Complex life cycle, broad host range and adaptation strategy of the intranuclear Paramecium symbiont Preeria caryophila comb. nov.

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Complex life cycle, broad host range and adaptation strategy of the intranuclear Paramecium symbiont Preeria caryophila comb. nov. / Potekhin, Alexey; Schweikert, Michael; Nekrasova, Irina; Vitali, Valerio; Schwarzer, Sabine; Anikina, Arina; Kaltz, Oliver; Petroni, Giulio; Schrallhammer, Martina.

In: FEMS Microbiology Ecology, Vol. 94, No. 7, fiy076, 01.07.2018.

Research output: Contribution to journal › Article › peer-review

Author

Potekhin, Alexey ; Schweikert, Michael ; Nekrasova, Irina ; Vitali, Valerio ; Schwarzer, Sabine ; Anikina, Arina ; Kaltz, Oliver ; Petroni, Giulio ; Schrallhammer, Martina. / Complex life cycle, broad host range and adaptation strategy of the intranuclear Paramecium symbiont Preeria caryophila comb. nov. In: FEMS Microbiology Ecology. 2018 ; Vol. 94, No. 7.

BibTeX

@article{98f33de4b33841f6a07fee21b590b5a7,

title = "Complex life cycle, broad host range and adaptation strategy of the intranuclear Paramecium symbiont Preeria caryophila comb. nov.",

abstract = "Holospora and related bacteria are a group of obligate Paramecium symbionts. Characteristic features are their infectivity, the presence of two distinct morphotypes, and usually a strict specialization for a single Paramecium species as host and for a nuclear compartment (either somatic or generative nucleus) for reproduction. Holospora caryophila steps out of line, naturally occurring in Paramecium biaurelia and Paramecium caudatum. This study addresses the phylogenetic relationship among H. caryophila and other Holospora species based on 16S rRNA gene sequence comparison analyzing the type strain and seven new macronuclear symbionts. Key aspects of Holospora physiology such as infectivity, symbiosis establishment and host range were determined by comprehensive infection assays. Detailed morphological investigations and sequence-based phylogeny confirmed a high similarity between the type strain of H. caryophila and the novel strains. Surprisingly, they are only distantly related to other Holospora species suggesting that they belong to a new genus within the family Holosporaceae, here described as Preeria caryophila comb. nov. Adding to this phylogenetic distance, we also observed a much broader host range, comprising at least eleven Paramecium species. As these potential host species exhibit substantial differences in frequency of sexual processes, P. caryophila demonstrates which adaptations are crucial for macronuclear symbionts facing regular destruction of their habitat.",

keywords = "Bacterial infection, Ciliate, Endosymbiosis, Holospora, Holosporales, Nuclear symbiont, BURSARIA, MICRONUCLEUS, ENDOSYMBIONTS, INFECTIVITY, bacterial infection, nuclear symbiont, TRANSMISSION, CAUDATUM, BACTERIUM HOLOSPORA-ELEGANS, endosymbiosis, ciliate, OBTUSA, MACRONUCLEAR SYMBIONT, GROWTH-CONDITIONS",

author = "Alexey Potekhin and Michael Schweikert and Irina Nekrasova and Valerio Vitali and Sabine Schwarzer and Arina Anikina and Oliver Kaltz and Giulio Petroni and Martina Schrallhammer",

year = "2018",

month = jul,

day = "1",

doi = "10.1093/femsec/fiy076",

language = "English",

volume = "94",

journal = "FEMS Microbiology Ecology",

issn = "0168-6496",

publisher = "Oxford University Press",

number = "7",

}

RIS

TY - JOUR

T1 - Complex life cycle, broad host range and adaptation strategy of the intranuclear Paramecium symbiont Preeria caryophila comb. nov.

AU - Potekhin, Alexey

AU - Schweikert, Michael

AU - Nekrasova, Irina

AU - Vitali, Valerio

AU - Schwarzer, Sabine

AU - Anikina, Arina

AU - Kaltz, Oliver

AU - Petroni, Giulio

AU - Schrallhammer, Martina

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Holospora and related bacteria are a group of obligate Paramecium symbionts. Characteristic features are their infectivity, the presence of two distinct morphotypes, and usually a strict specialization for a single Paramecium species as host and for a nuclear compartment (either somatic or generative nucleus) for reproduction. Holospora caryophila steps out of line, naturally occurring in Paramecium biaurelia and Paramecium caudatum. This study addresses the phylogenetic relationship among H. caryophila and other Holospora species based on 16S rRNA gene sequence comparison analyzing the type strain and seven new macronuclear symbionts. Key aspects of Holospora physiology such as infectivity, symbiosis establishment and host range were determined by comprehensive infection assays. Detailed morphological investigations and sequence-based phylogeny confirmed a high similarity between the type strain of H. caryophila and the novel strains. Surprisingly, they are only distantly related to other Holospora species suggesting that they belong to a new genus within the family Holosporaceae, here described as Preeria caryophila comb. nov. Adding to this phylogenetic distance, we also observed a much broader host range, comprising at least eleven Paramecium species. As these potential host species exhibit substantial differences in frequency of sexual processes, P. caryophila demonstrates which adaptations are crucial for macronuclear symbionts facing regular destruction of their habitat.

AB - Holospora and related bacteria are a group of obligate Paramecium symbionts. Characteristic features are their infectivity, the presence of two distinct morphotypes, and usually a strict specialization for a single Paramecium species as host and for a nuclear compartment (either somatic or generative nucleus) for reproduction. Holospora caryophila steps out of line, naturally occurring in Paramecium biaurelia and Paramecium caudatum. This study addresses the phylogenetic relationship among H. caryophila and other Holospora species based on 16S rRNA gene sequence comparison analyzing the type strain and seven new macronuclear symbionts. Key aspects of Holospora physiology such as infectivity, symbiosis establishment and host range were determined by comprehensive infection assays. Detailed morphological investigations and sequence-based phylogeny confirmed a high similarity between the type strain of H. caryophila and the novel strains. Surprisingly, they are only distantly related to other Holospora species suggesting that they belong to a new genus within the family Holosporaceae, here described as Preeria caryophila comb. nov. Adding to this phylogenetic distance, we also observed a much broader host range, comprising at least eleven Paramecium species. As these potential host species exhibit substantial differences in frequency of sexual processes, P. caryophila demonstrates which adaptations are crucial for macronuclear symbionts facing regular destruction of their habitat.

KW - Bacterial infection

KW - Ciliate

KW - Endosymbiosis

KW - Holospora

KW - Holosporales

KW - Nuclear symbiont

KW - BURSARIA

KW - MICRONUCLEUS

KW - ENDOSYMBIONTS

KW - INFECTIVITY

KW - bacterial infection

KW - nuclear symbiont

KW - TRANSMISSION

KW - CAUDATUM

KW - BACTERIUM HOLOSPORA-ELEGANS

KW - endosymbiosis

KW - ciliate

KW - OBTUSA

KW - MACRONUCLEAR SYMBIONT

KW - GROWTH-CONDITIONS

UR - http://www.scopus.com/inward/record.url?scp=85050610447&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/complex-life-cycle-broad-host-range-adaptation-strategy-intranuclear-paramecium-symbiont-preeria-car

U2 - 10.1093/femsec/fiy076

DO - 10.1093/femsec/fiy076

M3 - Article

C2 - 29718229

AN - SCOPUS:85050610447

VL - 94

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 7

M1 - fiy076

ER -

ID: 25358111