TY - JOUR

T1 - Contrast Relative Humidity Response of Diverse Cowpea (Vigna unguiculata (L.) Walp.) Genotypes: Deep Study Using RNAseq Approach

AU - Krylova, E.A.

AU - Burlyaeva, M.O.

AU - Tvorogova, V.E.

AU - Khlestkina, E.K.

N1 - Export Date: 4 November 2024

PY - 2024/10/15

Y1 - 2024/10/15

N2 - Cowpea (Vigna unguiculata (L.) Walp.) is appreciated for its suitability for cultivation and obtaining good yields in relatively extreme farming conditions. It is resistant to high temperatures and drought. Moreover, food products prepared from Vigna are rich in many nutrients such as proteins, amino acids, carbohydrates, minerals, fiber, vitamins, and other bioactive compounds. However, in East and Southeast Asia, where the products of this crop are in demand, the climate is characterized by excessive humidity. Under these conditions, the vast majority of cowpea varieties tend to have indeterminate growth (elongated shoot length) and are unsuitable for mechanized harvesting. The molecular mechanisms for tolerance to high relative humidity remain the least studied in comparison with those for other abiotic stress factors (drought, heat, cold, flooding, etc.). The purpose of the work was to reveal and investigate differentially expressed genes in cowpea accessions having contrasting growth habits (determinate and indeterminate) under humid and drought conditions. We performed RNA-seq analysis using selected cowpea accessions from the VIR collection. Among the genotypes used, some have significant changes in their plant architecture in response to high relative humidity, while others were tolerant to these conditions. In total, we detected 1697 upregulated and 1933 downregulated genes. The results showed that phytohormone-related genes are involved in cowpea response to high relative humidity. DEGs associated with jasmonic acid signaling are proposed to be key contributors in the maintenance of compact architecture under humid conditions. © 2024 by the authors.

AB - Cowpea (Vigna unguiculata (L.) Walp.) is appreciated for its suitability for cultivation and obtaining good yields in relatively extreme farming conditions. It is resistant to high temperatures and drought. Moreover, food products prepared from Vigna are rich in many nutrients such as proteins, amino acids, carbohydrates, minerals, fiber, vitamins, and other bioactive compounds. However, in East and Southeast Asia, where the products of this crop are in demand, the climate is characterized by excessive humidity. Under these conditions, the vast majority of cowpea varieties tend to have indeterminate growth (elongated shoot length) and are unsuitable for mechanized harvesting. The molecular mechanisms for tolerance to high relative humidity remain the least studied in comparison with those for other abiotic stress factors (drought, heat, cold, flooding, etc.). The purpose of the work was to reveal and investigate differentially expressed genes in cowpea accessions having contrasting growth habits (determinate and indeterminate) under humid and drought conditions. We performed RNA-seq analysis using selected cowpea accessions from the VIR collection. Among the genotypes used, some have significant changes in their plant architecture in response to high relative humidity, while others were tolerant to these conditions. In total, we detected 1697 upregulated and 1933 downregulated genes. The results showed that phytohormone-related genes are involved in cowpea response to high relative humidity. DEGs associated with jasmonic acid signaling are proposed to be key contributors in the maintenance of compact architecture under humid conditions. © 2024 by the authors.

KW - cowpea

KW - differentially expressed gene

KW - high relative humidity

KW - transcriptomics

KW - RNA-Seq/methods

KW - Gene Expression Profiling/methods

KW - Vigna/genetics

KW - Genotype

KW - Transcriptome/genetics

KW - Stress, Physiological/genetics

KW - Droughts

KW - Plant Proteins/genetics

KW - Humidity

KW - Gene Expression Regulation, Plant

KW - Sequence Analysis, RNA/methods

UR - https://www.mendeley.com/catalogue/481f8d10-745c-3479-a585-bf030e798cab/

U2 - 10.3390/ijms252011056

DO - 10.3390/ijms252011056

M3 - статья

C2 - 39456837

VL - 25

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 20

ER -