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Decellularized mitral valve in a long-term sheep model. / Iablonskii, Pavel; Cebotari, Serghei; Ciubotaru, Anatol; Sarikouch, Samir; Hoeffler, Klaus; Hilfiker, Andres; Haverich, Axel; Tudorache, Igor.

In: European Journal of Cardio-thoracic Surgery, Vol. 53, No. 6, 01.06.2018, p. 1165-1172.

Research output: Contribution to journalArticlepeer-review

Harvard

Iablonskii, P, Cebotari, S, Ciubotaru, A, Sarikouch, S, Hoeffler, K, Hilfiker, A, Haverich, A & Tudorache, I 2018, 'Decellularized mitral valve in a long-term sheep model', European Journal of Cardio-thoracic Surgery, vol. 53, no. 6, pp. 1165-1172. https://doi.org/10.1093/ejcts/ezx485

APA

Iablonskii, P., Cebotari, S., Ciubotaru, A., Sarikouch, S., Hoeffler, K., Hilfiker, A., Haverich, A., & Tudorache, I. (2018). Decellularized mitral valve in a long-term sheep model. European Journal of Cardio-thoracic Surgery, 53(6), 1165-1172. https://doi.org/10.1093/ejcts/ezx485

Vancouver

Iablonskii P, Cebotari S, Ciubotaru A, Sarikouch S, Hoeffler K, Hilfiker A et al. Decellularized mitral valve in a long-term sheep model. European Journal of Cardio-thoracic Surgery. 2018 Jun 1;53(6):1165-1172. https://doi.org/10.1093/ejcts/ezx485

Author

Iablonskii, Pavel ; Cebotari, Serghei ; Ciubotaru, Anatol ; Sarikouch, Samir ; Hoeffler, Klaus ; Hilfiker, Andres ; Haverich, Axel ; Tudorache, Igor. / Decellularized mitral valve in a long-term sheep model. In: European Journal of Cardio-thoracic Surgery. 2018 ; Vol. 53, No. 6. pp. 1165-1172.

BibTeX

@article{106e9866a2474c22adbbdf92fb30100c,
title = "Decellularized mitral valve in a long-term sheep model",
abstract = "OBJECTIVES: The objective of this study was to evaluate surgical handling, in vivo hemodynamic performance and morphological characteristics of decellularized mitral valves (DMVs) in a long-term sheep model. METHODS: Ovine mitral valves were decellularized using detergents and β-mercaptoethanol. Orthotopic implantations were performed in 6-month-old sheep (41.3 ± 1.2 kg, n = 11) without annulus reinforcement. Commercially available stented porcine aortic valves [biological mitral valve (BMV), n = 3] were implanted conventionally and used as controls. Valve function was evaluated by transoesophageal echocardiography and explants were investigated by a routine bright field microscopy and immunofluorescent histology. RESULTS: During implantation, 2 DMVs required cleft closure of the anterior leaflet. All valves were competent on water test and early postoperative transoesophageal echocardiography. Six animals (DMV, n = 4; BMV, n = 2) survived 12 months. Six animals died within the first 4 months due to valve-related complications. At 12 months, transoesophageal echocardiography revealed severe degeneration in all BMVs. Macroscopically, BMV revealed calcification at the commissures and leaflet insertion area. Histological examination showed sporadic cells negative for endothelial nitric oxide synthase, von Willebrand factor and CD45 on their surface. In contrast, DMV showed no calcification or stenosis, and the regurgitation was trivial to moderate in all animals. Fibrotic hardening occurred only along the suture line of the valve annulus, immunostaining revealed collagen IV covering the entire leaflet surface and a repopulation with endothelial cells. CONCLUSIONS: Surgical implantation of DMV is feasible and results in good early graft function. Additional in vivo investigations are required to minimize the procedure-related complications and to increase the reproducibility of surgical implantation. Degenerative profile of allogeneic DMV is superior to commercially available porcine aortic prosthesis.",
keywords = "Decellularization, Mitral valve replacement, Tissue engineering",
author = "Pavel Iablonskii and Serghei Cebotari and Anatol Ciubotaru and Samir Sarikouch and Klaus Hoeffler and Andres Hilfiker and Axel Haverich and Igor Tudorache",
note = "Funding Information: This work was supported by CORTISS Hannover, Herz-und Gewebeforschungs GmbH. Publisher Copyright: {\textcopyright} The Author(s) 2017/2018. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.",
year = "2018",
month = jun,
day = "1",
doi = "10.1093/ejcts/ezx485",
language = "English",
volume = "53",
pages = "1165--1172",
journal = "European Journal of Cardio-thoracic Surgery",
issn = "1010-7940",
publisher = "Elsevier",
number = "6",

}

RIS

TY - JOUR

T1 - Decellularized mitral valve in a long-term sheep model

AU - Iablonskii, Pavel

AU - Cebotari, Serghei

AU - Ciubotaru, Anatol

AU - Sarikouch, Samir

AU - Hoeffler, Klaus

AU - Hilfiker, Andres

AU - Haverich, Axel

AU - Tudorache, Igor

N1 - Funding Information: This work was supported by CORTISS Hannover, Herz-und Gewebeforschungs GmbH. Publisher Copyright: © The Author(s) 2017/2018. Published by Oxford University Press on behalf of the European Association for Cardio-Thoracic Surgery. All rights reserved. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.

