Research output: Contribution to journal › Article › peer-review
Conformational properties of biocompatible poly(2-ethyl-2-oxazoline)s in phosphate buffered saline. / Gubarev, Alexander S.; Monnery, Bryn D.; Lezov, Alexey A.; Sedlacek, Ondrej; Tsvetkov, Nikolai V.; Hoogenboom, Richard; Filippov, Sergey K.
In: Polymer Chemistry, Vol. 9, No. 17, 07.05.2018, p. 2232-2237.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Conformational properties of biocompatible poly(2-ethyl-2-oxazoline)s in phosphate buffered saline
AU - Gubarev, Alexander S.
AU - Monnery, Bryn D.
AU - Lezov, Alexey A.
AU - Sedlacek, Ondrej
AU - Tsvetkov, Nikolai V.
AU - Hoogenboom, Richard
AU - Filippov, Sergey K.
PY - 2018/5/7
Y1 - 2018/5/7
N2 - Inspired by the increasing popularity of poly(2-ethyl-2-oxazoline) (PEtOx) for biomedical applications, this study reports the complete and thorough solution analysis of the homologous series of biocompatible PEtOx samples in a very broad range of molecular weights ranging from 11.2 x 10(3) g mol(-1) up to 260 x 10(3) g mol(-1). The main focus of the research was on the determination of the conformational properties of PEtOx macromolecules at a temperature of 37 degrees C in phosphate buffered saline (PBS) simulating the parameters of physiological media. The polymers were studied in PBS solutions by analytical ultracentrifugation, dynamic light scattering (DLS), translational diffusion, and intrinsic viscosity measurements in a temperature range from 15 degrees C up to 72 degrees C. The complete set of Kuhn-Mark-Houwink-Sakurada scaling relationships revealed linear trends over the whole range of the studied molar masses, while the determined scaling indices at 37 degrees C correspond to the coil conformation in a thermodynamically good solvent ([eta] = 0.045 x M-0.62, s(0) = 0.010 x M-0.46 and D-0 = 1750 x M-0.54). Based on the intrinsic viscosity values (most sensitive characteristic to the size variations of polymer coils, [eta] similar to r(3)), it was demonstrated that PEtOx macromolecules in PBS solutions undergo a transition from swollen polymer coils with gradual deterioration of thermodynamic quality of solutions within the temperature range of 15-45 degrees C, reaching theta-conditions at 55 degrees C with further precipitation at 62-72 degrees C. Also, to the best of our knowledge, the conformational parameters (equilibrium rigidity/the Kuhn segment length and the diameter of the polymer chain) of PEtOx macromolecules were evaluated under physiological conditions for the first time and constitute A = 1.8 +/- 0.3 nm and d = 0.7 +/- 0.4 nm. These equilibrium rigidity values classify PEtOx as a flexible macromolecule with rigidity similar to that of poly(ethylene glycol). For the first time, we were able to demonstrate a direct influence of thermosensitivity on the rigidity of the biocompatible polymer: PEtOx. The Kuhn segment length is undoubtedly decreasing when approaching the LCST.
AB - Inspired by the increasing popularity of poly(2-ethyl-2-oxazoline) (PEtOx) for biomedical applications, this study reports the complete and thorough solution analysis of the homologous series of biocompatible PEtOx samples in a very broad range of molecular weights ranging from 11.2 x 10(3) g mol(-1) up to 260 x 10(3) g mol(-1). The main focus of the research was on the determination of the conformational properties of PEtOx macromolecules at a temperature of 37 degrees C in phosphate buffered saline (PBS) simulating the parameters of physiological media. The polymers were studied in PBS solutions by analytical ultracentrifugation, dynamic light scattering (DLS), translational diffusion, and intrinsic viscosity measurements in a temperature range from 15 degrees C up to 72 degrees C. The complete set of Kuhn-Mark-Houwink-Sakurada scaling relationships revealed linear trends over the whole range of the studied molar masses, while the determined scaling indices at 37 degrees C correspond to the coil conformation in a thermodynamically good solvent ([eta] = 0.045 x M-0.62, s(0) = 0.010 x M-0.46 and D-0 = 1750 x M-0.54). Based on the intrinsic viscosity values (most sensitive characteristic to the size variations of polymer coils, [eta] similar to r(3)), it was demonstrated that PEtOx macromolecules in PBS solutions undergo a transition from swollen polymer coils with gradual deterioration of thermodynamic quality of solutions within the temperature range of 15-45 degrees C, reaching theta-conditions at 55 degrees C with further precipitation at 62-72 degrees C. Also, to the best of our knowledge, the conformational parameters (equilibrium rigidity/the Kuhn segment length and the diameter of the polymer chain) of PEtOx macromolecules were evaluated under physiological conditions for the first time and constitute A = 1.8 +/- 0.3 nm and d = 0.7 +/- 0.4 nm. These equilibrium rigidity values classify PEtOx as a flexible macromolecule with rigidity similar to that of poly(ethylene glycol). For the first time, we were able to demonstrate a direct influence of thermosensitivity on the rigidity of the biocompatible polymer: PEtOx. The Kuhn segment length is undoubtedly decreasing when approaching the LCST.
KW - DILUTE-SOLUTION
KW - LIGHT-SCATTERING
KW - INTRINSIC-VISCOSITY
KW - MOLECULAR-WEIGHT
KW - IN-VITRO
KW - POLY(2-OXAZOLINE)S
KW - POLY(N-ISOPROPYLACRYLAMIDE)
KW - CANCER
KW - CHAINS
KW - LCST
UR - http://www.scopus.com/inward/record.url?scp=85046263644&partnerID=8YFLogxK
UR - http://www.mendeley.com/research/conformational-properties-biocompatible-poly2ethyl2oxazolines-phosphate-buffered-saline
U2 - 10.1039/c8py00255j
DO - 10.1039/c8py00255j
M3 - Article
AN - SCOPUS:85046263644
VL - 9
SP - 2232
EP - 2237
JO - Polymer Chemistry
JF - Polymer Chemistry
SN - 1759-9954
IS - 17
ER -
ID: 36420006