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Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins. / Markov, A.G.; Falchuk, E.L.; Kruglova, N.M.; Rybalchenko, O.V.; Fromm, M.; Amasheh, S.

In: Pflugers Archiv European Journal of Physiology, Vol. 466, No. 11, 2014, p. 2059-2065.

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Markov, A.G. ; Falchuk, E.L. ; Kruglova, N.M. ; Rybalchenko, O.V. ; Fromm, M. ; Amasheh, S. / Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins. In: Pflugers Archiv European Journal of Physiology. 2014 ; Vol. 466, No. 11. pp. 2059-2065.

BibTeX

@article{36ab9fdb9083415fac2f832b8d07b0eb,
title = "Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins",
abstract = "Claudin tight junction proteins have been identified to primarily determine intestinal epithelial barrier properties. Whereas functional contribution of single claudins have been characterized in detail, information on the interplay with secretory mechanisms in native intestinal epithelium is scarce. Therefore, effects of cholera toxin and theophylline on rat colon were analyzed, including detection of sealing claudins. Tissue specimens were stripped off submucosal tissue layers and mounted in Ussing chambers. Short circuit current (ISC) and transepithelial resistance (TER) were reported, and expression and localization of claudins was analyzed. Moreover, histological analyses were performed, employing hematoxylin-eosin staining and light and electron microscopy. Theophylline induced a strong increase of ISC in colon tissue specimens. In parallel, a decrease of TER was observed. In contrast, cholera toxin did not induce a significant increase of ISC, whereas an increase of TER was detected after 120 min. West",
keywords = "эпителий, тканевые барьеры, межклеточный транспорт, холерный токсин, теофиллин, плотные контакты, клаудины, кишка, крыса",
author = "A.G. Markov and E.L. Falchuk and N.M. Kruglova and O.V. Rybalchenko and M. Fromm and S. Amasheh",
year = "2014",
doi = "10.1007/s00424-014-1460-z",
language = "English",
volume = "466",
pages = "2059--2065",
journal = "Pflugers Archiv European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Nature",
number = "11",

}

RIS

TY - JOUR

T1 - Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins

AU - Markov, A.G.

AU - Falchuk, E.L.

AU - Kruglova, N.M.

AU - Rybalchenko, O.V.

AU - Fromm, M.

AU - Amasheh, S.

PY - 2014

Y1 - 2014

N2 - Claudin tight junction proteins have been identified to primarily determine intestinal epithelial barrier properties. Whereas functional contribution of single claudins have been characterized in detail, information on the interplay with secretory mechanisms in native intestinal epithelium is scarce. Therefore, effects of cholera toxin and theophylline on rat colon were analyzed, including detection of sealing claudins. Tissue specimens were stripped off submucosal tissue layers and mounted in Ussing chambers. Short circuit current (ISC) and transepithelial resistance (TER) were reported, and expression and localization of claudins was analyzed. Moreover, histological analyses were performed, employing hematoxylin-eosin staining and light and electron microscopy. Theophylline induced a strong increase of ISC in colon tissue specimens. In parallel, a decrease of TER was observed. In contrast, cholera toxin did not induce a significant increase of ISC, whereas an increase of TER was detected after 120 min. West

AB - Claudin tight junction proteins have been identified to primarily determine intestinal epithelial barrier properties. Whereas functional contribution of single claudins have been characterized in detail, information on the interplay with secretory mechanisms in native intestinal epithelium is scarce. Therefore, effects of cholera toxin and theophylline on rat colon were analyzed, including detection of sealing claudins. Tissue specimens were stripped off submucosal tissue layers and mounted in Ussing chambers. Short circuit current (ISC) and transepithelial resistance (TER) were reported, and expression and localization of claudins was analyzed. Moreover, histological analyses were performed, employing hematoxylin-eosin staining and light and electron microscopy. Theophylline induced a strong increase of ISC in colon tissue specimens. In parallel, a decrease of TER was observed. In contrast, cholera toxin did not induce a significant increase of ISC, whereas an increase of TER was detected after 120 min. West

KW - эпителий

KW - тканевые барьеры

KW - межклеточный транспорт

KW - холерный токсин

KW - теофиллин

KW - плотные контакты

KW - клаудины

KW - кишка

KW - крыса

U2 - 10.1007/s00424-014-1460-z

DO - 10.1007/s00424-014-1460-z

M3 - Article

VL - 466

SP - 2059

EP - 2065

JO - Pflugers Archiv European Journal of Physiology

JF - Pflugers Archiv European Journal of Physiology

SN - 0031-6768

IS - 11

ER -

ID: 6993883