PY - 2018/6/1

Y1 - 2018/6/1

N2 - OBJECTIVES: The objective of this study was to evaluate surgical handling, in vivo hemodynamic performance and morphological characteristics of decellularized mitral valves (DMVs) in a long-term sheep model. METHODS: Ovine mitral valves were decellularized using detergents and β-mercaptoethanol. Orthotopic implantations were performed in 6-month-old sheep (41.3 ± 1.2 kg, n = 11) without annulus reinforcement. Commercially available stented porcine aortic valves [biological mitral valve (BMV), n = 3] were implanted conventionally and used as controls. Valve function was evaluated by transoesophageal echocardiography and explants were investigated by a routine bright field microscopy and immunofluorescent histology. RESULTS: During implantation, 2 DMVs required cleft closure of the anterior leaflet. All valves were competent on water test and early postoperative transoesophageal echocardiography. Six animals (DMV, n = 4; BMV, n = 2) survived 12 months. Six animals died within the first 4 months due to valve-related complications. At 12 months, transoesophageal echocardiography revealed severe degeneration in all BMVs. Macroscopically, BMV revealed calcification at the commissures and leaflet insertion area. Histological examination showed sporadic cells negative for endothelial nitric oxide synthase, von Willebrand factor and CD45 on their surface. In contrast, DMV showed no calcification or stenosis, and the regurgitation was trivial to moderate in all animals. Fibrotic hardening occurred only along the suture line of the valve annulus, immunostaining revealed collagen IV covering the entire leaflet surface and a repopulation with endothelial cells. CONCLUSIONS: Surgical implantation of DMV is feasible and results in good early graft function. Additional in vivo investigations are required to minimize the procedure-related complications and to increase the reproducibility of surgical implantation. Degenerative profile of allogeneic DMV is superior to commercially available porcine aortic prosthesis.

AB - OBJECTIVES: The objective of this study was to evaluate surgical handling, in vivo hemodynamic performance and morphological characteristics of decellularized mitral valves (DMVs) in a long-term sheep model. METHODS: Ovine mitral valves were decellularized using detergents and β-mercaptoethanol. Orthotopic implantations were performed in 6-month-old sheep (41.3 ± 1.2 kg, n = 11) without annulus reinforcement. Commercially available stented porcine aortic valves [biological mitral valve (BMV), n = 3] were implanted conventionally and used as controls. Valve function was evaluated by transoesophageal echocardiography and explants were investigated by a routine bright field microscopy and immunofluorescent histology. RESULTS: During implantation, 2 DMVs required cleft closure of the anterior leaflet. All valves were competent on water test and early postoperative transoesophageal echocardiography. Six animals (DMV, n = 4; BMV, n = 2) survived 12 months. Six animals died within the first 4 months due to valve-related complications. At 12 months, transoesophageal echocardiography revealed severe degeneration in all BMVs. Macroscopically, BMV revealed calcification at the commissures and leaflet insertion area. Histological examination showed sporadic cells negative for endothelial nitric oxide synthase, von Willebrand factor and CD45 on their surface. In contrast, DMV showed no calcification or stenosis, and the regurgitation was trivial to moderate in all animals. Fibrotic hardening occurred only along the suture line of the valve annulus, immunostaining revealed collagen IV covering the entire leaflet surface and a repopulation with endothelial cells. CONCLUSIONS: Surgical implantation of DMV is feasible and results in good early graft function. Additional in vivo investigations are required to minimize the procedure-related complications and to increase the reproducibility of surgical implantation. Degenerative profile of allogeneic DMV is superior to commercially available porcine aortic prosthesis.

KW - Decellularization

KW - Mitral valve replacement

KW - Tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=85048093720&partnerID=8YFLogxK

U2 - 10.1093/ejcts/ezx485

DO - 10.1093/ejcts/ezx485

M3 - Article

C2 - 29385428

AN - SCOPUS:85048093720

VL - 53

SP - 1165

EP - 1172

JO - European Journal of Cardio-thoracic Surgery

JF - European Journal of Cardio-thoracic Surgery

SN - 1010-7940

IS - 6

ER -

ID: 74299400