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CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system. / Leonova, Elena I.; Reshetnikov, Vasily V. ; Sopova, Julia V. .

In: Research Results in Pharmacology, Vol. 8, No. 3, 13.09.2022, p. 87-98.

Research output: Contribution to journal › Article › peer-review

Harvard

Leonova, EI, Reshetnikov, VV & Sopova, JV 2022, 'CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system', Research Results in Pharmacology, vol. 8, no. 3, pp. 87-98.

APA

Leonova, E. I., Reshetnikov, V. V., & Sopova, J. V. (2022). CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system. Research Results in Pharmacology, 8(3), 87-98.

Vancouver

Leonova EI, Reshetnikov VV, Sopova JV. CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system. Research Results in Pharmacology. 2022 Sep 13;8(3):87-98.

Author

Leonova, Elena I. ; Reshetnikov, Vasily V. ; Sopova, Julia V. . / CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system. In: Research Results in Pharmacology. 2022 ; Vol. 8, No. 3. pp. 87-98.

BibTeX

@article{632f9022cd9b4a2892a6058ae2de00ea,
title = "CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system",
abstract = "Novel CRISPR-Cas-based genome editing tools made it feasible to introduce a variety of precise genomic modifications in the pig genome, including introducing multiple edits simultaneously, inserting long DNA sequences into specifically targeted loci, and performing nucleotide transitions and transversions. Pigs serve as a vital agricultural resource and animal model in biomedical studies, given their advantages over the other models. Pigs share high similarities to humans regarding body/organ size, anatomy, physiology, and a metabolic profile. The pig genome can be modified to carry the same genetic mutations found in humans to replicate inherited diseases to provide preclinical trials of drugs. Moreover, CRISPR-based modification of pigs antigen profile makes it possible to offer porcine organs for xenotransplantation with minimal transplant rejection responses. This review summarizes recent advances in endonuclease-mediated genome editing tools and research progress of genome-edited pigs as personalized test-systems for preclinical trials and as donors of organs with human-fit antigen profile.",
keywords = "CRISPR-CAS, PERSONALIZED MODELS, xenotransplantation, pig, BLASTOCYST COMPLEMENTATION, animal model",
author = "Leonova, {Elena I.} and Reshetnikov, {Vasily V.} and Sopova, {Julia V.}",
year = "2022",
month = sep,
day = "13",
language = "English",
volume = "8",
pages = "87--98",
journal = "Research Results in Pharmacology",
issn = "2658-381X",
publisher = "Белгородский государственный национальный исследовательский университет",
number = "3",

}

RIS

TY - JOUR

T1 - CRISPR/Cas-edited pigs for personalized medicine: more than preclinical test-system

AU - Leonova, Elena I.

AU - Reshetnikov, Vasily V.

AU - Sopova, Julia V.

PY - 2022/9/13

Y1 - 2022/9/13

N2 - Novel CRISPR-Cas-based genome editing tools made it feasible to introduce a variety of precise genomic modifications in the pig genome, including introducing multiple edits simultaneously, inserting long DNA sequences into specifically targeted loci, and performing nucleotide transitions and transversions. Pigs serve as a vital agricultural resource and animal model in biomedical studies, given their advantages over the other models. Pigs share high similarities to humans regarding body/organ size, anatomy, physiology, and a metabolic profile. The pig genome can be modified to carry the same genetic mutations found in humans to replicate inherited diseases to provide preclinical trials of drugs. Moreover, CRISPR-based modification of pigs antigen profile makes it possible to offer porcine organs for xenotransplantation with minimal transplant rejection responses. This review summarizes recent advances in endonuclease-mediated genome editing tools and research progress of genome-edited pigs as personalized test-systems for preclinical trials and as donors of organs with human-fit antigen profile.

AB - Novel CRISPR-Cas-based genome editing tools made it feasible to introduce a variety of precise genomic modifications in the pig genome, including introducing multiple edits simultaneously, inserting long DNA sequences into specifically targeted loci, and performing nucleotide transitions and transversions. Pigs serve as a vital agricultural resource and animal model in biomedical studies, given their advantages over the other models. Pigs share high similarities to humans regarding body/organ size, anatomy, physiology, and a metabolic profile. The pig genome can be modified to carry the same genetic mutations found in humans to replicate inherited diseases to provide preclinical trials of drugs. Moreover, CRISPR-based modification of pigs antigen profile makes it possible to offer porcine organs for xenotransplantation with minimal transplant rejection responses. This review summarizes recent advances in endonuclease-mediated genome editing tools and research progress of genome-edited pigs as personalized test-systems for preclinical trials and as donors of organs with human-fit antigen profile.

KW - CRISPR-CAS

KW - PERSONALIZED MODELS

KW - xenotransplantation

KW - pig

KW - BLASTOCYST COMPLEMENTATION

KW - animal model

UR - https://www.elibrary.ru/item.asp?id=49507081

UR - https://rrpharmacology.ru/article/83872/

M3 - Article

VL - 8

SP - 87

EP - 98

JO - Research Results in Pharmacology

JF - Research Results in Pharmacology

SN - 2658-381X

IS - 3

ER -

ID: 100074852