Composition of intracellular and cell wall-bound phlorotannin fractions in fucoid algae indicates specific functions of these metabolites dependent on the chemical structure

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Composition of intracellular and cell wall-bound phlorotannin fractions in fucoid algae indicates specific functions of these metabolites dependent on the chemical structure. / Birkemeyer, Claudia; Lemesheva, Valeriya ; Billig, Susan; Tarakhovskaya, Elena .

In: Metabolites, Vol. 10, No. 9, 369, 11.09.2020, p. 1-20.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{90437b81e8f54925b815a2d4bcf39ffa,

title = "Composition of intracellular and cell wall-bound phlorotannin fractions in fucoid algae indicates specific functions of these metabolites dependent on the chemical structure",

abstract = "Accumulation of biologically active metabolites is a specific feature of plant biochemistry, directing the use of plants in numerous applications in the pharmaceutical and food industries. Among these substances, the plethora of phenolic compounds has attracted particular interest among researchers. Here, we report on new findings in phlorotannin research, a large group of multifunctional phenolic substances, produced in brown algae. Comprehensive LC-MS profiling of three algal species allowed us to depict the complex pattern of this structurally diverse compound group across different tissues and subcellular compartments. We compiled more than 30 different phlorotannin series in one sample and used accurate mass spectrometry to assign tentative structures to the observed ions based on the confirmed sum formulas. From that, we found that acetylation, hydroxylation, and oxidation are likely to be the most common in vivo modifications to phlorotannins. Using an alternative data mining strategy to cope with extensive coelution and structural isomers, we quantitatively compared the intensity of different phlorotannin series in species, tissues, and subcellular compartments to learn more about their physiological functions. The structure and intra-thallus profiles of cell wall-bound phlorotannins were studied here for the first time. We suggest that one of the major dibenzodioxin-type phlorotannin series may exclusively target integration into the cell wall of fucoid algae. ",

keywords = "Acetylation, Brown algae, cell wall, Fucus, LC-MS profiling, Pelvetia, phlorotannin fingerprint, Physodes, physodes, brown algae, ANTIOXIDANT ACTIVITY, CHROMATOGRAPHIC ANALYSIS, MOLECULAR-WEIGHT, PHENOLIC-COMPOUNDS, INHIBITORY-ACTIVITY, SUBSTANCES, PHAEOPHYCEAE, acetylation, FUCUS-VESICULOSUS, BROWN, MACROALGAE",

author = "Claudia Birkemeyer and Valeriya Lemesheva and Susan Billig and Elena Tarakhovskaya",

note = "Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = sep,

day = "11",

doi = "10.3390/metabo10090369",

language = "English",

volume = "10",

pages = "1--20",

journal = "Metabolites",

issn = "2218-1989",

publisher = "MDPI AG",

number = "9",

}

RIS

TY - JOUR

T1 - Composition of intracellular and cell wall-bound phlorotannin fractions in fucoid algae indicates specific functions of these metabolites dependent on the chemical structure

AU - Birkemeyer, Claudia

AU - Lemesheva, Valeriya

AU - Billig, Susan

AU - Tarakhovskaya, Elena

PY - 2020/9/11

Y1 - 2020/9/11

N2 - Accumulation of biologically active metabolites is a specific feature of plant biochemistry, directing the use of plants in numerous applications in the pharmaceutical and food industries. Among these substances, the plethora of phenolic compounds has attracted particular interest among researchers. Here, we report on new findings in phlorotannin research, a large group of multifunctional phenolic substances, produced in brown algae. Comprehensive LC-MS profiling of three algal species allowed us to depict the complex pattern of this structurally diverse compound group across different tissues and subcellular compartments. We compiled more than 30 different phlorotannin series in one sample and used accurate mass spectrometry to assign tentative structures to the observed ions based on the confirmed sum formulas. From that, we found that acetylation, hydroxylation, and oxidation are likely to be the most common in vivo modifications to phlorotannins. Using an alternative data mining strategy to cope with extensive coelution and structural isomers, we quantitatively compared the intensity of different phlorotannin series in species, tissues, and subcellular compartments to learn more about their physiological functions. The structure and intra-thallus profiles of cell wall-bound phlorotannins were studied here for the first time. We suggest that one of the major dibenzodioxin-type phlorotannin series may exclusively target integration into the cell wall of fucoid algae.

AB - Accumulation of biologically active metabolites is a specific feature of plant biochemistry, directing the use of plants in numerous applications in the pharmaceutical and food industries. Among these substances, the plethora of phenolic compounds has attracted particular interest among researchers. Here, we report on new findings in phlorotannin research, a large group of multifunctional phenolic substances, produced in brown algae. Comprehensive LC-MS profiling of three algal species allowed us to depict the complex pattern of this structurally diverse compound group across different tissues and subcellular compartments. We compiled more than 30 different phlorotannin series in one sample and used accurate mass spectrometry to assign tentative structures to the observed ions based on the confirmed sum formulas. From that, we found that acetylation, hydroxylation, and oxidation are likely to be the most common in vivo modifications to phlorotannins. Using an alternative data mining strategy to cope with extensive coelution and structural isomers, we quantitatively compared the intensity of different phlorotannin series in species, tissues, and subcellular compartments to learn more about their physiological functions. The structure and intra-thallus profiles of cell wall-bound phlorotannins were studied here for the first time. We suggest that one of the major dibenzodioxin-type phlorotannin series may exclusively target integration into the cell wall of fucoid algae.

KW - Acetylation

KW - Brown algae

KW - cell wall

KW - Fucus

KW - LC-MS profiling

KW - Pelvetia

KW - phlorotannin fingerprint

KW - Physodes

KW - physodes

KW - brown algae

KW - ANTIOXIDANT ACTIVITY

KW - CHROMATOGRAPHIC ANALYSIS

KW - MOLECULAR-WEIGHT

KW - PHENOLIC-COMPOUNDS

KW - INHIBITORY-ACTIVITY

KW - SUBSTANCES

KW - PHAEOPHYCEAE

KW - acetylation

KW - FUCUS-VESICULOSUS

KW - BROWN

KW - MACROALGAE

UR - http://www.scopus.com/inward/record.url?scp=85092183717&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/0a7ff01d-d9fb-3a8e-8116-f761695dbf97/

U2 - 10.3390/metabo10090369

DO - 10.3390/metabo10090369

M3 - Article

VL - 10

SP - 1

EP - 20

JO - Metabolites

JF - Metabolites

SN - 2218-1989

IS - 9

M1 - 369

ER -

ID: 69956263