Research output: Contribution to journal › Article
Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins. / Markov, A.G.; Falchuk, E.L.; Kruglova, N.M.; Rybalchenko, O.V.; Fromm, M.; Amasheh, S.
In: Pflugers Archiv European Journal of Physiology, Vol. 466, No. 11, 2014, p. 2059-2065.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Comparative analysis of theophylline and cholera toxin in rat colon reveals an induction of sealing tight junction proteins
AU - Markov, A.G.
AU - Falchuk, E.L.
AU - Kruglova, N.M.
AU - Rybalchenko, O.V.
AU - Fromm, M.
AU - Amasheh, S.
PY - 2014
Y1 - 2014
N2 - Claudin tight junction proteins have been identified to primarily determine intestinal epithelial barrier properties. Whereas functional contribution of single claudins have been characterized in detail, information on the interplay with secretory mechanisms in native intestinal epithelium is scarce. Therefore, effects of cholera toxin and theophylline on rat colon were analyzed, including detection of sealing claudins. Tissue specimens were stripped off submucosal tissue layers and mounted in Ussing chambers. Short circuit current (ISC) and transepithelial resistance (TER) were reported, and expression and localization of claudins was analyzed. Moreover, histological analyses were performed, employing hematoxylin-eosin staining and light and electron microscopy. Theophylline induced a strong increase of ISC in colon tissue specimens. In parallel, a decrease of TER was observed. In contrast, cholera toxin did not induce a significant increase of ISC, whereas an increase of TER was detected after 120 min. West
AB - Claudin tight junction proteins have been identified to primarily determine intestinal epithelial barrier properties. Whereas functional contribution of single claudins have been characterized in detail, information on the interplay with secretory mechanisms in native intestinal epithelium is scarce. Therefore, effects of cholera toxin and theophylline on rat colon were analyzed, including detection of sealing claudins. Tissue specimens were stripped off submucosal tissue layers and mounted in Ussing chambers. Short circuit current (ISC) and transepithelial resistance (TER) were reported, and expression and localization of claudins was analyzed. Moreover, histological analyses were performed, employing hematoxylin-eosin staining and light and electron microscopy. Theophylline induced a strong increase of ISC in colon tissue specimens. In parallel, a decrease of TER was observed. In contrast, cholera toxin did not induce a significant increase of ISC, whereas an increase of TER was detected after 120 min. West
KW - эпителий
KW - тканевые барьеры
KW - межклеточный транспорт
KW - холерный токсин
KW - теофиллин
KW - плотные контакты
KW - клаудины
KW - кишка
KW - крыса
U2 - 10.1007/s00424-014-1460-z
DO - 10.1007/s00424-014-1460-z
M3 - Article
VL - 466
SP - 2059
EP - 2065
JO - Pflugers Archiv European Journal of Physiology
JF - Pflugers Archiv European Journal of Physiology
SN - 0031-6768
IS - 11
ER -
ID: 6993883