Syntrophobacter fumaroxidans strain MPOB^T is the best-studied species of the genus Syntrophobacter. The species is of interest because of its anaerobic syntrophic lifestyle, its in-volvement in the conversion of propionate to acetate, H₂ and CO₂ during the overall degra-dation of organic matter, and its release of products that serve as substrates for other microor-ganisms. The strain is able to ferment fumarate in pure culture to CO₂ and succinate, and is also able to grow as a sulfate reducer with propionate as an electron donor. This is the first complete genome sequence of a member of the genus Syntrophobacter and a member genus in the family Syntrophobacteraceae. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,990,251 bp long genome with its 4,098 protein-coding and 81 RNA genes is a part of the Microbial Genome Program (MGP) and the Genomes to Life (GTL) Program project.