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Crystallization of the isobutylphosphocholine-cholesterol-isobutanol (1:3:3) complex and its investigation by X-ray analysis : Interaction of phopholipid headgroups with cholesterol. / Karasev, Vladimir A.; Fundamensky, Vladimir S.; Bannova, Irina I.; Franke, Valerija D.; Stefanov, Vassily E.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1466, No. 1-2, 01.06.2000, p. 23-38.

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Karasev, Vladimir A. ; Fundamensky, Vladimir S. ; Bannova, Irina I. ; Franke, Valerija D. ; Stefanov, Vassily E. / Crystallization of the isobutylphosphocholine-cholesterol-isobutanol (1:3:3) complex and its investigation by X-ray analysis : Interaction of phopholipid headgroups with cholesterol. In: Biochimica et Biophysica Acta - Biomembranes. 2000 ; Vol. 1466, No. 1-2. pp. 23-38.

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@article{3854503935e5420d935761e659cdfcaa,
title = "Crystallization of the isobutylphosphocholine-cholesterol-isobutanol (1:3:3) complex and its investigation by X-ray analysis: Interaction of phopholipid headgroups with cholesterol",
abstract = "A crystal complex consisting of the isobutyl analog of phosphatidylcholine (PC) (isobutylphosphocholine), cholesterol, and isobutanol with molecular ratio 1:3:3 was obtained and investigated by means of X-ray analysis. The complex was shown to correspond to the monoclinic system (sp. gr. P21): a=16.994(10), b=11.314(7), c=28.164(15), β=104.07(3), V=5252.63 A3, Z=2, D(calc)=1.0273 g/cm3. The isobutylphosphocholine molecule is the key component of the complex. Pairs of hydrogen bonds are formed between the (-δ)O-P-O(δ-) group of the isobutylphosphocholine molecule and C-OH groups of two cholesterol and two isobutanol molecules. The third molecules of cholesterol and isobutanol are H-bonded with the (-δ)O-P-O(δ-) group of the isobutylphosphocholine molecule via C-OH groups of isobutanol and cholesterol, respectively. The crystal structure is built up by translation of the complex in multiplicate along the two-fold axis in the direction of axis b. It contains bands formed by isobutylphosphocholine molecules alternately changing their direction. They are fixed by virtue of two zones of electrostatic interactions of the type (-δ)O-P-O(δ-)...+N(CH3)3 and are more or less parallel to the bc plane. The structure also contains three-layer domains formed by cholesterol molecules perpendicular to isobutylphosphocholine bands. In the direction of the c-axis isobutylphosphocholine bands alternate with the layers of cholesterol molecules herewith reproducing repeated blocks. The obtained structure is compared with that of crystals of phospholipids and cholesterol and its derivatives. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Biomembrane modeling, Cholesterol, Crystal complex, Phospholipid analog, X-ray analysis",
author = "Karasev, {Vladimir A.} and Fundamensky, {Vladimir S.} and Bannova, {Irina I.} and Franke, {Valerija D.} and Stefanov, {Vassily E.}",
note = "Funding Information: We are grateful to Dr. V.V. Luchinin for helpful discussion. A part of this work was supported by the research Grant from Russian Foundation for Basic Research (code 99-04-49836).",
year = "2000",
month = jun,
day = "1",
doi = "10.1016/S0005-2736(00)00162-0",
language = "English",
volume = "1466",
pages = "23--38",
journal = "Biochimica et Biophysica Acta - Biomembranes",
issn = "0005-2736",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Crystallization of the isobutylphosphocholine-cholesterol-isobutanol (1:3:3) complex and its investigation by X-ray analysis

T2 - Interaction of phopholipid headgroups with cholesterol

AU - Karasev, Vladimir A.

AU - Fundamensky, Vladimir S.

AU - Bannova, Irina I.

AU - Franke, Valerija D.

AU - Stefanov, Vassily E.

N1 - Funding Information: We are grateful to Dr. V.V. Luchinin for helpful discussion. A part of this work was supported by the research Grant from Russian Foundation for Basic Research (code 99-04-49836).

