In this study, we examined the influence of long-term conventional tillage on the water-physical, chemical properties, and composition of the organic matter of chernozems. The study has been conducted on an arable plot subjected to water and wind erosion in the Pre-Ural steppe zone (Republic of Bashkortostan, Russia). Soil samples were collected from non-eroded and eroded arable plots as well as from an adjacent pristine forest windbreak. Key structural fragments of soil organic matter under different land use types were identified and quantified using ¹³C-NMR spectroscopy. The results showed that the water-physical properties deteriorated in agrochernozems: the number of valuable soil aggregates decreased and the soil bulk density increased, which may limit the growth of crops. The soil organic matter content for the different samples varied in the following direction: arable non-eroded > forest windbreak > arable eroded. It has been found that long-term plowing by conventional methods decreases aliphatic and increases aromatic structures in soils. As a result of the reduced inputs of plant residues, the processes of humification slowed down compared to unplowed soils. To increase soil fertility and carbon sequestration potential, it is necessary to stop degradation processes and implement conservation tillage practices.