Research output: Contribution to journal › Article › peer-review
Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency. / Khorolskaya, Julia I.; Perepletchikova, Daria A.; Zhurenkov, Kirill E.; Kachkin, Daniel V.; Rubel, Aleksandr A.; Blinova, Miralda I.; Михайлова, Наталья Аркадьевна.
In: International Journal of Molecular Sciences, Vol. 24, No. 6, 12.03.2023, p. 5431.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency
AU - Khorolskaya, Julia I.
AU - Perepletchikova, Daria A.
AU - Zhurenkov, Kirill E.
AU - Kachkin, Daniel V.
AU - Rubel, Aleksandr A.
AU - Blinova, Miralda I.
AU - Михайлова, Наталья Аркадьевна
N1 - Khorolskaya, J.I.; Perepletchikova, D.A.; Zhurenkov, K.E.; Kachkin, D.V.; Rubel, A.A.; Blinova, M.I.; Mikhailova, N.A. Corneal Reconstruction with EGFP-Labelled Limbal Mesenchymal Stem Cells in a Rabbit Model of Limbal Stem Cell Deficiency. Int. J. Mol. Sci. 2023, 24, 5431. https://doi.org/10.3390/ijms24065431
PY - 2023/3/12
Y1 - 2023/3/12
N2 - Ocular surface reconstruction is essential for treating corneal epithelial defects and vision recovery. Stem cell-based therapy demonstrates promising results but requires further research to elucidate stem cell survival, growth, and differentiation after transplantation in vivo. This study examined the corneal reconstruction promoted by EGFP-labeled limbal mesenchymal stem cells (L-MSCs-EGFP) and their fate after transplantation. EGFP labeling allowed us to evaluate the migration and survival rates of the transferred cells. L-MSCs-EGFP seeded onto decellularized human amniotic membrane (dHAM) were transplanted into rabbits with a modeled limbal stem cell deficiency. The localization and viability of the transplanted cells in animal tissue were analyzed using histology, immunohistochemistry, and confocal microscopy up to 3 months after transplantation. EGFP-labeled cells remained viable for the first 14 days after transplantation. By the 90th day, epithelialization of the rabbit corneas reached 90%, but the presence of viable labeled cells was not observed within the newly formed epithelium. Although labeled cells demonstrated low survivability in host tissue, the squamous corneal-like epithelium was partially restored by the 30th day after transplantation of the tissue-engineered graft. Overall, this study paves the way for further optimization of transplantation conditions and studying the mechanisms of corneal tissue restoration.
AB - Ocular surface reconstruction is essential for treating corneal epithelial defects and vision recovery. Stem cell-based therapy demonstrates promising results but requires further research to elucidate stem cell survival, growth, and differentiation after transplantation in vivo. This study examined the corneal reconstruction promoted by EGFP-labeled limbal mesenchymal stem cells (L-MSCs-EGFP) and their fate after transplantation. EGFP labeling allowed us to evaluate the migration and survival rates of the transferred cells. L-MSCs-EGFP seeded onto decellularized human amniotic membrane (dHAM) were transplanted into rabbits with a modeled limbal stem cell deficiency. The localization and viability of the transplanted cells in animal tissue were analyzed using histology, immunohistochemistry, and confocal microscopy up to 3 months after transplantation. EGFP-labeled cells remained viable for the first 14 days after transplantation. By the 90th day, epithelialization of the rabbit corneas reached 90%, but the presence of viable labeled cells was not observed within the newly formed epithelium. Although labeled cells demonstrated low survivability in host tissue, the squamous corneal-like epithelium was partially restored by the 30th day after transplantation of the tissue-engineered graft. Overall, this study paves the way for further optimization of transplantation conditions and studying the mechanisms of corneal tissue restoration.
KW - клетки, меченные GFP
KW - глазная клеточная терапия
KW - регенерация роговицы
KW - регенеративная офтальмология
KW - лимбальные мезенхимальные стволовые клетки
KW - GFP-labeled cells
KW - ocular cell therapy
KW - cornea regeneration
KW - regenerative ophthalmology
KW - mesenchymal stem cells
KW - Rabbits
KW - Cornea
KW - Humans
KW - Cells, Cultured
KW - Epithelium, Corneal/metabolism
KW - Mesenchymal Stem Cells
KW - Limbus Corneae
KW - Limbal Stem Cells
KW - Stem Cell Transplantation
KW - Animals
UR - https://www.mendeley.com/catalogue/2552fa2a-5310-34c7-8d61-d72a2e6ad8bd/
U2 - 10.3390/ijms24065431
DO - 10.3390/ijms24065431
M3 - Article
C2 - 36982507
VL - 24
SP - 5431
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1422-0067
IS - 6
ER -
ID: 103479725