Standard

Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System. / Matveenko, A. G.; Mikhailichenko, A. S.; Zhouravleva, G. A.

In: Microbiology, Vol. 93, No. 2, 01.04.2024, p. 154-159.

Research output: Contribution to journal › Article › peer-review

Harvard

Matveenko, AG, Mikhailichenko, AS & Zhouravleva, GA 2024, 'Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System', Microbiology, vol. 93, no. 2, pp. 154-159. https://doi.org/10.1134/s0026261723604256

APA

Matveenko, A. G., Mikhailichenko, A. S., & Zhouravleva, G. A. (2024). Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System. Microbiology, 93(2), 154-159. https://doi.org/10.1134/s0026261723604256

Vancouver

Matveenko AG, Mikhailichenko AS, Zhouravleva GA. Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System. Microbiology. 2024 Apr 1;93(2):154-159. https://doi.org/10.1134/s0026261723604256

Author

Matveenko, A. G. ; Mikhailichenko, A. S. ; Zhouravleva, G. A. / Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System. In: Microbiology. 2024 ; Vol. 93, No. 2. pp. 154-159.

BibTeX

@article{135abc2d88e54b80bd1c7dedc48c9fb8,
title = "Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System",
abstract = "Abstract: New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.",
keywords = "CRISPR/Cas9, SUP35, Saccharomyces cerevisiae, genome editing",
author = "Matveenko, {A. G.} and Mikhailichenko, {A. S.} and Zhouravleva, {G. A.}",
year = "2024",
month = apr,
day = "1",
doi = "10.1134/s0026261723604256",
language = "English",
volume = "93",
pages = "154--159",
journal = "МИКРОБИОЛОГИЯ",
issn = "0026-3656",
publisher = "Издательство {"}Наука{"}",
number = "2",

}

RIS

TY - JOUR

T1 - Construction of Vectors for the Genome Editing of Saccharomyces Yeast Using CRISPR-Cas9 System

AU - Matveenko, A. G.

AU - Mikhailichenko, A. S.

AU - Zhouravleva, G. A.

PY - 2024/4/1

Y1 - 2024/4/1

N2 - Abstract: New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.

AB - Abstract: New vectors for the yeast genome editing using CRISPR/Cas9 were constructed. A system for cloning of new targets using the standard methods (PCR‒restriction‒ligation) was developed and successfully applied. The constructed vectors allowed us to obtain the sup35-25 mutants, deletion of the PSH1 gene and disruption of the NAM7 (UPF1). A convenient method for identifying plasmids with a new target was tested. A detailed description of the cloning technique used and selection of plasmids with the new targets is provided.

KW - CRISPR/Cas9

KW - SUP35

KW - Saccharomyces cerevisiae

KW - genome editing

UR - https://www.mendeley.com/catalogue/b726aff0-b029-32a5-b980-01b591860ad2/

U2 - 10.1134/s0026261723604256

DO - 10.1134/s0026261723604256

M3 - Article

VL - 93

SP - 154

EP - 159

JO - МИКРОБИОЛОГИЯ

JF - МИКРОБИОЛОГИЯ

SN - 0026-3656

IS - 2

ER -

ID: 127488077