PY - 2000/6/1

Y1 - 2000/6/1

N2 - A crystal complex consisting of the isobutyl analog of phosphatidylcholine (PC) (isobutylphosphocholine), cholesterol, and isobutanol with molecular ratio 1:3:3 was obtained and investigated by means of X-ray analysis. The complex was shown to correspond to the monoclinic system (sp. gr. P21): a=16.994(10), b=11.314(7), c=28.164(15), β=104.07(3), V=5252.63 A3, Z=2, D(calc)=1.0273 g/cm3. The isobutylphosphocholine molecule is the key component of the complex. Pairs of hydrogen bonds are formed between the (-δ)O-P-O(δ-) group of the isobutylphosphocholine molecule and C-OH groups of two cholesterol and two isobutanol molecules. The third molecules of cholesterol and isobutanol are H-bonded with the (-δ)O-P-O(δ-) group of the isobutylphosphocholine molecule via C-OH groups of isobutanol and cholesterol, respectively. The crystal structure is built up by translation of the complex in multiplicate along the two-fold axis in the direction of axis b. It contains bands formed by isobutylphosphocholine molecules alternately changing their direction. They are fixed by virtue of two zones of electrostatic interactions of the type (-δ)O-P-O(δ-)...+N(CH3)3 and are more or less parallel to the bc plane. The structure also contains three-layer domains formed by cholesterol molecules perpendicular to isobutylphosphocholine bands. In the direction of the c-axis isobutylphosphocholine bands alternate with the layers of cholesterol molecules herewith reproducing repeated blocks. The obtained structure is compared with that of crystals of phospholipids and cholesterol and its derivatives. Copyright (C) 2000 Elsevier Science B.V.

AB - A crystal complex consisting of the isobutyl analog of phosphatidylcholine (PC) (isobutylphosphocholine), cholesterol, and isobutanol with molecular ratio 1:3:3 was obtained and investigated by means of X-ray analysis. The complex was shown to correspond to the monoclinic system (sp. gr. P21): a=16.994(10), b=11.314(7), c=28.164(15), β=104.07(3), V=5252.63 A3, Z=2, D(calc)=1.0273 g/cm3. The isobutylphosphocholine molecule is the key component of the complex. Pairs of hydrogen bonds are formed between the (-δ)O-P-O(δ-) group of the isobutylphosphocholine molecule and C-OH groups of two cholesterol and two isobutanol molecules. The third molecules of cholesterol and isobutanol are H-bonded with the (-δ)O-P-O(δ-) group of the isobutylphosphocholine molecule via C-OH groups of isobutanol and cholesterol, respectively. The crystal structure is built up by translation of the complex in multiplicate along the two-fold axis in the direction of axis b. It contains bands formed by isobutylphosphocholine molecules alternately changing their direction. They are fixed by virtue of two zones of electrostatic interactions of the type (-δ)O-P-O(δ-)...+N(CH3)3 and are more or less parallel to the bc plane. The structure also contains three-layer domains formed by cholesterol molecules perpendicular to isobutylphosphocholine bands. In the direction of the c-axis isobutylphosphocholine bands alternate with the layers of cholesterol molecules herewith reproducing repeated blocks. The obtained structure is compared with that of crystals of phospholipids and cholesterol and its derivatives. Copyright (C) 2000 Elsevier Science B.V.

KW - Biomembrane modeling

KW - Cholesterol

KW - Crystal complex

KW - Phospholipid analog

KW - X-ray analysis

UR - http://www.scopus.com/inward/record.url?scp=0034212246&partnerID=8YFLogxK

U2 - 10.1016/S0005-2736(00)00162-0

DO - 10.1016/S0005-2736(00)00162-0

M3 - Article

C2 - 10825428

AN - SCOPUS:0034212246

VL - 1466

SP - 23

EP - 38

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

SN - 0005-2736

IS - 1-2

ER -

ID: 89841165