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Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue. / Krylova, T.A.; Bystrova, O.A.; Khudyakov, A.A.; Malashicheva, A.B.; Moiseeva, O.M.; Zenin, V.V.; Martynova, M.G.

In: Цитология, No. 3, 2014, p. 212-217.

Research output: Contribution to journal › Article

Harvard

Krylova, TA, Bystrova, OA, Khudyakov, AA, Malashicheva, AB, Moiseeva, OM, Zenin, VV & Martynova, MG 2014, 'Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue', Цитология, no. 3, pp. 212-217. <http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84904681380&origin=inward>

APA

Krylova, T. A., Bystrova, O. A., Khudyakov, A. A., Malashicheva, A. B., Moiseeva, O. M., Zenin, V. V., & Martynova, M. G. (2014). Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue. Цитология, (3), 212-217. http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84904681380&origin=inward

Vancouver

Krylova TA, Bystrova OA, Khudyakov AA, Malashicheva AB, Moiseeva OM, Zenin VV et al. Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue. Цитология. 2014;(3):212-217.

Author

Krylova, T.A. ; Bystrova, O.A. ; Khudyakov, A.A. ; Malashicheva, A.B. ; Moiseeva, O.M. ; Zenin, V.V. ; Martynova, M.G. / Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue. In: Цитология. 2014 ; No. 3. pp. 212-217.

BibTeX

@article{313be259c3d74487868a8a9385f0c2d7,
title = "Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue",
abstract = "Background. Stem cells (SCs) considerably vary in morphological, immunophenotypic, proliferative, and differentiation characteristics depending on their tissue source. The comparative analysis of their biological properties is essential for the optimal choice of SCs for regenerative therapies. Methods. Using immunocytochemistry, flow cytometry, histochemistry and real-time RT-PCR, we have investigated SCs obtained from human subepicardial (SEC-AT) and subcutaneous (SC-AT) adipose tissue and cultured under similar culture conditions without any differentiation-promoting factors. Results. The cultures were similar in the high proportion of proliferating cell nuclear antigen (PCNA)-positive cells. In both cultures, immunophenotyping has revealed high expression of mesenchymal stem cell surface markers CD29, CD44, CD73, and CD105, low expression of CD31, CD34 and CD45, and wide variability in CD117, CD146 and CD309 expression. The only distinction in CD marker profile was significantly lower expression of CD90 in",
author = "T.A. Krylova and O.A. Bystrova and A.A. Khudyakov and A.B. Malashicheva and O.M. Moiseeva and V.V. Zenin and M.G. Martynova",
year = "2014",
language = "русский",
pages = "212--217",
journal = "Цитология",
issn = "0041-3771",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "3",

}

RIS

TY - JOUR

T1 - Comparative characteristics of the stem cells isolated from subcutaneous and subepicardial adipose tissue

AU - Krylova, T.A.

AU - Bystrova, O.A.

AU - Khudyakov, A.A.

AU - Malashicheva, A.B.

AU - Moiseeva, O.M.

AU - Zenin, V.V.

AU - Martynova, M.G.

PY - 2014

Y1 - 2014

N2 - Background. Stem cells (SCs) considerably vary in morphological, immunophenotypic, proliferative, and differentiation characteristics depending on their tissue source. The comparative analysis of their biological properties is essential for the optimal choice of SCs for regenerative therapies. Methods. Using immunocytochemistry, flow cytometry, histochemistry and real-time RT-PCR, we have investigated SCs obtained from human subepicardial (SEC-AT) and subcutaneous (SC-AT) adipose tissue and cultured under similar culture conditions without any differentiation-promoting factors. Results. The cultures were similar in the high proportion of proliferating cell nuclear antigen (PCNA)-positive cells. In both cultures, immunophenotyping has revealed high expression of mesenchymal stem cell surface markers CD29, CD44, CD73, and CD105, low expression of CD31, CD34 and CD45, and wide variability in CD117, CD146 and CD309 expression. The only distinction in CD marker profile was significantly lower expression of CD90 in

AB - Background. Stem cells (SCs) considerably vary in morphological, immunophenotypic, proliferative, and differentiation characteristics depending on their tissue source. The comparative analysis of their biological properties is essential for the optimal choice of SCs for regenerative therapies. Methods. Using immunocytochemistry, flow cytometry, histochemistry and real-time RT-PCR, we have investigated SCs obtained from human subepicardial (SEC-AT) and subcutaneous (SC-AT) adipose tissue and cultured under similar culture conditions without any differentiation-promoting factors. Results. The cultures were similar in the high proportion of proliferating cell nuclear antigen (PCNA)-positive cells. In both cultures, immunophenotyping has revealed high expression of mesenchymal stem cell surface markers CD29, CD44, CD73, and CD105, low expression of CD31, CD34 and CD45, and wide variability in CD117, CD146 and CD309 expression. The only distinction in CD marker profile was significantly lower expression of CD90 in

M3 - статья

SP - 212

EP - 217

JO - Цитология

JF - Цитология

SN - 0041-3771

IS - 3

ER -

ID: 7064652