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Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma. / Abakumova, Tatiana; Abakumov, Maxim; Shein, Sergey; Chelushkin, Pavel; Bychkov, Dmitry; Mukhin, Vladimir; Yusubalieva, Gaukhar; Grinenko, Nadezhda; Kabanov, Alexander; Nukolova, Natalia; Chekhonin, Vladimir.

In: Contrast Media and Molecular Imaging, Vol. 11, No. 1, 01.01.2016, p. 15-23.

Research output: Contribution to journal › Article › peer-review

Harvard

Abakumova, T, Abakumov, M, Shein, S, Chelushkin, P, Bychkov, D, Mukhin, V, Yusubalieva, G, Grinenko, N, Kabanov, A, Nukolova, N & Chekhonin, V 2016, 'Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma', Contrast Media and Molecular Imaging, vol. 11, no. 1, pp. 15-23. https://doi.org/10.1002/cmmi.1653

APA

Abakumova, T., Abakumov, M., Shein, S., Chelushkin, P., Bychkov, D., Mukhin, V., Yusubalieva, G., Grinenko, N., Kabanov, A., Nukolova, N., & Chekhonin, V. (2016). Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma. Contrast Media and Molecular Imaging, 11(1), 15-23. https://doi.org/10.1002/cmmi.1653

Vancouver

Abakumova T, Abakumov M, Shein S, Chelushkin P, Bychkov D, Mukhin V et al. Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma. Contrast Media and Molecular Imaging. 2016 Jan 1;11(1):15-23. https://doi.org/10.1002/cmmi.1653

Author

Abakumova, Tatiana ; Abakumov, Maxim ; Shein, Sergey ; Chelushkin, Pavel ; Bychkov, Dmitry ; Mukhin, Vladimir ; Yusubalieva, Gaukhar ; Grinenko, Nadezhda ; Kabanov, Alexander ; Nukolova, Natalia ; Chekhonin, Vladimir. / Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma. In: Contrast Media and Molecular Imaging. 2016 ; Vol. 11, No. 1. pp. 15-23.

BibTeX

@article{57697b7dbb1e4c948e7407c169496c88,
title = "Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma",
abstract = "Glioblastoma multiforme is the most aggressive form of brain tumor. Early and accurate diagnosis of glioma and its borders is an important step for its successful treatment. One of the promising targets for selective visualization of glioma and its margins is connexin 43 (Cx43), which is highly expressed in reactive astrocytes and migrating glioma cells. The purpose of this study was to synthesize a Gd-based contrast agent conjugated with specific antibodies to Cx43 for efficient visualization of glioma C6 in vivo. We have prepared stable nontoxic conjugates of monoclonal antibody to Cx43 and polylysine-DTPA ligands complexed with Gd(III), which are characterized by higher T1 relaxivity (6.5 mM-1 s-1 at 7 T) than the commercial agent Magnevist{\textregistered} (3.4 mM-1 s-1). Cellular uptake of Cx43-specific T1 contrast agent in glioma C6 cells was more than four times higher than the nonspecific IgG-contrast agent, as detected by flow cytometry and confocal analysis. MRI experiments showed that the obtained agents could markedly enhance visualization of glioma C6 in vivo after their intravenous administration. Significant accumulation of Cx43-targeted contrast agents in glioma and the peritumoral zone led not only to enhanced contrast but also to improved detection of the tumor periphery. Fluorescence imaging confirmed notable accumulation of Cx43-specific conjugates in the peritumoral zone compared with nonspecific IgG conjugates at 24 h after intravenous injection. All these features of Cx43-targeted contrast agents might be useful for more precise diagnosis of glioma and its borders by MRI.",
keywords = "Cellular targeting, Connexin 43, Glioma, MRI, T contrast agent, Targeted imaging",
author = "Tatiana Abakumova and Maxim Abakumov and Sergey Shein and Pavel Chelushkin and Dmitry Bychkov and Vladimir Mukhin and Gaukhar Yusubalieva and Nadezhda Grinenko and Alexander Kabanov and Natalia Nukolova and Vladimir Chekhonin",
year = "2016",
month = jan,
day = "1",
doi = "10.1002/cmmi.1653",
language = "English",
volume = "11",
pages = "15--23",
journal = "Contrast Media and Molecular Imaging",
issn = "1555-4309",
publisher = "Hindawi ",
number = "1",

