A “nested” PCR-based method with primers flanking intron insertion sites in the 18S rRNA gene of Chlorella ensured fast screening of large P. bursaria collections for the presence of the two basic types of Chlorella endosymbionts without isolating them in pure culture. The method applied to several Paramecium isolates showed that the P. bursaria clone T-24-5 from Tajikistan presumably bears an atypical Chlorella symbiont possessing an atypical intron in the 18S rRNA gene. This sequence was cloned and sequenced. BLAST analyses of the intron sequence cloned showed the presence of 136-bp stretch highly similar to the Chlorella virus intron fragment in major capsid protein Vp54 gene. The similarity value indicated a common origin of the sequences and suggested that the horizontal transfer of this DNA fragment had happened between the chlorella and the virus attacking it.

KW - Paramecium bursaria

KW - Chlorella

KW - PBCV

KW - 18S rRNA gene

KW - group I intron

M3 - Article

VL - 6

SP - 39

EP - 44

JO - Protistology

JF - Protistology

SN - 1680-0826

IS - 1

ER -

ID: 5112753