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Comparison of different methods for generation of functional human cardiomyocytes. / Khudiakov, A. A.; Kurapeev, D. I.; Kostareva, A. A.; Malashicheva, A. B.

In: Cellular Transplantation and Tissue Engineering, Vol. 8, No. 2, 11.11.2013, p. 46-54.

Research output: Contribution to journal › Article › peer-review

Harvard

Khudiakov, AA, Kurapeev, DI, Kostareva, AA & Malashicheva, AB 2013, 'Comparison of different methods for generation of functional human cardiomyocytes', Cellular Transplantation and Tissue Engineering, vol. 8, no. 2, pp. 46-54.

APA

Khudiakov, A. A., Kurapeev, D. I., Kostareva, A. A., & Malashicheva, A. B. (2013). Comparison of different methods for generation of functional human cardiomyocytes. Cellular Transplantation and Tissue Engineering, 8(2), 46-54.

Vancouver

Khudiakov AA, Kurapeev DI, Kostareva AA, Malashicheva AB. Comparison of different methods for generation of functional human cardiomyocytes. Cellular Transplantation and Tissue Engineering. 2013 Nov 11;8(2):46-54.

Author

Khudiakov, A. A. ; Kurapeev, D. I. ; Kostareva, A. A. ; Malashicheva, A. B. / Comparison of different methods for generation of functional human cardiomyocytes. In: Cellular Transplantation and Tissue Engineering. 2013 ; Vol. 8, No. 2. pp. 46-54.

BibTeX

@article{8baf0508ad0243329f6b72630ef1d537,
title = "Comparison of different methods for generation of functional human cardiomyocytes",
abstract = "Generation of human cardiomyocytes in vitro is important for basic science and for regenerative medicine. There are a lot of published protocols describing the cardiomyocytes obtaining with different efficacy. However in practice, a researcher is faced with the fact that published methods have to be adapted to a particular laboratory for specific tasks. The aim of this study was to compare three methods for generation of human cardiomyocytes in vitro: 1) from human adult heart sample by purifying myocardial progenitor cells and their subsequent differentiation into cardiomyocyte direction; 2) from human adipose tissue multipotent mesenchimal stromal cells (AT-MSC); 3) from human dermal fibroblasts using reprogramming technology and subsequent differentiation to cardiomyocytes. We have shown relatively low efficiency of cardiac progenitor cells and AT-MSC capacity to give rise to cardiomyocyte lineage. The effectiveness of cardiomyocytes differentiation from dermal fibroblasts by reprogramming technology was significantly higher. The advantages, disadvantages and perspectives of each method are discussed.",
keywords = "Cardiomyocytes, Differentiation, Reprogramming",
author = "Khudiakov, {A. A.} and Kurapeev, {D. I.} and Kostareva, {A. A.} and Malashicheva, {A. B.}",
year = "2013",
month = nov,
day = "11",
language = "English",
volume = "8",
pages = "46--54",
journal = "Гены и клетки",
issn = "2313-1829",
publisher = "Институт стволовых клеток человека ",
number = "2",

}

RIS

TY - JOUR

T1 - Comparison of different methods for generation of functional human cardiomyocytes

AU - Khudiakov, A. A.

AU - Kurapeev, D. I.

AU - Kostareva, A. A.

AU - Malashicheva, A. B.

PY - 2013/11/11

Y1 - 2013/11/11

N2 - Generation of human cardiomyocytes in vitro is important for basic science and for regenerative medicine. There are a lot of published protocols describing the cardiomyocytes obtaining with different efficacy. However in practice, a researcher is faced with the fact that published methods have to be adapted to a particular laboratory for specific tasks. The aim of this study was to compare three methods for generation of human cardiomyocytes in vitro: 1) from human adult heart sample by purifying myocardial progenitor cells and their subsequent differentiation into cardiomyocyte direction; 2) from human adipose tissue multipotent mesenchimal stromal cells (AT-MSC); 3) from human dermal fibroblasts using reprogramming technology and subsequent differentiation to cardiomyocytes. We have shown relatively low efficiency of cardiac progenitor cells and AT-MSC capacity to give rise to cardiomyocyte lineage. The effectiveness of cardiomyocytes differentiation from dermal fibroblasts by reprogramming technology was significantly higher. The advantages, disadvantages and perspectives of each method are discussed.

AB - Generation of human cardiomyocytes in vitro is important for basic science and for regenerative medicine. There are a lot of published protocols describing the cardiomyocytes obtaining with different efficacy. However in practice, a researcher is faced with the fact that published methods have to be adapted to a particular laboratory for specific tasks. The aim of this study was to compare three methods for generation of human cardiomyocytes in vitro: 1) from human adult heart sample by purifying myocardial progenitor cells and their subsequent differentiation into cardiomyocyte direction; 2) from human adipose tissue multipotent mesenchimal stromal cells (AT-MSC); 3) from human dermal fibroblasts using reprogramming technology and subsequent differentiation to cardiomyocytes. We have shown relatively low efficiency of cardiac progenitor cells and AT-MSC capacity to give rise to cardiomyocyte lineage. The effectiveness of cardiomyocytes differentiation from dermal fibroblasts by reprogramming technology was significantly higher. The advantages, disadvantages and perspectives of each method are discussed.

KW - Cardiomyocytes

KW - Differentiation

KW - Reprogramming

UR - http://www.scopus.com/inward/record.url?scp=84887064572&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:84887064572

VL - 8

SP - 46

EP - 54

JO - Гены и клетки

JF - Гены и клетки

SN - 2313-1829

IS - 2

ER -

ID: 36015449