}

RIS

TY - JOUR

T1 - Connexin 43-targeted T1 contrast agent for MRI diagnosis of glioma

AU - Abakumova, Tatiana

AU - Abakumov, Maxim

AU - Shein, Sergey

AU - Chelushkin, Pavel

AU - Bychkov, Dmitry

AU - Mukhin, Vladimir

AU - Yusubalieva, Gaukhar

AU - Grinenko, Nadezhda

AU - Kabanov, Alexander

AU - Nukolova, Natalia

AU - Chekhonin, Vladimir

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Glioblastoma multiforme is the most aggressive form of brain tumor. Early and accurate diagnosis of glioma and its borders is an important step for its successful treatment. One of the promising targets for selective visualization of glioma and its margins is connexin 43 (Cx43), which is highly expressed in reactive astrocytes and migrating glioma cells. The purpose of this study was to synthesize a Gd-based contrast agent conjugated with specific antibodies to Cx43 for efficient visualization of glioma C6 in vivo. We have prepared stable nontoxic conjugates of monoclonal antibody to Cx43 and polylysine-DTPA ligands complexed with Gd(III), which are characterized by higher T1 relaxivity (6.5 mM-1 s-1 at 7 T) than the commercial agent Magnevist® (3.4 mM-1 s-1). Cellular uptake of Cx43-specific T1 contrast agent in glioma C6 cells was more than four times higher than the nonspecific IgG-contrast agent, as detected by flow cytometry and confocal analysis. MRI experiments showed that the obtained agents could markedly enhance visualization of glioma C6 in vivo after their intravenous administration. Significant accumulation of Cx43-targeted contrast agents in glioma and the peritumoral zone led not only to enhanced contrast but also to improved detection of the tumor periphery. Fluorescence imaging confirmed notable accumulation of Cx43-specific conjugates in the peritumoral zone compared with nonspecific IgG conjugates at 24 h after intravenous injection. All these features of Cx43-targeted contrast agents might be useful for more precise diagnosis of glioma and its borders by MRI.

AB - Glioblastoma multiforme is the most aggressive form of brain tumor. Early and accurate diagnosis of glioma and its borders is an important step for its successful treatment. One of the promising targets for selective visualization of glioma and its margins is connexin 43 (Cx43), which is highly expressed in reactive astrocytes and migrating glioma cells. The purpose of this study was to synthesize a Gd-based contrast agent conjugated with specific antibodies to Cx43 for efficient visualization of glioma C6 in vivo. We have prepared stable nontoxic conjugates of monoclonal antibody to Cx43 and polylysine-DTPA ligands complexed with Gd(III), which are characterized by higher T1 relaxivity (6.5 mM-1 s-1 at 7 T) than the commercial agent Magnevist® (3.4 mM-1 s-1). Cellular uptake of Cx43-specific T1 contrast agent in glioma C6 cells was more than four times higher than the nonspecific IgG-contrast agent, as detected by flow cytometry and confocal analysis. MRI experiments showed that the obtained agents could markedly enhance visualization of glioma C6 in vivo after their intravenous administration. Significant accumulation of Cx43-targeted contrast agents in glioma and the peritumoral zone led not only to enhanced contrast but also to improved detection of the tumor periphery. Fluorescence imaging confirmed notable accumulation of Cx43-specific conjugates in the peritumoral zone compared with nonspecific IgG conjugates at 24 h after intravenous injection. All these features of Cx43-targeted contrast agents might be useful for more precise diagnosis of glioma and its borders by MRI.

KW - Cellular targeting

KW - Connexin 43

KW - Glioma

KW - MRI

KW - T contrast agent

KW - Targeted imaging

UR - http://www.scopus.com/inward/record.url?scp=84956585683&partnerID=8YFLogxK

U2 - 10.1002/cmmi.1653

DO - 10.1002/cmmi.1653

M3 - Article

C2 - 26265140

AN - SCOPUS:84956585683

VL - 11

SP - 15

EP - 23

JO - Contrast Media and Molecular Imaging

JF - Contrast Media and Molecular Imaging

SN - 1555-4309

IS - 1

ER -

ID: 48335057